Reviewed, UniProtKB/Swiss-Prot Q49901 (SYW_MYCLE)

Names and origin

Protein names

Recommended name:
    Tryptophanyl-tRNA synthetase
    EC=6.1.1.2
Alternative name(s):
    Tryptophan--tRNA ligase
      Short name=TrpRS

Gene names

Name:	trpS
Ordered Locus Names:	ML0686
ORF Names:	L308_C1_147

Organism

Mycobacterium leprae [Complete proteome] [HAMAP]

Taxonomic identifier

1769 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp). HAMAP MF_00140
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00140
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tryptophanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP tryptophan-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 343	343	Tryptophanyl-tRNA synthetase HAMAP MF_00140		PRO_0000136650
Regions
	Motif	16 – 25	10	"HIGH" region HAMAP MF_00140
	Motif	205 – 209	5	"KMSKS" region HAMAP MF_00140
Sites
	Binding site	208	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q49901-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: C0CD552AA6B02D88	FASTA	343	37,608
	10 20 30 40 50 60 MSTATGFSRI FSGVQPTSDS LHLGNALGAI TQWVALQYDH PDEYEAFFCV VDLHAITIAQ 70 80 90 100 110 120 DPETLWRRTL VTAAQYLALG IDPGRAVVFV QSHVPAHTQL AWVLGCFTGF GQASRMTQFK 130 140 150 160 170 180 DKALRQGADS TTVGLFTYPV LQAADVLAYD TDLVPVGEDQ RQHLELARDL AQRFNSRFPD 190 200 210 220 230 240 TFVVPDMFIP KMAAKIYDLA DPTSKMSKSA SSDAGLINLL DDPALSVKKI RAAVTDSERE 250 260 270 280 290 300 IRYDPEVKPG VSNLLNIQSA VTGVDVDTLV QRYVGHGYGD LKKDTAEAVV EFVSPIKDRV 310 320 330 340 DELMADLTEL EVVLAVGAQR AQDVAGKTMQ RVYDRLGFLP QRG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Smith D.R., Robison K. Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Massive gene decay in the leprosy bacillus." Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S. , Feltwell T., Fraser A., Hamlin N., Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S., Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R., Barrell B.G. Nature 409:1007-1011(2001) [PubMed: 11234002] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TN.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U00022 Genomic DNA. Translation: AAA17323.1. AL583919 Genomic DNA. Translation: CAC30195.1.
PIR	S73024.
RefSeq	NP_301552.1.
3D structure databases
HSSP	HSSP built from PDB template 1MAU based on UniProtKB P00953.
ModBase	Search...
Genome annotation databases
GeneID	909578.
GenomeReviews	Gene locus ML0686 in contig AL450380_GR.
KEGG	mle:ML0686.
NMPDR	fig|272631.1.peg.424.
Organism-specific databases
Leproma	ML0686.
CMR	Search...
Phylogenomic databases
HOGENOM	Q49901.
OMA	Q49901. NKPGVSN.
Enzyme and pathway databases
BioCyc	MLEP272631:ML0686-MON.
BRENDA	6.1.1.2. 808.
Family and domain databases
HAMAP	MF_00140. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ib. IPR014729. Rossmann-like_a/b/a_fold. IPR002306. Trp-tRNA-synth_Ib. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR10055. Trp_tRNA-synt_1b. 1 hit.
Pfam	PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01039. TRNASYNTHTRP.
TIGRFAMs	TIGR00233. trpS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYW_MYCLE

Accession

Primary (citable) accession number: Q49901

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)