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Protein

Tryptophan--tRNA ligase

Gene

trpS

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of tryptophan to tRNA(Trp).UniRule annotation

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei145L-tryptophanUniRule annotation1
Binding sitei196ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi15 – 17ATPUniRule annotation3
Nucleotide bindingi24 – 25ATPUniRule annotation2
Nucleotide bindingi157 – 159ATPUniRule annotation3
Nucleotide bindingi205 – 209ATPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMLEP272631:G1GT5-750-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligaseUniRule annotation (EC:6.1.1.2UniRule annotation)
Alternative name(s):
Tryptophanyl-tRNA synthetaseUniRule annotation
Short name:
TrpRSUniRule annotation
Gene namesi
Name:trpSUniRule annotation
Ordered Locus Names:ML0686
ORF Names:L308_C1_147
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000806 Componenti: Chromosome

Organism-specific databases

LepromaiML0686

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001366501 – 343Tryptophan--tRNA ligaseAdd BLAST343

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi272631.ML0686

Structurei

3D structure databases

ProteinModelPortaliQ49901
SMRiQ49901
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi16 – 25"HIGH" regionUniRule annotation10
Motifi205 – 209"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C31 Bacteria
COG0180 LUCA
HOGENOMiHOG000059940
KOiK01867
OMAiQRMTQFK
OrthoDBiPOG091H00I9

Family and domain databases

CDDicd00806 TrpRS_core, 1 hit
Gene3Di3.40.50.620, 2 hits
HAMAPiMF_00140_B Trp_tRNA_synth_B, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002305 aa-tRNA-synth_Ic
IPR014729 Rossmann-like_a/b/a_fold
IPR002306 Trp-tRNA-ligase
IPR024109 Trp-tRNA-ligase_bac-type
PfamiView protein in Pfam
PF00579 tRNA-synt_1b, 1 hit
PRINTSiPR01039 TRNASYNTHTRP
TIGRFAMsiTIGR00233 trpS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q49901-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTATGFSRI FSGVQPTSDS LHLGNALGAI TQWVALQYDH PDEYEAFFCV
60 70 80 90 100
VDLHAITIAQ DPETLWRRTL VTAAQYLALG IDPGRAVVFV QSHVPAHTQL
110 120 130 140 150
AWVLGCFTGF GQASRMTQFK DKALRQGADS TTVGLFTYPV LQAADVLAYD
160 170 180 190 200
TDLVPVGEDQ RQHLELARDL AQRFNSRFPD TFVVPDMFIP KMAAKIYDLA
210 220 230 240 250
DPTSKMSKSA SSDAGLINLL DDPALSVKKI RAAVTDSERE IRYDPEVKPG
260 270 280 290 300
VSNLLNIQSA VTGVDVDTLV QRYVGHGYGD LKKDTAEAVV EFVSPIKDRV
310 320 330 340
DELMADLTEL EVVLAVGAQR AQDVAGKTMQ RVYDRLGFLP QRG
Length:343
Mass (Da):37,608
Last modified:November 1, 1996 - v1
Checksum:iC0CD552AA6B02D88
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00022 Genomic DNA Translation: AAA17323.1
AL583919 Genomic DNA Translation: CAC30195.1
PIRiS73024
RefSeqiNP_301552.1, NC_002677.1
WP_010907876.1, NC_002677.1

Genome annotation databases

EnsemblBacteriaiCAC30195; CAC30195; CAC30195
GeneIDi909578
KEGGimle:ML0686
PATRICifig|272631.5.peg.1226

Similar proteinsi

Entry informationi

Entry nameiSYW_MYCLE
AccessioniPrimary (citable) accession number: Q49901
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: March 28, 2018
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health