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Protein

Tyrosine--tRNA ligase

Gene

tyrS

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).UniRule annotation

Catalytic activityi

ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei36TyrosineUniRule annotation1
Binding sitei174TyrosineUniRule annotation1
Binding sitei178TyrosineUniRule annotation1
Binding sitei237ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine--tRNA ligaseUniRule annotation (EC:6.1.1.1UniRule annotation)
Alternative name(s):
Tyrosyl-tRNA synthetaseUniRule annotation
Short name:
TyrRSUniRule annotation
Gene namesi
Name:tyrSUniRule annotation
Ordered Locus Names:ML1352
ORF Names:MLC1351.16c
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000806 Componenti: Chromosome

Organism-specific databases

LepromaiML1352.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000556591 – 426Tyrosine--tRNA ligaseAdd BLAST426

Proteomic databases

PRIDEiQ49900.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi272631.ML1352.

Structurei

3D structure databases

ProteinModelPortaliQ49900.
SMRiQ49900.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini359 – 416S4 RNA-bindingUniRule annotationAdd BLAST58

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi41 – 50"HIGH" region10
Motifi234 – 238"KMSKS" region5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 1 subfamily.UniRule annotation
Contains 1 S4 RNA-binding domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DA0. Bacteria.
COG0162. LUCA.
HOGENOMiHOG000242790.
KOiK01866.
OMAiGKHFPVN.
OrthoDBiPOG091H00WT.

Family and domain databases

CDDicd00805. TyrRS_core. 1 hit.
Gene3Di3.10.290.10. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_02006. Tyr_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002942. S4_RNA-bd.
IPR002307. Tyr-tRNA-ligase.
IPR024088. Tyr-tRNA-ligase_bac-type.
IPR024107. Tyr-tRNA-ligase_bac_1.
[Graphical view]
PANTHERiPTHR11766. PTHR11766. 1 hit.
PfamiPF01479. S4. 1 hit.
PF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01040. TRNASYNTHTYR.
SMARTiSM00363. S4. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00234. tyrS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50889. S4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q49900-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSILNELG WRGLVAQSTD LDALADELRR GPMTVYAGFD PTAPSLHVGH
60 70 80 90 100
LVPLLVLRRF QRAGHRPIVL AGGATGMIGD PRDIGERTLN EAYTVAEWAE
110 120 130 140 150
RIRGQLERFV EFDDVAKSRN GAIVVNNLEW TSPMSAIEFL RDVGKHFSIN
160 170 180 190 200
LMLDRDTIRR RLNGQGISYT EFSYMLLQAN DYAELHQRHG CALQIGGSDQ
210 220 230 240 250
WGNIIAGVRL VRQKLGATVH ALTVPLVTAA DGAKLGKSTG GGSLWLDPEM
260 270 280 290 300
TSPYAWYQYF INTSDADVIR YLRWFTFLLP EELVELEQTT VSRPQERAAQ
310 320 330 340 350
RRLATELTVL VHGAAATQAV EHASRALFGQ GELARLDEAT LATALRETVV
360 370 380 390 400
AELKPGNPDG IVDLLVASGL SPSRGAARRT IDEGGVLVNN IRIQSEEWTP
410 420
RTSDFLHGRW LVLRRGKRNI AGIERV
Length:426
Mass (Da):47,049
Last modified:April 27, 2001 - v3
Checksum:i0BAB35229E6277EE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z95117 Genomic DNA. Translation: CAB08289.1.
AL583921 Genomic DNA. Translation: CAC31733.1.
U00021 Genomic DNA. Translation: AAA50922.1.
PIRiB87078.
S72970.
RefSeqiNP_301966.1. NC_002677.1.
WP_010908287.1. NC_002677.1.

Genome annotation databases

EnsemblBacteriaiCAC31733; CAC31733; CAC31733.
GeneIDi910480.
KEGGimle:ML1352.
PATRICi18055136. VBIMycLep78757_2502.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z95117 Genomic DNA. Translation: CAB08289.1.
AL583921 Genomic DNA. Translation: CAC31733.1.
U00021 Genomic DNA. Translation: AAA50922.1.
PIRiB87078.
S72970.
RefSeqiNP_301966.1. NC_002677.1.
WP_010908287.1. NC_002677.1.

3D structure databases

ProteinModelPortaliQ49900.
SMRiQ49900.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272631.ML1352.

Proteomic databases

PRIDEiQ49900.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC31733; CAC31733; CAC31733.
GeneIDi910480.
KEGGimle:ML1352.
PATRICi18055136. VBIMycLep78757_2502.

Organism-specific databases

LepromaiML1352.

Phylogenomic databases

eggNOGiENOG4105DA0. Bacteria.
COG0162. LUCA.
HOGENOMiHOG000242790.
KOiK01866.
OMAiGKHFPVN.
OrthoDBiPOG091H00WT.

Family and domain databases

CDDicd00805. TyrRS_core. 1 hit.
Gene3Di3.10.290.10. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_02006. Tyr_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002942. S4_RNA-bd.
IPR002307. Tyr-tRNA-ligase.
IPR024088. Tyr-tRNA-ligase_bac-type.
IPR024107. Tyr-tRNA-ligase_bac_1.
[Graphical view]
PANTHERiPTHR11766. PTHR11766. 1 hit.
PfamiPF01479. S4. 1 hit.
PF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01040. TRNASYNTHTYR.
SMARTiSM00363. S4. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00234. tyrS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50889. S4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYY_MYCLE
AccessioniPrimary (citable) accession number: Q49900
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: April 27, 2001
Last modified: November 30, 2016
This is version 107 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.