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Q498S8

- FZD8_RAT

UniProt

Q498S8 - FZD8_RAT

Protein

Frizzled-8

Gene

Fzd8

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (13 Sep 2005)
      Previous versions | rss
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    Functioni

    Receptor for Wnt proteins. Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalsomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Coreceptor along with RYK of Wnt proteins, such as WNT1 By similarity.By similarity

    GO - Molecular functioni

    1. G-protein coupled receptor activity Source: UniProtKB-KW
    2. PDZ domain binding Source: RefGenome
    3. Wnt-activated receptor activity Source: RefGenome
    4. Wnt-protein binding Source: RefGenome

    GO - Biological processi

    1. axonogenesis Source: RefGenome
    2. brain development Source: RefGenome
    3. canonical Wnt signaling pathway Source: RefGenome
    4. gonad development Source: RefGenome
    5. T cell differentiation in thymus Source: RefGenome
    6. vasculature development Source: RefGenome

    Keywords - Molecular functioni

    Developmental protein, G-protein coupled receptor, Receptor, Transducer

    Keywords - Biological processi

    Wnt signaling pathway

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Frizzled-8
    Short name:
    Fz-8
    Gene namesi
    Name:Fzd8
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 17

    Organism-specific databases

    RGDi1560525. Fzd8.

    Subcellular locationi

    Membrane; Multi-pass membrane protein. Golgi apparatus By similarity. Cell membrane By similarity; Multi-pass membrane protein By similarity
    Note: Colocalizes with GOPC at the Golgi apparatus.By similarity

    GO - Cellular componenti

    1. cell projection Source: RefGenome
    2. cytoplasm Source: RefGenome
    3. Golgi apparatus Source: UniProtKB-SubCell
    4. integral component of membrane Source: UniProtKB-KW
    5. plasma membrane Source: RefGenome

    Keywords - Cellular componenti

    Cell membrane, Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2727Sequence AnalysisAdd
    BLAST
    Chaini28 – 684657Frizzled-8PRO_0000278650Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi35 ↔ 96PROSITE-ProRule annotation
    Disulfide bondi43 ↔ 89PROSITE-ProRule annotation
    Glycosylationi49 – 491N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi80 ↔ 118PROSITE-ProRule annotation
    Disulfide bondi107 ↔ 148PROSITE-ProRule annotation
    Disulfide bondi111 ↔ 135PROSITE-ProRule annotation
    Glycosylationi152 – 1521N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi472 – 4721N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Ubl conjugation

    Proteomic databases

    PaxDbiQ498S8.
    PRIDEiQ498S8.

    PTM databases

    PhosphoSiteiQ498S8.

    Expressioni

    Gene expression databases

    GenevestigatoriQ498S8.

    Interactioni

    Subunit structurei

    Component of a Wnt-signaling complex that contains a WNT protein, a FZD protein and LRP5 or LRP6. Interacts directly with LRP5 or LRP6; the interaction is promoted by Wnt-binding and signaling and inhibited by DKK1. Interacts (via the PDZ-binding motif) with GPOC (via its PDZ domain). Interacts with RSPO1 and RSPO3 By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ498S8.
    SMRiQ498S8. Positions 32-153.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini28 – 271244ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini293 – 30816CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini330 – 39364ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini415 – 43622CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini458 – 48023ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini502 – 52928CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini551 – 58131ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini603 – 68482CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei272 – 29221Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei309 – 32921Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei394 – 41421Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei437 – 45721Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei481 – 50121Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei530 – 55021Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei582 – 60221Helical; Name=7Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini30 – 151122FZPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni71 – 788Palmitate-binding grooveBy similarity
    Regioni95 – 1006Wnt-bindingBy similarity
    Regioni147 – 1526Wnt-bindingBy similarity

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi605 – 6106Lys-Thr-X-X-X-Trp motif, mediates interaction with the PDZ domain of Dvl family membersBy similarity
    Motifi682 – 6843PDZ-bindingBy similarity

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi161 – 22666Pro-richAdd
    BLAST
    Compositional biasi168 – 1736Poly-Pro
    Compositional biasi639 – 65315Poly-GlyAdd
    BLAST

    Domaini

    Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.By similarity
    The FZ domain is involved in binding with Wnt ligands.By similarity

    Sequence similaritiesi

    Contains 1 FZ (frizzled) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG257258.
    GeneTreeiENSGT00750000117488.
    HOGENOMiHOG000233236.
    HOVERGENiHBG006977.
    InParanoidiQ498S8.
    KOiK02375.
    OMAiHEKVACN.
    OrthoDBiEOG7M3J01.
    PhylomeDBiQ498S8.
    TreeFamiTF317907.

    Family and domain databases

    Gene3Di1.10.2000.10. 1 hit.
    InterProiIPR000539. Frizzled.
    IPR015526. Frizzled/SFRP.
    IPR020067. Frizzled_dom.
    IPR017981. GPCR_2-like.
    [Graphical view]
    PANTHERiPTHR11309. PTHR11309. 1 hit.
    PfamiPF01534. Frizzled. 1 hit.
    PF01392. Fz. 1 hit.
    [Graphical view]
    PRINTSiPR00489. FRIZZLED.
    SMARTiSM00063. FRI. 1 hit.
    [Graphical view]
    SUPFAMiSSF63501. SSF63501. 1 hit.
    PROSITEiPS50038. FZ. 1 hit.
    PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q498S8-1 [UniParc]FASTAAdd to Basket

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    MEWGYLLEVT SLLAALAVLQ RSSGAAAASA KELACQEITV PLCKGIGYNY    50
    TYMPNQFNHD TQDEAGLEVH QFWPLVEIQC SPDLKFFLCS MYTPICLEDY 100
    KKPLPPCRSV CERAKAGCAP LMRQYGFAWP DRMRCDRLPE QGNPDTLCMD 150
    YNRTDLTTAA PSPPRRLPPP QPGEQPPSGS GHSRPPGARP PHRGGSSRGS 200
    GDTAAAPPSR GGKARPPGGG AAPCEPGCQC RAPMVSVSSE RHPLYNRVKT 250
    GQIANCALPC HNPFFSQDER AFTVFWIGLW SVLCFVSTFA TVSTFLIDME 300
    RFKYPERPII FLSACYLFVS VGYLVRLVAG HEKVACSGGA PGAGGAGGAG 350
    GAATAGAGAA GAGASSPGAR GEYEELGAVE QHVRYETTGP ALCTVVFLLV 400
    YFFGMASSIW WVILSLTWFL AAGMKWGNEA IAGYSQYFHL AAWLVPSVKS 450
    IAVLALSSVD GDPVAGICYV GNQSLDNLRG FVLAPLVIYL FIGTMFLLAG 500
    FVSLFRIRSV IKQQGGPTKT HKLEKLMIRL GLFTVLYTVP AAVVVACLFY 550
    EQHNRPRWEA THNCPCLRDL QPDQARRPDY AVFMLKYFMC LVVGITSGVW 600
    VWSGKTLESW RALCTRCCWA SKGAAVGAGA GGSGPGGGGP GPGGGGGHGG 650
    GGGSLYSDVS TGLTWRSGTA SSVSYPKQMP LSQV 684
    Length:684
    Mass (Da):73,009
    Last modified:September 13, 2005 - v1
    Checksum:iEEBA871326749B69
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC100088 mRNA. Translation: AAI00089.1.
    RefSeqiNP_001037716.1. NM_001044251.2.
    XP_003751769.1. XM_003751721.2.
    XP_006254204.1. XM_006254142.1.
    UniGeneiRn.234505.

    Genome annotation databases

    EnsembliENSRNOT00000058837; ENSRNOP00000055627; ENSRNOG00000038571.
    ENSRNOT00000073575; ENSRNOP00000067651; ENSRNOG00000048236.
    GeneIDi100909849.
    364754.
    KEGGirno:100909849.
    rno:364754.
    UCSCiRGD:1560525. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC100088 mRNA. Translation: AAI00089.1 .
    RefSeqi NP_001037716.1. NM_001044251.2.
    XP_003751769.1. XM_003751721.2.
    XP_006254204.1. XM_006254142.1.
    UniGenei Rn.234505.

    3D structure databases

    ProteinModelPortali Q498S8.
    SMRi Q498S8. Positions 32-153.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei Q498S8.

    Proteomic databases

    PaxDbi Q498S8.
    PRIDEi Q498S8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000058837 ; ENSRNOP00000055627 ; ENSRNOG00000038571 .
    ENSRNOT00000073575 ; ENSRNOP00000067651 ; ENSRNOG00000048236 .
    GeneIDi 100909849.
    364754.
    KEGGi rno:100909849.
    rno:364754.
    UCSCi RGD:1560525. rat.

    Organism-specific databases

    CTDi 8325.
    RGDi 1560525. Fzd8.

    Phylogenomic databases

    eggNOGi NOG257258.
    GeneTreei ENSGT00750000117488.
    HOGENOMi HOG000233236.
    HOVERGENi HBG006977.
    InParanoidi Q498S8.
    KOi K02375.
    OMAi HEKVACN.
    OrthoDBi EOG7M3J01.
    PhylomeDBi Q498S8.
    TreeFami TF317907.

    Miscellaneous databases

    NextBioi 685973.
    PROi Q498S8.

    Gene expression databases

    Genevestigatori Q498S8.

    Family and domain databases

    Gene3Di 1.10.2000.10. 1 hit.
    InterProi IPR000539. Frizzled.
    IPR015526. Frizzled/SFRP.
    IPR020067. Frizzled_dom.
    IPR017981. GPCR_2-like.
    [Graphical view ]
    PANTHERi PTHR11309. PTHR11309. 1 hit.
    Pfami PF01534. Frizzled. 1 hit.
    PF01392. Fz. 1 hit.
    [Graphical view ]
    PRINTSi PR00489. FRIZZLED.
    SMARTi SM00063. FRI. 1 hit.
    [Graphical view ]
    SUPFAMi SSF63501. SSF63501. 1 hit.
    PROSITEi PS50038. FZ. 1 hit.
    PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Liver.

    Entry informationi

    Entry nameiFZD8_RAT
    AccessioniPrimary (citable) accession number: Q498S8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 20, 2007
    Last sequence update: September 13, 2005
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3