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Q498S8

- FZD8_RAT

UniProt

Q498S8 - FZD8_RAT

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Protein

Frizzled-8

Gene
Fzd8
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Receptor for Wnt proteins. Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalsomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Coreceptor along with RYK of Wnt proteins, such as WNT1 By similarity.

GO - Molecular functioni

  1. G-protein coupled receptor activity Source: UniProtKB-KW
  2. PDZ domain binding Source: RefGenome
  3. Wnt-activated receptor activity Source: RefGenome
  4. Wnt-protein binding Source: RefGenome

GO - Biological processi

  1. axonogenesis Source: RefGenome
  2. brain development Source: RefGenome
  3. canonical Wnt signaling pathway Source: RefGenome
  4. gonad development Source: RefGenome
  5. T cell differentiation in thymus Source: RefGenome
  6. vasculature development Source: RefGenome
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Wnt signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Frizzled-8
Short name:
Fz-8
Gene namesi
Name:Fzd8
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 17

Organism-specific databases

RGDi1560525. Fzd8.

Subcellular locationi

Membrane; Multi-pass membrane protein. Golgi apparatus By similarity. Cell membrane; Multi-pass membrane protein By similarity
Note: Colocalizes with GOPC at the Golgi apparatus By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini28 – 271244Extracellular Reviewed predictionAdd
BLAST
Transmembranei272 – 29221Helical; Name=1; Reviewed predictionAdd
BLAST
Topological domaini293 – 30816Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei309 – 32921Helical; Name=2; Reviewed predictionAdd
BLAST
Topological domaini330 – 39364Extracellular Reviewed predictionAdd
BLAST
Transmembranei394 – 41421Helical; Name=3; Reviewed predictionAdd
BLAST
Topological domaini415 – 43622Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei437 – 45721Helical; Name=4; Reviewed predictionAdd
BLAST
Topological domaini458 – 48023Extracellular Reviewed predictionAdd
BLAST
Transmembranei481 – 50121Helical; Name=5; Reviewed predictionAdd
BLAST
Topological domaini502 – 52928Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei530 – 55021Helical; Name=6; Reviewed predictionAdd
BLAST
Topological domaini551 – 58131Extracellular Reviewed predictionAdd
BLAST
Transmembranei582 – 60221Helical; Name=7; Reviewed predictionAdd
BLAST
Topological domaini603 – 68482Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. cell projection Source: RefGenome
  2. cytoplasm Source: RefGenome
  3. Golgi apparatus Source: UniProtKB-SubCell
  4. integral component of membrane Source: UniProtKB-KW
  5. plasma membrane Source: RefGenome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727 Reviewed predictionAdd
BLAST
Chaini28 – 684657Frizzled-8PRO_0000278650Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi35 ↔ 96 By similarity
Disulfide bondi43 ↔ 89 By similarity
Glycosylationi49 – 491N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi80 ↔ 118 By similarity
Disulfide bondi107 ↔ 148 By similarity
Disulfide bondi111 ↔ 135 By similarity
Glycosylationi152 – 1521N-linked (GlcNAc...) Reviewed prediction
Glycosylationi472 – 4721N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

Ubiquitinated by ZNRF3, leading to its degradation by the proteasome By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ498S8.
PRIDEiQ498S8.

PTM databases

PhosphoSiteiQ498S8.

Expressioni

Gene expression databases

GenevestigatoriQ498S8.

Interactioni

Subunit structurei

Component of a Wnt-signaling complex that contains a WNT protein, a FZD protein and LRP5 or LRP6. Interacts directly with LRP5 or LRP6; the interaction is promoted by Wnt-binding and signaling and inhibited by DKK1. Interacts (via the PDZ-binding motif) with GPOC (via its PDZ domain). Interacts with RSPO1 and RSPO3 By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ498S8.
SMRiQ498S8. Positions 32-153.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini30 – 151122FZAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni71 – 788Palmitate-binding groove By similarity
Regioni95 – 1006Wnt-binding By similarity
Regioni147 – 1526Wnt-binding By similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi605 – 6106Lys-Thr-X-X-X-Trp motif, mediates interaction with the PDZ domain of Dvl family members By similarity
Motifi682 – 6843PDZ-binding By similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi161 – 22666Pro-richAdd
BLAST
Compositional biasi168 – 1736Poly-Pro
Compositional biasi639 – 65315Poly-GlyAdd
BLAST

Domaini

Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway By similarity.
The FZ domain is involved in binding with Wnt ligands By similarity.

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG257258.
GeneTreeiENSGT00750000117488.
HOGENOMiHOG000233236.
HOVERGENiHBG006977.
InParanoidiQ498S8.
KOiK02375.
OMAiHEKVACN.
OrthoDBiEOG7M3J01.
PhylomeDBiQ498S8.
TreeFamiTF317907.

Family and domain databases

Gene3Di1.10.2000.10. 1 hit.
InterProiIPR000539. Frizzled.
IPR015526. Frizzled/SFRP.
IPR020067. Frizzled_dom.
IPR017981. GPCR_2-like.
[Graphical view]
PANTHERiPTHR11309. PTHR11309. 1 hit.
PfamiPF01534. Frizzled. 1 hit.
PF01392. Fz. 1 hit.
[Graphical view]
PRINTSiPR00489. FRIZZLED.
SMARTiSM00063. FRI. 1 hit.
[Graphical view]
SUPFAMiSSF63501. SSF63501. 1 hit.
PROSITEiPS50038. FZ. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q498S8-1 [UniParc]FASTAAdd to Basket

« Hide

MEWGYLLEVT SLLAALAVLQ RSSGAAAASA KELACQEITV PLCKGIGYNY    50
TYMPNQFNHD TQDEAGLEVH QFWPLVEIQC SPDLKFFLCS MYTPICLEDY 100
KKPLPPCRSV CERAKAGCAP LMRQYGFAWP DRMRCDRLPE QGNPDTLCMD 150
YNRTDLTTAA PSPPRRLPPP QPGEQPPSGS GHSRPPGARP PHRGGSSRGS 200
GDTAAAPPSR GGKARPPGGG AAPCEPGCQC RAPMVSVSSE RHPLYNRVKT 250
GQIANCALPC HNPFFSQDER AFTVFWIGLW SVLCFVSTFA TVSTFLIDME 300
RFKYPERPII FLSACYLFVS VGYLVRLVAG HEKVACSGGA PGAGGAGGAG 350
GAATAGAGAA GAGASSPGAR GEYEELGAVE QHVRYETTGP ALCTVVFLLV 400
YFFGMASSIW WVILSLTWFL AAGMKWGNEA IAGYSQYFHL AAWLVPSVKS 450
IAVLALSSVD GDPVAGICYV GNQSLDNLRG FVLAPLVIYL FIGTMFLLAG 500
FVSLFRIRSV IKQQGGPTKT HKLEKLMIRL GLFTVLYTVP AAVVVACLFY 550
EQHNRPRWEA THNCPCLRDL QPDQARRPDY AVFMLKYFMC LVVGITSGVW 600
VWSGKTLESW RALCTRCCWA SKGAAVGAGA GGSGPGGGGP GPGGGGGHGG 650
GGGSLYSDVS TGLTWRSGTA SSVSYPKQMP LSQV 684
Length:684
Mass (Da):73,009
Last modified:September 13, 2005 - v1
Checksum:iEEBA871326749B69
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC100088 mRNA. Translation: AAI00089.1.
RefSeqiNP_001037716.1. NM_001044251.2.
XP_003751769.1. XM_003751721.2.
XP_006254204.1. XM_006254142.1.
UniGeneiRn.234505.

Genome annotation databases

EnsembliENSRNOT00000058837; ENSRNOP00000055627; ENSRNOG00000038571.
ENSRNOT00000073575; ENSRNOP00000067651; ENSRNOG00000048236.
GeneIDi100909849.
364754.
KEGGirno:100909849.
rno:364754.
UCSCiRGD:1560525. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC100088 mRNA. Translation: AAI00089.1 .
RefSeqi NP_001037716.1. NM_001044251.2.
XP_003751769.1. XM_003751721.2.
XP_006254204.1. XM_006254142.1.
UniGenei Rn.234505.

3D structure databases

ProteinModelPortali Q498S8.
SMRi Q498S8. Positions 32-153.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

GPCRDBi Search...

PTM databases

PhosphoSitei Q498S8.

Proteomic databases

PaxDbi Q498S8.
PRIDEi Q498S8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000058837 ; ENSRNOP00000055627 ; ENSRNOG00000038571 .
ENSRNOT00000073575 ; ENSRNOP00000067651 ; ENSRNOG00000048236 .
GeneIDi 100909849.
364754.
KEGGi rno:100909849.
rno:364754.
UCSCi RGD:1560525. rat.

Organism-specific databases

CTDi 8325.
RGDi 1560525. Fzd8.

Phylogenomic databases

eggNOGi NOG257258.
GeneTreei ENSGT00750000117488.
HOGENOMi HOG000233236.
HOVERGENi HBG006977.
InParanoidi Q498S8.
KOi K02375.
OMAi HEKVACN.
OrthoDBi EOG7M3J01.
PhylomeDBi Q498S8.
TreeFami TF317907.

Miscellaneous databases

NextBioi 685973.
PROi Q498S8.

Gene expression databases

Genevestigatori Q498S8.

Family and domain databases

Gene3Di 1.10.2000.10. 1 hit.
InterProi IPR000539. Frizzled.
IPR015526. Frizzled/SFRP.
IPR020067. Frizzled_dom.
IPR017981. GPCR_2-like.
[Graphical view ]
PANTHERi PTHR11309. PTHR11309. 1 hit.
Pfami PF01534. Frizzled. 1 hit.
PF01392. Fz. 1 hit.
[Graphical view ]
PRINTSi PR00489. FRIZZLED.
SMARTi SM00063. FRI. 1 hit.
[Graphical view ]
SUPFAMi SSF63501. SSF63501. 1 hit.
PROSITEi PS50038. FZ. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.

Entry informationi

Entry nameiFZD8_RAT
AccessioniPrimary (citable) accession number: Q498S8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: September 13, 2005
Last modified: July 9, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi