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Q498S8 (FZD8_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Frizzled-8

Short name=Fz-8
Gene names
Name:Fzd8
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length684 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Receptor for Wnt proteins. Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalsomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Coreceptor along with RYK of Wnt proteins, such as WNT1 By similarity.

Subunit structure

Component of a Wnt-signaling complex that contains a WNT protein, a FZD protein and LRP5 or LRP6. Interacts directly with LRP5 or LRP6; the interaction is promoted by Wnt-binding and signaling and inhibited by DKK1. Interacts (via the PDZ-binding motif) with GPOC (via its PDZ domain). Interacts with RSPO1 and RSPO3 By similarity.

Subcellular location

Membrane; Multi-pass membrane protein. Golgi apparatus By similarity. Cell membrane; Multi-pass membrane protein By similarity. Note: Colocalizes with GOPC at the Golgi apparatus By similarity.

Domain

Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway By similarity.

The FZ domain is involved in binding with Wnt ligands By similarity.

Post-translational modification

Ubiquitinated by ZNRF3, leading to its degradation by the proteasome By similarity.

Sequence similarities

Belongs to the G-protein coupled receptor Fz/Smo family.

Contains 1 FZ (frizzled) domain.

Ontologies

Keywords
   Biological processWnt signaling pathway
   Cellular componentCell membrane
Golgi apparatus
Membrane
   DomainSignal
Transmembrane
Transmembrane helix
   Molecular functionDevelopmental protein
G-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
Ubl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processT cell differentiation in thymus

Inferred from Biological aspect of Ancestor. Source: RefGenome

axonogenesis

Inferred from Biological aspect of Ancestor. Source: RefGenome

brain development

Inferred from Biological aspect of Ancestor. Source: RefGenome

canonical Wnt signaling pathway

Inferred from Biological aspect of Ancestor. Source: RefGenome

gonad development

Inferred from Biological aspect of Ancestor. Source: RefGenome

vasculature development

Inferred from Biological aspect of Ancestor. Source: RefGenome

   Cellular_componentGolgi apparatus

Inferred from electronic annotation. Source: UniProtKB-SubCell

cell projection

Inferred from Biological aspect of Ancestor. Source: RefGenome

cytoplasm

Inferred from Biological aspect of Ancestor. Source: RefGenome

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from Biological aspect of Ancestor. Source: RefGenome

   Molecular_functionG-protein coupled receptor activity

Inferred from electronic annotation. Source: UniProtKB-KW

PDZ domain binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

Wnt-activated receptor activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

Wnt-protein binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Potential
Chain28 – 684657Frizzled-8
PRO_0000278650

Regions

Topological domain28 – 271244Extracellular Potential
Transmembrane272 – 29221Helical; Name=1; Potential
Topological domain293 – 30816Cytoplasmic Potential
Transmembrane309 – 32921Helical; Name=2; Potential
Topological domain330 – 39364Extracellular Potential
Transmembrane394 – 41421Helical; Name=3; Potential
Topological domain415 – 43622Cytoplasmic Potential
Transmembrane437 – 45721Helical; Name=4; Potential
Topological domain458 – 48023Extracellular Potential
Transmembrane481 – 50121Helical; Name=5; Potential
Topological domain502 – 52928Cytoplasmic Potential
Transmembrane530 – 55021Helical; Name=6; Potential
Topological domain551 – 58131Extracellular Potential
Transmembrane582 – 60221Helical; Name=7; Potential
Topological domain603 – 68482Cytoplasmic Potential
Domain30 – 151122FZ
Region71 – 788Palmitate-binding groove By similarity
Region95 – 1006Wnt-binding By similarity
Region147 – 1526Wnt-binding By similarity
Motif605 – 6106Lys-Thr-X-X-X-Trp motif, mediates interaction with the PDZ domain of Dvl family members By similarity
Motif682 – 6843PDZ-binding By similarity
Compositional bias161 – 22666Pro-rich
Compositional bias168 – 1736Poly-Pro
Compositional bias639 – 65315Poly-Gly

Amino acid modifications

Glycosylation491N-linked (GlcNAc...) Potential
Glycosylation1521N-linked (GlcNAc...) Potential
Glycosylation4721N-linked (GlcNAc...) Potential
Disulfide bond35 ↔ 96 By similarity
Disulfide bond43 ↔ 89 By similarity
Disulfide bond80 ↔ 118 By similarity
Disulfide bond107 ↔ 148 By similarity
Disulfide bond111 ↔ 135 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q498S8 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: EEBA871326749B69

FASTA68473,009
        10         20         30         40         50         60 
MEWGYLLEVT SLLAALAVLQ RSSGAAAASA KELACQEITV PLCKGIGYNY TYMPNQFNHD 

        70         80         90        100        110        120 
TQDEAGLEVH QFWPLVEIQC SPDLKFFLCS MYTPICLEDY KKPLPPCRSV CERAKAGCAP 

       130        140        150        160        170        180 
LMRQYGFAWP DRMRCDRLPE QGNPDTLCMD YNRTDLTTAA PSPPRRLPPP QPGEQPPSGS 

       190        200        210        220        230        240 
GHSRPPGARP PHRGGSSRGS GDTAAAPPSR GGKARPPGGG AAPCEPGCQC RAPMVSVSSE 

       250        260        270        280        290        300 
RHPLYNRVKT GQIANCALPC HNPFFSQDER AFTVFWIGLW SVLCFVSTFA TVSTFLIDME 

       310        320        330        340        350        360 
RFKYPERPII FLSACYLFVS VGYLVRLVAG HEKVACSGGA PGAGGAGGAG GAATAGAGAA 

       370        380        390        400        410        420 
GAGASSPGAR GEYEELGAVE QHVRYETTGP ALCTVVFLLV YFFGMASSIW WVILSLTWFL 

       430        440        450        460        470        480 
AAGMKWGNEA IAGYSQYFHL AAWLVPSVKS IAVLALSSVD GDPVAGICYV GNQSLDNLRG 

       490        500        510        520        530        540 
FVLAPLVIYL FIGTMFLLAG FVSLFRIRSV IKQQGGPTKT HKLEKLMIRL GLFTVLYTVP 

       550        560        570        580        590        600 
AAVVVACLFY EQHNRPRWEA THNCPCLRDL QPDQARRPDY AVFMLKYFMC LVVGITSGVW 

       610        620        630        640        650        660 
VWSGKTLESW RALCTRCCWA SKGAAVGAGA GGSGPGGGGP GPGGGGGHGG GGGSLYSDVS 

       670        680 
TGLTWRSGTA SSVSYPKQMP LSQV 

« Hide

References

[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC100088 mRNA. Translation: AAI00089.1.
RefSeqNP_001037716.1. NM_001044251.2.
XP_003751769.1. XM_003751721.2.
XP_006254204.1. XM_006254142.1.
UniGeneRn.234505.

3D structure databases

ProteinModelPortalQ498S8.
SMRQ498S8. Positions 32-153.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteQ498S8.

Proteomic databases

PaxDbQ498S8.
PRIDEQ498S8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000058837; ENSRNOP00000055627; ENSRNOG00000038571.
ENSRNOT00000073575; ENSRNOP00000067651; ENSRNOG00000048236.
GeneID100909849.
364754.
KEGGrno:100909849.
rno:364754.
UCSCRGD:1560525. rat.

Organism-specific databases

CTD8325.
RGD1560525. Fzd8.

Phylogenomic databases

eggNOGNOG257258.
GeneTreeENSGT00750000117488.
HOGENOMHOG000233236.
HOVERGENHBG006977.
InParanoidQ498S8.
KOK02375.
OMAHEKVACN.
OrthoDBEOG7M3J01.
PhylomeDBQ498S8.
TreeFamTF317907.

Gene expression databases

GenevestigatorQ498S8.

Family and domain databases

Gene3D1.10.2000.10. 1 hit.
InterProIPR000539. Frizzled.
IPR015526. Frizzled/SFRP.
IPR020067. Frizzled_dom.
IPR017981. GPCR_2-like.
[Graphical view]
PANTHERPTHR11309. PTHR11309. 1 hit.
PfamPF01534. Frizzled. 1 hit.
PF01392. Fz. 1 hit.
[Graphical view]
PRINTSPR00489. FRIZZLED.
SMARTSM00063. FRI. 1 hit.
[Graphical view]
SUPFAMSSF63501. SSF63501. 1 hit.
PROSITEPS50038. FZ. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio685973.
PROQ498S8.

Entry information

Entry nameFZD8_RAT
AccessionPrimary (citable) accession number: Q498S8
Entry history
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: September 13, 2005
Last modified: July 9, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries