Reviewed, UniProtKB/Swiss-Prot Q498R3 (DJC10_RAT)

Names and origin

Protein names

Recommended name:
    DnaJ homolog subfamily C member 10

Gene names

Name:

Dnajc10

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	793 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	This endoplasmic reticulum co-chaperone may play a role in protein folding and translocation across the endoplasmic reticulum membrane. May be involved in disulfide formation. May act as a co-chaperone for Hspa5 By similarity.
Subunit structure	Interacts with HSPA5 via its J domain By similarity.
Subcellular location	Secreted Potential. Endoplasmic reticulum lumen By similarity.
Sequence similarities	Contains 1 J domain. Contains 4 thioredoxin domains.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Secreted
Domain	Repeat Signal
Molecular function	Chaperone
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro protein folding Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	heat shock protein binding Inferred from electronic annotation. Source: InterPro unfolded protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 32	32	Potential
	Chain	33 – 793	761	DnaJ homolog subfamily C member 10		PRO_0000281486
Regions
	Domain	35 – 100	66	J
	Domain	130 – 232	103	Thioredoxin 1
	Domain	454 – 553	100	Thioredoxin 2
	Domain	557 – 665	109	Thioredoxin 3
	Domain	671 – 776	106	Thioredoxin 4
	Motif	790 – 793	4	Prevents secretion from ER Potential
Amino acid modifications
	Glycosylation	530	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q498R3-1 [UniParc]. Last modified March 20, 2007. Version 2. Checksum: 9578A69C36C858C9	FASTA	793	90,747
	10 20 30 40 50 60 MGVWLNRDEF IRDVKRISLC LLVLYVVIVV GTDQNFYSLL GVSKTASSRE IRQAFKKLAL 70 80 90 100 110 120 KLHPDKNPNN PNAHGDFLKI NRAYEVLKDE DLRKKYDKYG EKGLEDNQGG QYESWSYYRY 130 140 150 160 170 180 DFGIYDDDPE IITLERREFD AAVNSGELWF VNFYSPGCSH CHDLAPTWRE FAKEVDGLLR 190 200 210 220 230 240 IGAVNCGDDR MLCRMKGVNS YPSLFIFRSG MAAVKYNGDR SKESLVSFAM QHVRTTVTEL 250 260 270 280 290 300 STGNFVNAIE TAFAAGIGWL ITFCFKGEDC LTPQTRLRLS GMLDGLVNVG WVDCDTQDSL 310 320 330 340 350 360 CKSLDATAST TAYFPPGATL NNKEKSSVLF LNSLDAKEIY MEIIHNLPDF ELLSANKLED 370 380 390 400 410 420 RLAHHRWLVF FHFGKNENAN DPELKKLKTL LKNEHIQVGR FDCSSAPGIC SDLYVFQSCL 430 440 450 460 470 480 AVFKGQGTKE YEIHHGKKIL YDILAFAKES VNSHVTTLGP QNFPASDKEP WLVDFFAPWC 490 500 510 520 530 540 PPCRALLPEL RKASTLLYGQ LKVGTLDCTI HEGLCNMYNI QAYPTTVVFN QSSVHEYEGH 550 560 570 580 590 600 HSAEQILEFI EDLRNPSVVS LTPTTFNELV KQRKHDEVWM VDFYSPWCHP CQVLMPEWKR 610 620 630 640 650 660 MARTLTGLIN VGSVDCQQYH SFCTQENVQR YPEIRFYPQK SSRAYQYHSY NGWNRDAYSL 670 680 690 700 710 720 RSWGLGFLPQ ASIDLTPQTF NEKVLQGKTH WVIDFYAPWC GPCQNFAPEF ELLARMIKGK 730 740 750 760 770 780 VKAGKVDCQA YPQTCQKAGI RAYPSVKLYL YERAKKSIWE EQINSRDAKT IAALIYGKLE 790 TFQSQVKRNK DEL

References

[1]

"Genome sequence of the Brown Norway rat yields insights into mammalian evolution." Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., Morgan M. , Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S., Collins F.S. Nature 428:493-521(2004) [PubMed: 15057822] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Brown Norway.

[2]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 262-793. Tissue: Spleen.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AABR03024291 Genomic DNA. No translation available. BC100105 mRNA. Translation: AAI00106.1.
IPI	IPI00372776.
RefSeq	NP_001099956.2.
UniGene	Rn.8642
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q498R3.
Genome annotation databases
Ensembl	ENSRNOG00000006803. Rattus norvegicus. [Contig view]
GeneID	295690.
KEGG	rno:295690.
Organism-specific databases
RGD	1307813. Dnajc10.
Phylogenomic databases
HOVERGEN	Q498R3.
Family and domain databases
InterPro	IPR001623. DnaJ_N. IPR000886. ER_targeting_sequence. IPR018253. Heat_shock_DnaJ_CS. IPR015609. Hsp40/DnaJ_Rel. IPR003095. Hsp_DnaJ. IPR017936. Thioredoxin-like. IPR017937. Thioredoxin_CS. IPR013766. Thioredoxin_domain. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:1.10.287.110. DnaJ_N. 1 hit. G3DSA:3.40.30.10. Thioredoxin_fold. 4 hits.
PANTHER	PTHR11821. Hsp40/DnaJ_Rel. 1 hit.
Pfam	PF00226. DnaJ. 1 hit. PF00085. Thioredoxin. 4 hits. [Graphical view]
PRINTS	PR00625. DNAJPROTEIN.
SMART	SM00271. DnaJ. 1 hit. [Graphical view]
PROSITE	PS00636. DNAJ_1. False negative. PS50076. DNAJ_2. 1 hit. PS00014. ER_TARGET. 1 hit. PS00194. THIOREDOXIN_1. 2 hits. PS51352. THIOREDOXIN_2. 3 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	639891.

Entry information

Entry name

DJC10_RAT

Accession

Primary (citable) accession number: Q498R3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 2007
Last sequence update:	March 20, 2007
Last modified:	June 16, 2009
This is version 47 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)