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Protein

Methionine synthase reductase

Gene

Mtrr

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the reductive regeneration of cob(I)alamin (vitamin B12) cofactor required for the maintenance of methionine synthase in a functional state. Necessary for utilization of methylgroups from the folate cycle, thereby affecting transgenerational epigenetic inheritance. Folate pathway donates methyl groups necessary for cellular methylation and affects different pathways such as DNA methylation, possibly explaining the transgenerational epigenetic inheritance effects (By similarity).By similarity

Catalytic activityi

2 [methionine synthase]-methylcob(I)alamin + 2 S-adenosylhomocysteine + NADP+ = 2 [methionine synthase]-cob(II)alamin + NADPH + 2 S-adenosyl-L-methionine.

Cofactori

Protein has several cofactor binding sites:
  • FADBy similarity
  • FMNBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei292NADPBy similarity1
Binding sitei661NADPBy similarity1
Binding sitei699FADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 14FMNPROSITE-ProRule annotation5
Nucleotide bindingi93 – 124FMNPROSITE-ProRule annotationAdd BLAST32
Nucleotide bindingi452 – 455FADBy similarity4
Nucleotide bindingi488 – 491FADBy similarity4
Nucleotide bindingi611 – 612NADPBy similarity2
Nucleotide bindingi626 – 628NADPBy similarity3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

FAD, Flavoprotein, FMN, NADP, S-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-RNO-156581. Methylation.
R-RNO-1614635. Sulfur amino acid metabolism.
R-RNO-196741. Cobalamin (Cbl, vitamin B12) transport and metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine synthase reductase (EC:1.16.1.8)
Short name:
MSR
Gene namesi
Name:Mtrr
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi1308671. Mtrr.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004093091 – 700Methionine synthase reductaseAdd BLAST700

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei173PhosphoserineBy similarity1
Modified residuei190PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ498R1.
PRIDEiQ498R1.

Expressioni

Gene expression databases

BgeeiENSRNOG00000017826.
GenevisibleiQ498R1. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024041.

Structurei

3D structure databases

ProteinModelPortaliQ498R1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 147Flavodoxin-likePROSITE-ProRule annotationAdd BLAST144
Domaini272 – 534FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST263

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni168 – 247HingeBy similarityAdd BLAST80

Sequence similaritiesi

Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
Contains 1 flavodoxin-like domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1158. Eukaryota.
COG0369. LUCA.
GeneTreeiENSGT00840000129757.
HOGENOMiHOG000007485.
HOVERGENiHBG108376.
InParanoidiQ498R1.
KOiK00597.
OMAiFSVICPN.
OrthoDBiEOG091G045Q.
TreeFamiTF105716.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like_dom.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q498R1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRRFLLLYAT QRGQAKAIAE EISEQALSHG FSADLHCVSE SEKYDLKTET
60 70 80 90 100
GPLVMVVSTT GTGDPPDTAR KFVKEIHNKT LPTDFFAHLW YGLLGLGDSE
110 120 130 140 150
YTYFCNGGKV IDKRLQELGA QHFYDTGHAD DCVGLELVVE PWIDGLWPAL
160 170 180 190 200
TKHFKSLGGQ EDMSDSDTLA QASDAPLSMA MKPELLHIQS QVELLSLEDV
210 220 230 240 250
GKRDSELQEQ NETNKNQPSR IEDFDSSLVN SVPPLSQSSL SIPAVSPEYL
260 270 280 290 300
EVYLQESLGQ DENQASVPPS VDPIFQVPIS KAVELTTNDA IKTTLLLELD
310 320 330 340 350
ISKVEFSHQP GDSFNVICPN SGSEVEDLLQ RLQLADKQAH RVILKIKMDT
360 370 380 390 400
KKKGASLPQH VPEGSSLQFI FTWCLEIRAV PKKAFLRALS DYTSDATEKR
410 420 430 440 450
RLQELCSKQG AADYNRFIRD ASVCLLDLLL TFPSCQPPLN LLLEHLPKLQ
460 470 480 490 500
PRPYSCASSS LLHPDKLHFV FNIVELPSNT TAASLRKGVC TGWLATLVAP
510 520 530 540 550
FLQPNTEVLT ADHSDALAPE ILISPRATNS FHLPDDLSAP IIMVGPGTGV
560 570 580 590 600
APFVGFLQHR EKLQEQHPDG NFGAMWLFFG CRHKDRDYLF REELRHFLKT
610 620 630 640 650
GVLTHLKVSF SRDAAPEEEE EAPAKYVQDN LQHHSQQVAR TLLQENGYIY
660 670 680 690 700
VCGDAKNMAK DVHDALVEII SKEAGVDKLE AMKTLATLKQ EKRYLQDIWS
Length:700
Mass (Da):78,025
Last modified:May 31, 2011 - v2
Checksum:i13EAE5AE46606F11
GO
Isoform 2 (identifier: Q498R1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     44-237: Missing.

Show »
Length:506
Mass (Da):56,531
Checksum:iFAA82D70082C004C
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04129444 – 237Missing in isoform 2. 1 PublicationAdd BLAST194

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH474002 Genomic DNA. Translation: EDL87600.1.
BC100107 mRNA. Translation: AAI00108.1.
RefSeqiNP_001034092.1. NM_001039003.1. [Q498R1-2]
XP_006227875.1. XM_006227813.3. [Q498R1-1]
UniGeneiRn.229885.

Genome annotation databases

EnsembliENSRNOT00000024041; ENSRNOP00000024041; ENSRNOG00000017826. [Q498R1-1]
ENSRNOT00000061790; ENSRNOP00000058505; ENSRNOG00000017826. [Q498R1-2]
GeneIDi290947.
KEGGirno:290947.
UCSCiRGD:1308671. rat. [Q498R1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH474002 Genomic DNA. Translation: EDL87600.1.
BC100107 mRNA. Translation: AAI00108.1.
RefSeqiNP_001034092.1. NM_001039003.1. [Q498R1-2]
XP_006227875.1. XM_006227813.3. [Q498R1-1]
UniGeneiRn.229885.

3D structure databases

ProteinModelPortaliQ498R1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024041.

Proteomic databases

PaxDbiQ498R1.
PRIDEiQ498R1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000024041; ENSRNOP00000024041; ENSRNOG00000017826. [Q498R1-1]
ENSRNOT00000061790; ENSRNOP00000058505; ENSRNOG00000017826. [Q498R1-2]
GeneIDi290947.
KEGGirno:290947.
UCSCiRGD:1308671. rat. [Q498R1-1]

Organism-specific databases

CTDi4552.
RGDi1308671. Mtrr.

Phylogenomic databases

eggNOGiKOG1158. Eukaryota.
COG0369. LUCA.
GeneTreeiENSGT00840000129757.
HOGENOMiHOG000007485.
HOVERGENiHBG108376.
InParanoidiQ498R1.
KOiK00597.
OMAiFSVICPN.
OrthoDBiEOG091G045Q.
TreeFamiTF105716.

Enzyme and pathway databases

ReactomeiR-RNO-156581. Methylation.
R-RNO-1614635. Sulfur amino acid metabolism.
R-RNO-196741. Cobalamin (Cbl, vitamin B12) transport and metabolism.

Miscellaneous databases

PROiQ498R1.

Gene expression databases

BgeeiENSRNOG00000017826.
GenevisibleiQ498R1. RN.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like_dom.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMTRR_RAT
AccessioniPrimary (citable) accession number: Q498R1
Secondary accession number(s): D3ZDG8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 31, 2011
Last sequence update: May 31, 2011
Last modified: November 2, 2016
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.