Q498M4 (WDR5_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
WD repeat-containing protein 5

Gene names

Name:

Wdr5

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Contributes to histone modification. May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4'. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. May regulate osteoblasts differentiation By similarity.
Subunit structure	Interacts with HCFC1 By similarity. Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4 By similarity. Component of the SET1 complex, at least composed of the catalytic subunit (SETD1A or SETD1B), WDR5, WDR82, RBBP5, ASH2L/ASH2, CXXC1/CFP1, HCFC1 and DPY30 By similarity. Core component of several methyltransferase-containing complexes including MLL1/MLL, MLL2/3 (also named ASCOM complex) and MLL4/WBP7. Each complex is at least composed of ASH2L, RBBP5, WDR5, DPY30, one or more specific histone methyltransferases (MLL, MLL2/ALR, MLL3 and MLL4/WBP7), and the facultative components PAGR1, BAP18, CHD8, E2F6, HCFC1, HCFC2, HSP70, INO80C, KDM6A, KANSL1, LAS1L, MAX, MCRS1, MEN1, MGA, MYST1/MOF, NCOA6, PAXIP1/PTIP, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9, TEX10 and alpha- and beta-tubulin By similarity. Component of the NSL complex at least composed of MOF/KAT8, KANSL1, KANSL2, KANSL3, MCRS1, PHF20, OGT1/OGT, WDR5 and HCFC1 By similarity. Interacts with MLL and RBBP5; the interaction is direct By similarity. Component of the ADA2A-containing complex (ATAC), composed of CSRP2BP, KAT2A, TADA2L, TADA3L, ZZ3, MBIP, WDR5, YEATS2, CCDC101 and DR1. In the complex, it probably interacts directly with KAT2A, MBIP and CSRP2BP By similarity. Interacts with histone H3 By similarity. Interacts with SETD1A By similarity. Interacts with PAXBP1; the interaction is direct and links a WDR5-containing histone methyltransferase complex to PAX7 and PAX3 By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the WD repeat WDR5/wds family. Contains 7 WD repeats.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat WD repeat
Molecular function	Chromatin regulator
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	histone H3 acetylation Inferred from electronic annotation. Source: Compara histone H3-K4 methylation Inferred from sequence or structural similarity. Source: UniProtKB histone H4-K16 acetylation Inferred from sequence or structural similarity. Source: UniProtKB histone H4-K5 acetylation Inferred from sequence or structural similarity. Source: UniProtKB histone H4-K8 acetylation Inferred from sequence or structural similarity. Source: UniProtKB regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW skeletal system development Inferred from electronic annotation. Source: Compara transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Ada2/Gcn5/Ada3 transcription activator complex Inferred from electronic annotation. Source: Compara MLL1 complex Inferred from sequence or structural similarity. Source: UniProtKB Set1C/COMPASS complex Inferred from sequence or structural similarity. Source: UniProtKB histone acetyltransferase complex Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	histone acetyltransferase activity (H4-K16 specific) Inferred from electronic annotation. Source: Compara histone acetyltransferase activity (H4-K5 specific) Inferred from electronic annotation. Source: Compara histone acetyltransferase activity (H4-K8 specific) Inferred from electronic annotation. Source: Compara histone methyltransferase activity (H3-K4 specific) Inferred from electronic annotation. Source: Compara methylated histone residue binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 334	334	WD repeat-containing protein 5		PRO_0000278192
Regions
	Repeat	43 – 82	40	WD 1
	Repeat	85 – 126	42	WD 2
	Repeat	128 – 168	41	WD 3
	Repeat	169 – 208	40	WD 4
	Repeat	212 – 253	42	WD 5
	Repeat	256 – 296	41	WD 6
	Repeat	299 – 333	35	WD 7
Sites
	Site	107	1	Important for interaction with histone H3 By similarity
	Site	133	1	Important for interaction with histone H3 By similarity
	Site	263	1	Important for interaction with histone H3 By similarity
	Site	322	1	Important for interaction with histone H3 By similarity
Amino acid modifications
	Modified residue	112	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q498M4 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 4BF30914A2250286	FASTA	334	36,588
	10 20 30 40 50 60 MATEEKKPET EAARAQPTPS SSATQSKPTP VKPNYALKFT LAGHTKAVSS VKFSPNGEWL 70 80 90 100 110 120 ASSSADKLIK IWGAYDGKFE KTISGHKLGI SDVAWSSDSN LLVSASDDKT LKIWDVSSGK 130 140 150 160 170 180 CLKTLKGHSN YVFCCNFNPQ SNLIVSGSFD ESVRIWDVKT GKCLKTLPAH SDPVSAVHFN 190 200 210 220 230 240 RDGSLIVSSS YDGLCRIWDT ASGQCLKTLI DDDNPPVSFV KFSPNGKYIL AATLDNTLKL 250 260 270 280 290 300 WDYSKGKCLK TYTGHKNEKY CIFANFSVTG GKWIVSGSED NLVYIWNLQT KEIVQKLQGH 310 320 330 TDVVISTACH PTENIIASAA LENDKTIKLW KSDC

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC100156 mRNA. Translation: AAI00157.1.
IPI	IPI00373481.
RefSeq	NP_001034123.1. NM_001039034.1.
UniGene	Rn.106818.
3D structure databases
HSSP	HSSP built from PDB template 2GNQ based on UniProtKB P61964.
ProteinModelPortal	Q498M4.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q498M4. 1 interaction.
STRING	10116.ENSRNOP00000011556.
Proteomic databases
PaxDb	Q498M4.
PRIDE	Q498M4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000011556; ENSRNOP00000011556; ENSRNOG00000008212.
GeneID	362093.
KEGG	rno:362093.
UCSC	RGD:1305159. rat.
Organism-specific databases
CTD	11091.
RGD	1305159. Wdr5.
Phylogenomic databases
eggNOG	COG2319.
GeneTree	ENSGT00690000101787.
HOGENOM	HOG000091642.
HOVERGEN	HBG055117.
InParanoid	Q498M4.
KO	K14963.
OMA	KLWKSDT.
OrthoDB	EOG4QVCCF.
Gene expression databases
Genevestigator	Q498M4.
Family and domain databases
Gene3D	2.130.10.10. 2 hits.
InterPro	IPR020472. G-protein_beta_WD-40_rep. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_dom. [Graphical view]
Pfam	PF00400. WD40. 7 hits. [Graphical view]
PRINTS	PR00320. GPROTEINBRPT.
SMART	SM00320. WD40. 7 hits. [Graphical view]
SUPFAM	SSF50978. WD40_like. 1 hit.
PROSITE	PS00678. WD_REPEATS_1. 4 hits. PS50082. WD_REPEATS_2. 6 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q498M4.
NextBio	678628.

Entry information

Entry name

WDR5_RAT

Accession

Primary (citable) accession number: Q498M4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	September 13, 2005
Last modified:	May 1, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program