Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase

Gene

miaB

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine.UniRule annotation

Catalytic activityi

N(6)-dimethylallyladenine(37) in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + reduced electron acceptor = 2-methylthio-N(6)-dimethylallyladenine(37) in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + electron acceptor.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi27 – 271Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi66 – 661Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi100 – 1001Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi174 – 1741Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi178 – 1781Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi181 – 1811Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseUniRule annotation (EC:2.8.4.3UniRule annotation)
Alternative name(s):
(Dimethylallyl)adenosine tRNA methylthiotransferase MiaBUniRule annotation
tRNA-i(6)A37 methylthiotransferaseUniRule annotation
Gene namesi
Name:miaBUniRule annotation
Ordered Locus Names:ML0989
ORF Names:B2235_C2_195
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000806 Componenti: Chromosome

Organism-specific databases

LepromaiML0989.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 517517tRNA-2-methylthio-N(6)-dimethylallyladenosine synthasePRO_0000141744Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi272631.ML0989.

Structurei

3D structure databases

ProteinModelPortaliQ49842.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini18 – 137120MTTase N-terminalUniRule annotationAdd
BLAST
Domaini399 – 47072TRAMUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the methylthiotransferase family. MiaB subfamily.UniRule annotation
Contains 1 MTTase N-terminal domain.UniRule annotation
Contains 1 TRAM domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0621. LUCA.
HOGENOMiHOG000224767.
KOiK06168.
OMAiCQMNFSD.
OrthoDBiEOG6P5ZD8.

Family and domain databases

Gene3Di3.80.30.20. 1 hit.
HAMAPiMF_01864. tRNA_metthiotr_MiaB.
InterProiIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERiPTHR11918. PTHR11918. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00089. TIGR00089. 1 hit.
PROSITEiPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q49842-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTRDAGMAD DAATGSTRTY QVRTYGCQMN VHDSERLAGL LEAAGYQRAA
60 70 80 90 100
DEADVGDADV VVFNTCAVRE NADNRLYGNL SHLAPRKRNN PDMQIAVGGC
110 120 130 140 150
LAQKDKHTVL SKAPWVDIVF GTHNLGSLPT LLDRARHNKV AQVEIVEALQ
160 170 180 190 200
HFPSSLPSAR ESDYAAWVSI SVGCNNSCTF CIVPSLRGKE VDRSPADILA
210 220 230 240 250
EVEALVADGV LEVTLLGQNV NAYGVSFADP ALARNRGSFA ELLRSCGSID
260 270 280 290 300
GLERVRFTSP HPAEFTYDVI EAMAQTPNVC PSLHMPLQSG SDRVLRAMRR
310 320 330 340 350
SYRVERYLGI IGQVRAAMPH AAITTDLIVG FPGETEEDFA ATLDVVRQVR
360 370 380 390 400
FTAAFTFQYS KRPGTPAAEL GGQLPKAVVQ ERYERLVELQ EQICMEENRV
410 420 430 440 450
LIGRIVELLV TTGEGRKDAR TARRTGRARD GRLVHFALDT PWEAQVRPGD
460 470 480 490 500
IITVMVTEAA PHHLIADAGI LTHRRTRAGD AHAARQCPRS IGLGQIPLGM
510
PSVGQHSASI GSAGCAR
Length:517
Mass (Da):56,123
Last modified:April 27, 2001 - v2
Checksum:i1D3F4336857B479E
GO

Sequence cautioni

The sequence AAA17273.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00019 Genomic DNA. Translation: AAA17273.1. Different initiation.
AL583920 Genomic DNA. Translation: CAC31370.1.
PIRiG87032.
S72937.
RefSeqiNP_301734.1. NC_002677.1.
WP_010908058.1. NC_002677.1.

Genome annotation databases

EnsemblBacteriaiCAC31370; CAC31370; CAC31370.
GeneIDi910281.
KEGGimle:ML0989.
PATRICi18053692. VBIMycLep78757_1791.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00019 Genomic DNA. Translation: AAA17273.1. Different initiation.
AL583920 Genomic DNA. Translation: CAC31370.1.
PIRiG87032.
S72937.
RefSeqiNP_301734.1. NC_002677.1.
WP_010908058.1. NC_002677.1.

3D structure databases

ProteinModelPortaliQ49842.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272631.ML0989.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC31370; CAC31370; CAC31370.
GeneIDi910281.
KEGGimle:ML0989.
PATRICi18053692. VBIMycLep78757_1791.

Organism-specific databases

LepromaiML0989.

Phylogenomic databases

eggNOGiCOG0621. LUCA.
HOGENOMiHOG000224767.
KOiK06168.
OMAiCQMNFSD.
OrthoDBiEOG6P5ZD8.

Family and domain databases

Gene3Di3.80.30.20. 1 hit.
HAMAPiMF_01864. tRNA_metthiotr_MiaB.
InterProiIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERiPTHR11918. PTHR11918. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00089. TIGR00089. 1 hit.
PROSITEiPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Smith D.R., Robison K.
    Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TN.

Entry informationi

Entry nameiMIAB_MYCLE
AccessioniPrimary (citable) accession number: Q49842
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: April 27, 2001
Last modified: November 11, 2015
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.