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Protein

Porphobilinogen deaminase

Gene

hemC

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.By similarity

Catalytic activityi

4 porphobilinogen + H2O = hydroxymethylbilane + 4 NH3.

Cofactori

dipyrromethaneBy similarityNote: Binds 1 dipyrromethane group covalently.By similarity

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Delta-aminolevulinic acid dehydratase (hemB)
  2. Porphobilinogen deaminase (hemC)
  3. no protein annotated in this organism
  4. Uroporphyrinogen decarboxylase (hemE)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Porphyrin biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00251; UER00319.

Names & Taxonomyi

Protein namesi
Recommended name:
Porphobilinogen deaminase (EC:2.5.1.61)
Short name:
PBG
Alternative name(s):
Hydroxymethylbilane synthase
Short name:
HMBS
Pre-uroporphyrinogen synthase
Gene namesi
Name:hemC
Ordered Locus Names:ML2421
ORF Names:B2168_C1_179, B2168_C3_262
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000806 Componenti: Chromosome

Organism-specific databases

LepromaiML2421.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 315315Porphobilinogen deaminasePRO_0000142958Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei234 – 2341S-(dipyrrolylmethanemethyl)cysteineBy similarity

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi272631.ML2421.

Structurei

3D structure databases

ProteinModelPortaliQ49808.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HMBS family.Curated

Phylogenomic databases

eggNOGiENOG4105D6W. Bacteria.
COG0181. LUCA.
HOGENOMiHOG000228588.
OrthoDBiPOG091H064B.

Family and domain databases

Gene3Di3.30.160.40. 1 hit.
HAMAPiMF_00260. Porphobil_deam. 1 hit.
InterProiIPR000860. HemC.
IPR022419. Porphobilin_deaminase_cofac_BS.
IPR022417. Porphobilin_deaminase_N.
IPR022418. Porphobilinogen_deaminase_C.
[Graphical view]
PANTHERiPTHR11557. PTHR11557. 1 hit.
PfamiPF01379. Porphobil_deam. 1 hit.
PF03900. Porphobil_deamC. 1 hit.
[Graphical view]
PIRSFiPIRSF001438. 4pyrrol_synth_OHMeBilane_synth. 1 hit.
PRINTSiPR00151. PORPHBDMNASE.
SUPFAMiSSF54782. SSF54782. 1 hit.
TIGRFAMsiTIGR00212. hemC. 1 hit.
PROSITEiPS00533. PORPHOBILINOGEN_DEAM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q49808-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIRIGTRGSL LATTQAALVR DALIANGHPA ELVIVNTAGD QSSASIDSLG
60 70 80 90 100
VGVFTTALRA AIEEGCVDAA VHSYKDLPTA DDPRFTVAAI PPRNDPRDAV
110 120 130 140 150
VTRDELVLAE LPAGSLVGTS SPRRAAQLRA LGLGLEIRPL RGNLDTRLNR
160 170 180 190 200
VSSGDLDAIV VARAGLARLG RLDEVTETLD PVQMVPAPAQ GAIAVECRAG
210 220 230 240 250
DSRLVAVLAA LDDADTRAAV TAERVLLAEL EAGCSAPVGA IAQVVESIDG
260 270 280 290 300
EGRVFEELSL RGCVAALDGS DVIRASGIST SGRASELGLA VAVELFELGA
310
RELMWGARSD RARGS
Length:315
Mass (Da):32,523
Last modified:April 27, 2001 - v2
Checksum:i64685F2D970D3882
GO

Sequence cautioni

The sequence AAA17244 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAC31937 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti169 – 1691L → P in AAA17223 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00018 Genomic DNA. Translation: AAA17223.1.
U00018 Genomic DNA. Translation: AAA17244.1. Different initiation.
AL583925 Genomic DNA. Translation: CAC31937.1. Different initiation.
PIRiA87212.
S72887.
S72908.
RefSeqiWP_010908886.1. NC_002677.1.

Genome annotation databases

EnsemblBacteriaiCAC31937; CAC31937; CAC31937.
PATRICi18059487. VBIMycLep78757_4658.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00018 Genomic DNA. Translation: AAA17223.1.
U00018 Genomic DNA. Translation: AAA17244.1. Different initiation.
AL583925 Genomic DNA. Translation: CAC31937.1. Different initiation.
PIRiA87212.
S72887.
S72908.
RefSeqiWP_010908886.1. NC_002677.1.

3D structure databases

ProteinModelPortaliQ49808.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272631.ML2421.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC31937; CAC31937; CAC31937.
PATRICi18059487. VBIMycLep78757_4658.

Organism-specific databases

LepromaiML2421.

Phylogenomic databases

eggNOGiENOG4105D6W. Bacteria.
COG0181. LUCA.
HOGENOMiHOG000228588.
OrthoDBiPOG091H064B.

Enzyme and pathway databases

UniPathwayiUPA00251; UER00319.

Family and domain databases

Gene3Di3.30.160.40. 1 hit.
HAMAPiMF_00260. Porphobil_deam. 1 hit.
InterProiIPR000860. HemC.
IPR022419. Porphobilin_deaminase_cofac_BS.
IPR022417. Porphobilin_deaminase_N.
IPR022418. Porphobilinogen_deaminase_C.
[Graphical view]
PANTHERiPTHR11557. PTHR11557. 1 hit.
PfamiPF01379. Porphobil_deam. 1 hit.
PF03900. Porphobil_deamC. 1 hit.
[Graphical view]
PIRSFiPIRSF001438. 4pyrrol_synth_OHMeBilane_synth. 1 hit.
PRINTSiPR00151. PORPHBDMNASE.
SUPFAMiSSF54782. SSF54782. 1 hit.
TIGRFAMsiTIGR00212. hemC. 1 hit.
PROSITEiPS00533. PORPHOBILINOGEN_DEAM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHEM3_MYCLE
AccessioniPrimary (citable) accession number: Q49808
Secondary accession number(s): Q49818, Q9CB59
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: April 27, 2001
Last modified: September 7, 2016
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The porphobilinogen subunits are added to the dipyrromethane group.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.