Reviewed, UniProtKB/Swiss-Prot Q49807 (CFA2_MYCLE)

Names and origin

Protein names

Recommended name:
    Cyclopropane-fatty-acyl-phospholipid synthase
      Short name=Cyclopropane fatty acid synthase
      Short name=CFA synthase
    EC=2.1.1.79
Alternative name(s):
    Cyclopropane mycolic acid synthase

Gene names

Name:	cmaA2
Ordered Locus Names:	ML2426
ORF Names:	B2168_F3_130

Organism

Mycobacterium leprae [Complete proteome] [HAMAP]

Taxonomic identifier

1769 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Transfers a methylene group from S-adenosyl-L-methionine to the cis double bond of an unsaturated fatty acid chain resulting in the replacement of the double bond with a methylene bridge. Mycolic acids, which represent the major constituent of mycobacterial cell wall complex, act as substrates By similarity.
Catalytic activity	S-adenosyl-L-methionine + phospholipid olefinic fatty acid = S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid.
Subcellular location	Cytoplasm By similarity.

Ontologies

Keywords
Biological process	Lipid synthesis
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cyclopropane-fatty-acyl-phospholipid synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	Cyclopropane-fatty-acyl-phospholipid synthase		PRO_0000089568
Regions
	Region	44 – 45	2	S-adenosyl-L-methionine binding By similarity
	Region	79 – 87	9	S-adenosyl-L-methionine binding By similarity
	Region	105 – 110	6	S-adenosyl-L-methionine binding By similarity
Sites
	Site	290	1	Essential for catalysis Probable

Sequences

	Sequence		Length	Mass (Da)
	Q49807-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 9068380ED6D324AA	FASTA	308	35,132
	10 20 30 40 50 60 MVPSQSHPAK TPRKQLKPPI EAVQSHYDRS NEFFKLWLDP SMTYSCAYFE RPDLTLEEAQ 70 80 90 100 110 120 RAKRDLALSK LGLEPGMTLL DIGCGWGSTM LHAIEKYDVN VIGLTLSANQ LAHNKLKFAE 130 140 150 160 170 180 IDHTRTDRTK DVRLQGWEQF DEPVDRIISL GAFEHFADGA GDAGFERYDS FFKMCYDVLP 190 200 210 220 230 240 DDGRMLLHTI IVPDAKETKE LGLTTPMSLL RFIKFILTEI FPGGRLPKIS QVDHYSSNAG 250 260 270 280 290 300 FTVERYHRIG SHYVPTLNAW AAALEAHKDE AIALQGRQIY DTYMHYLTGC SDLFRDRYTD VCQFTLVK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Smith D.R., Robison K. Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Massive gene decay in the leprosy bacillus." Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S. , Feltwell T., Fraser A., Hamlin N., Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S., Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R., Barrell B.G. Nature 409:1007-1011(2001) [PubMed: 11234002] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TN.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U00018 Genomic DNA. Translation: AAA17222.1. AL583925 Genomic DNA. Translation: CAC31942.1.
PIR	S72886.
RefSeq	NP_302570.1.
3D structure databases
HSSP	HSSP built from PDB template 1KPI based on UniProtKB Q11196.
SMR	Q49807. Positions 15-308.
ModBase	Search...
Genome annotation databases
GeneID	908573.
GenomeReviews	Gene locus ML2426 in contig AL450380_GR.
KEGG	mle:ML2426.
NMPDR	fig|272631.1.peg.1442.
Organism-specific databases
Leproma	ML2426.
CMR	Search...
Phylogenomic databases
HOGENOM	Q49807.
OMA	Q49807. RYHRIGS.
Enzyme and pathway databases
BioCyc	MLEP272631:ML2426-MON.
BRENDA	2.1.1.79. 808.
Family and domain databases
InterPro	IPR003333. CMAS. [Graphical view]
Pfam	PF02353. CMAS. 1 hit. [Graphical view]
PIRSF	PIRSF003085. CMAS. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CFA2_MYCLE

Accession

Primary (citable) accession number: Q49807

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2001
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)