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Protein

Omega-amidase NIT2

Gene

Nit2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively.2 Publications

Catalytic activityi

A monoamide of a dicarboxylate + H2O = a dicarboxylate + NH3.1 Publication

Kineticsi

In solution, alpha-ketoglutaramate is in equilibrium with a cyclic form (2-hydroxy-5-oxoproline), and at pH 8.0 or above, the rate of ring opening is no longer limiting for the omega-amidase reaction.

  1. KM=0.0054 mM for alpha-ketoglutaramate (open-chain form)1 Publication
  1. Vmax=20.6 µmol/min/mg enzyme with alpha-ketoglutaramate as substrate1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei43 – 431Proton acceptorPROSITE-ProRule annotation
Active sitei112 – 1121Proton donorPROSITE-ProRule annotation
Active sitei153 – 1531NucleophilePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Omega-amidase NIT2 (EC:3.5.1.31 Publication)
Alternative name(s):
Nitrilase homolog 2
Gene namesi
Name:Nit2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 11

Organism-specific databases

RGDi1310494. Nit2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 276276Omega-amidase NIT2PRO_0000320256Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei26 – 261PhosphoserineBy similarity
Modified residuei68 – 681N6-acetyllysine; alternateBy similarity
Modified residuei68 – 681N6-succinyllysine; alternateBy similarity
Modified residuei123 – 1231N6-succinyllysineBy similarity
Modified residuei130 – 1301N6-succinyllysineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ497B0.
PRIDEiQ497B0.

PTM databases

iPTMnetiQ497B0.
PhosphoSiteiQ497B0.

Expressioni

Gene expression databases

GenevisibleiQ497B0. RN.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000034144.

Structurei

3D structure databases

ProteinModelPortaliQ497B0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 270267CN hydrolasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 CN hydrolase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0806. Eukaryota.
COG0388. LUCA.
GeneTreeiENSGT00550000074838.
HOGENOMiHOG000222700.
HOVERGENiHBG105126.
InParanoidiQ497B0.
KOiK13566.
OMAiNPWGEVI.
OrthoDBiEOG7XDBGD.
PhylomeDBiQ497B0.
TreeFamiTF300747.

Family and domain databases

Gene3Di3.60.110.10. 1 hit.
InterProiIPR003010. C-N_Hydrolase.
[Graphical view]
PfamiPF00795. CN_hydrolase. 1 hit.
[Graphical view]
SUPFAMiSSF56317. SSF56317. 1 hit.
PROSITEiPS50263. CN_HYDROLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q497B0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTFRLALIQ LQVSSIKSDN ITRACSLVRE AAKQGANIVS LPECFNSPYG
60 70 80 90 100
TNYFPEYAEK IPGESTKKLS EVAKENSIYL IGGSIPEEDD GKLYNTCAVF
110 120 130 140 150
GPDGNLLVKH RKIHLFDIDV PGKITFQESK TLSPGDSFST FDTPYCRVGL
160 170 180 190 200
GICYDMRFAE LAQIYARRGC QLLVYPGAFN MTTGPAHWEL LQRARAVDNQ
210 220 230 240 250
VYVATASPAR DEKASYVAWG HSTVVDPWGQ VLTKAGTEET ILYSDIDLKK
260 270
LSEIRQQIPI LKQKRADLYS VESKKP
Length:276
Mass (Da):30,701
Last modified:September 13, 2005 - v1
Checksum:i38C64D674B171EF3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC100637 mRNA. Translation: AAI00638.1.
RefSeqiNP_001029298.1. NM_001034126.1.
UniGeneiRn.42859.

Genome annotation databases

EnsembliENSRNOT00000029420; ENSRNOP00000034144; ENSRNOG00000027797.
GeneIDi288174.
KEGGirno:288174.
UCSCiRGD:1310494. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC100637 mRNA. Translation: AAI00638.1.
RefSeqiNP_001029298.1. NM_001034126.1.
UniGeneiRn.42859.

3D structure databases

ProteinModelPortaliQ497B0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000034144.

PTM databases

iPTMnetiQ497B0.
PhosphoSiteiQ497B0.

Proteomic databases

PaxDbiQ497B0.
PRIDEiQ497B0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000029420; ENSRNOP00000034144; ENSRNOG00000027797.
GeneIDi288174.
KEGGirno:288174.
UCSCiRGD:1310494. rat.

Organism-specific databases

CTDi56954.
RGDi1310494. Nit2.

Phylogenomic databases

eggNOGiKOG0806. Eukaryota.
COG0388. LUCA.
GeneTreeiENSGT00550000074838.
HOGENOMiHOG000222700.
HOVERGENiHBG105126.
InParanoidiQ497B0.
KOiK13566.
OMAiNPWGEVI.
OrthoDBiEOG7XDBGD.
PhylomeDBiQ497B0.
TreeFamiTF300747.

Miscellaneous databases

PROiQ497B0.

Gene expression databases

GenevisibleiQ497B0. RN.

Family and domain databases

Gene3Di3.60.110.10. 1 hit.
InterProiIPR003010. C-N_Hydrolase.
[Graphical view]
PfamiPF00795. CN_hydrolase. 1 hit.
[Graphical view]
SUPFAMiSSF56317. SSF56317. 1 hit.
PROSITEiPS50263. CN_HYDROLASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Prostate.
  2. "Assay and purification of omega-amidase/Nit2, a ubiquitously expressed putative tumor suppressor, that catalyzes the deamidation of the alpha-keto acid analogues of glutamine and asparagine."
    Krasnikov B.F., Nostramo R., Pinto J.T., Cooper A.J.L.
    Anal. Biochem. 391:144-150(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  3. "Identification of the putative tumor suppressor Nit2 as omega-amidase, an enzyme metabolically linked to glutamine and asparagine transamination."
    Krasnikov B.F., Chien C.-H., Nostramo R., Pinto J.T., Nieves E., Callaway M., Sun J., Huebner K., Cooper A.J.L.
    Biochimie 91:1072-1080(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiNIT2_RAT
AccessioniPrimary (citable) accession number: Q497B0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: September 13, 2005
Last modified: June 8, 2016
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.