Reviewed, UniProtKB/Swiss-Prot Q49784 (SYC2_MYCLE)

Names and origin

Protein names

Recommended name:
    Putative cysteinyl-tRNA synthetase 2
    EC=6.1.1.16
Alternative name(s):
    Cysteine--tRNA ligase 2
      Short name=CysRS 2

Gene names

Name:	cysS2
Ordered Locus Names:	ML1302
ORF Names:	B2126_C2_193

Organism

Mycobacterium leprae [Complete proteome] [HAMAP]

Taxonomic identifier

1769 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	412 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00041
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.
Sequence caution	The sequence AAA17194.1 differs from that shown. Reason: Frameshift at several positions.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP cysteine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 412	412	Putative cysteinyl-tRNA synthetase 2 HAMAP MF_00041		PRO_0000159438
Regions
	Motif	46 – 56	11	"HIGH" region HAMAP MF_00041
	Motif	289 – 293	5	"KMSKS" region HAMAP MF_00041
Sites
	Binding site	292	1	ATP By similarity
Experimental info
	Sequence conflict	274	1	A → AGTTCTLG Ref.1
	Sequence conflict	305	1	A → P Ref.1
	Sequence conflict	309	1	P → R Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q49784-1 [UniParc]. Last modified April 27, 2001. Version 3. Checksum: 48165EF99409A25B	FASTA	412	45,293
	10 20 30 40 50 60 MVFRTGSGVA GRGPELRLYD TSDRQVRPVS AAVAPTCKAT MYVCGITPYD ATHLGHAATY 70 80 90 100 110 120 LAFDLIHRLW LDLGHEVHYV QNVTDVDDPL FERADRNGVD WRDLAEREVA LFRDDMASLR 130 140 150 160 170 180 ILPPHDYVAA TETIVEIVEL VDKMLVSGAA YVIDDEYPDI YFRADATLQF GYESGYDRDT 190 200 210 220 230 240 MLRLYEQSGG DPRRPGKNGE LDALLWRAAR PGEPSWSSPF GPGRPGWHVE CAAIALSRIG 250 260 270 280 290 300 IGLDIQGGGS DLIFPHHEFT AAHAECVRGE RRFARHYVHA GMIGWDEHKM SKSRGNLVLV 310 320 330 340 350 360 STLRAQGAPP SAIRLGLLAG HYRADRFWSS QLLDDAIARL HRWRTAASMP AGPDVADVIA 370 380 390 400 410 RVRGYLADDL DTPKAIAALD GWVTDALEYG GHDAAAPKLL ATAIDALLGV DL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Smith D.R., Robison K. Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Massive gene decay in the leprosy bacillus." Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S. , Feltwell T., Fraser A., Hamlin N., Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S., Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R., Barrell B.G. Nature 409:1007-1011(2001) [PubMed: 11234002] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TN.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U00017 Genomic DNA. Translation: AAA17194.1. Frameshift. AL583921 Genomic DNA. Translation: CAC31683.1. Different initiation.
PIR	H87071.
RefSeq	NP_301936.1.
3D structure databases
HSSP	HSSP built from PDB template 1LI5 based on UniProtKB P21888.
ModBase	Search...
Genome annotation databases
GeneID	910422.
GenomeReviews	Gene locus ML1302 in contig AL450380_GR.
KEGG	mle:ML1302.
NMPDR	fig|272631.1.peg.808.
Organism-specific databases
Leproma	ML1302.
CMR	Search...
Phylogenomic databases
HOGENOM	Q49784.
Enzyme and pathway databases
BioCyc	MLEP272631:ML1302-MON.
BRENDA	6.1.1.16. 808.
Family and domain databases
HAMAP	MF_00041. Divergent sequence. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR015804. Cys-tRNA-synt_Ia_C. IPR015803. Cys-tRNA-synt_Ia_N. IPR017812. Mycothiol_biosynthesis_MshC. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
TIGRFAMs	TIGR03447. mycothiol_MshC. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC2_MYCLE

Accession

Primary (citable) accession number: Q49784

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	April 27, 2001
Last modified:	June 16, 2009
This is version 65 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)