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Protein

L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase

Gene

mshC

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent condensation of GlcN-Ins and L-cysteine to form L-Cys-GlcN-Ins.By similarity

Catalytic activityi

1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP = 1-O-(2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + AMP + diphosphate.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi44 – 441ZincBy similarity
Binding sitei59 – 591Cysteinyl adenylateBy similarity
Binding sitei227 – 2271Cysteinyl adenylateBy similarity
Metal bindingi231 – 2311ZincBy similarity
Metal bindingi256 – 2561ZincBy similarity
Binding sitei283 – 2831Cysteinyl adenylate; via amide nitrogen and carbonyl oxygenBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase (EC:6.3.1.13)
Short name:
L-Cys:GlcN-Ins ligase
Alternative name(s):
Mycothiol ligase
Short name:
MSH ligase
Gene namesi
Name:mshC
Synonyms:cysS2
Ordered Locus Names:ML1302
ORF Names:B2126_C2_193
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000806 Componenti: Chromosome

Organism-specific databases

LepromaiML1302.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 412412L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligasePRO_0000159438Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi272631.ML1302.

Structurei

3D structure databases

ProteinModelPortaliQ49784.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni44 – 474Cysteinyl adenylate bindingBy similarity
Regioni82 – 843Cysteinyl adenylate bindingBy similarity
Regioni249 – 2513Cysteinyl adenylate bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi46 – 5611"HIGH" regionAdd
BLAST
Motifi187 – 1926"ERGGDP" region
Motifi289 – 2935"KMSKS" region

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0215. LUCA.
HOGENOMiHOG000245251.
OrthoDBiPOG091H004K.

Family and domain databases

CDDicd00672. CysRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01697. MshC. 1 hit.
InterProiIPR024909. Cys-tRNA/MSH_ligase.
IPR017812. Mycothiol_ligase_MshC.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
TIGRFAMsiTIGR03447. mycothiol_MshC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q49784-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVFRTGSGVA GRGPELRLYD TSDRQVRPVS AAVAPTCKAT MYVCGITPYD
60 70 80 90 100
ATHLGHAATY LAFDLIHRLW LDLGHEVHYV QNVTDVDDPL FERADRNGVD
110 120 130 140 150
WRDLAEREVA LFRDDMASLR ILPPHDYVAA TETIVEIVEL VDKMLVSGAA
160 170 180 190 200
YVIDDEYPDI YFRADATLQF GYESGYDRDT MLRLYEQSGG DPRRPGKNGE
210 220 230 240 250
LDALLWRAAR PGEPSWSSPF GPGRPGWHVE CAAIALSRIG IGLDIQGGGS
260 270 280 290 300
DLIFPHHEFT AAHAECVRGE RRFARHYVHA GMIGWDEHKM SKSRGNLVLV
310 320 330 340 350
STLRAQGAPP SAIRLGLLAG HYRADRFWSS QLLDDAIARL HRWRTAASMP
360 370 380 390 400
AGPDVADVIA RVRGYLADDL DTPKAIAALD GWVTDALEYG GHDAAAPKLL
410
ATAIDALLGV DL
Length:412
Mass (Da):45,293
Last modified:April 27, 2001 - v3
Checksum:i48165EF99409A25B
GO

Sequence cautioni

The sequence AAA17194 differs from that shown. Reason: Frameshift at several positions. Curated
The sequence CAC31683 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00017 Genomic DNA. Translation: AAA17194.1. Frameshift.
AL583921 Genomic DNA. Translation: CAC31683.1. Different initiation.
PIRiH87071.
RefSeqiWP_010908257.1. NC_002677.1.

Genome annotation databases

EnsemblBacteriaiCAC31683; CAC31683; CAC31683.
PATRICi18054934. VBIMycLep78757_2404.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00017 Genomic DNA. Translation: AAA17194.1. Frameshift.
AL583921 Genomic DNA. Translation: CAC31683.1. Different initiation.
PIRiH87071.
RefSeqiWP_010908257.1. NC_002677.1.

3D structure databases

ProteinModelPortaliQ49784.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272631.ML1302.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC31683; CAC31683; CAC31683.
PATRICi18054934. VBIMycLep78757_2404.

Organism-specific databases

LepromaiML1302.

Phylogenomic databases

eggNOGiCOG0215. LUCA.
HOGENOMiHOG000245251.
OrthoDBiPOG091H004K.

Family and domain databases

CDDicd00672. CysRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01697. MshC. 1 hit.
InterProiIPR024909. Cys-tRNA/MSH_ligase.
IPR017812. Mycothiol_ligase_MshC.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
TIGRFAMsiTIGR03447. mycothiol_MshC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMSHC_MYCLE
AccessioniPrimary (citable) accession number: Q49784
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: April 27, 2001
Last modified: September 7, 2016
This is version 109 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.