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Protein

Proteasome subunit beta

Gene

prcB

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation.UniRule annotation

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.UniRule annotation

Enzyme regulationi

The formation of the proteasomal ATPase ARC-20S proteasome complex, likely via the docking of the C-termini of ARC into the intersubunit pockets in the alpha-rings, may trigger opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei57 – 571NucleophileUniRule annotation

GO - Molecular functioni

  1. threonine-type endopeptidase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. modification-dependent protein catabolic process Source: UniProtKB-HAMAP
  2. proteasomal protein catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Enzyme and pathway databases

UniPathwayiUPA00997.

Protein family/group databases

MEROPSiT01.005.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit betaUniRule annotation (EC:3.4.25.1UniRule annotation)
Alternative name(s):
20S proteasome beta subunitUniRule annotation
Proteasome core protein PrcBUniRule annotation
Gene namesi
Name:prcBUniRule annotation
Ordered Locus Names:ML1322
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000000806: Chromosome

Organism-specific databases

LepromaiML1322.

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. proteasome core complex, beta-subunit complex Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 5656Removed in mature form; by autocatalysisUniRule annotationPRO_0000397532Add
BLAST
Chaini57 – 291235Proteasome subunit betaPRO_0000397533Add
BLAST

Keywords - PTMi

Autocatalytic cleavage, Zymogen

Interactioni

Subunit structurei

The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped by the proteasome-associated ATPase, ARC.UniRule annotation

Protein-protein interaction databases

STRINGi272631.ML1322.

Structurei

3D structure databases

ProteinModelPortaliQ49780.
SMRiQ49780. Positions 22-278.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0638.
HOGENOMiHOG000245308.
KOiK03433.
OMAiFQVELEH.
OrthoDBiEOG6XM79W.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_02113_B. Proteasome_B_B.
InterProiIPR029055. Ntn_hydrolases_N.
IPR022483. Pept_T1A_Psome_suB_actinobac.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR03690. 20S_bact_beta. 1 hit.
PROSITEiPS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q49780-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRSFPDRLP TNLAFPGISV INQSSFVDLL RRQAPELLPV SLGGGQSGGG
60 70 80 90 100
QQLSHGTTIV VLKYPGGVVI AGDRRSTQGN MIAGRDVRKV YITDDYTATG
110 120 130 140 150
IAGIAAVAVE FARLYAVELE HYEKLEGVPL TFAGKVNRLA IMVRSNLTAA
160 170 180 190 200
MQGLLALPLL AGYDIHAPDP QSAGRIVSFD AAGGWNIEEE GYQSVGSGSI
210 220 230 240 250
FAKSSIKKLY SQVSDADSAL RVAIEALYDA ADDDSATGGP DLVRGIYPTA
260 270 280 290
VTIGAEGAAE VTESRIAELA REIIESRSRA YTLGSFGGSE K
Length:291
Mass (Da):30,639
Last modified:November 1, 1996 - v1
Checksum:iBCEACCA38EF4C6C3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00017 Genomic DNA. Translation: AAA17188.1.
AL035310 Genomic DNA. Translation: CAA22933.1.
AL583921 Genomic DNA. Translation: CAC31703.1.
PIRiS72848.
RefSeqiNP_301950.1. NC_002677.1.
WP_010908271.1. NC_002677.1.

Genome annotation databases

EnsemblBacteriaiCAC31703; CAC31703; CAC31703.
GeneIDi910447.
KEGGimle:ML1322.
PATRICi18055002. VBIMycLep78757_2439.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00017 Genomic DNA. Translation: AAA17188.1.
AL035310 Genomic DNA. Translation: CAA22933.1.
AL583921 Genomic DNA. Translation: CAC31703.1.
PIRiS72848.
RefSeqiNP_301950.1. NC_002677.1.
WP_010908271.1. NC_002677.1.

3D structure databases

ProteinModelPortaliQ49780.
SMRiQ49780. Positions 22-278.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272631.ML1322.

Protein family/group databases

MEROPSiT01.005.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC31703; CAC31703; CAC31703.
GeneIDi910447.
KEGGimle:ML1322.
PATRICi18055002. VBIMycLep78757_2439.

Organism-specific databases

LepromaiML1322.

Phylogenomic databases

eggNOGiCOG0638.
HOGENOMiHOG000245308.
KOiK03433.
OMAiFQVELEH.
OrthoDBiEOG6XM79W.

Enzyme and pathway databases

UniPathwayiUPA00997.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_02113_B. Proteasome_B_B.
InterProiIPR029055. Ntn_hydrolases_N.
IPR022483. Pept_T1A_Psome_suB_actinobac.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR03690. 20S_bact_beta. 1 hit.
PROSITEiPS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TN.

Entry informationi

Entry nameiPSB_MYCLE
AccessioniPrimary (citable) accession number: Q49780
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: November 1, 1996
Last modified: January 7, 2015
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.