Q49729 (IMDH_MYCLE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inosine-5'-monophosphate dehydrogenase
Short name=IMP dehydrogenase
Short name=IMPD
Short name=IMPDH
EC=1.1.1.205

Gene names

Name:	guaB
Ordered Locus Names:	ML0387
ORF Names:	B1620_C3_238

Organism

Mycobacterium leprae [Complete proteome] [HAMAP]

Taxonomic identifier

1769 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	529 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Inosine 5'-phosphate + NAD+ + H2O = xanthosine 5'-phosphate + NADH.
Pathway	Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the IMPDH/GMPR family. Contains 2 CBS domains.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Domain	CBS domain Repeat
Ligand	Metal-binding NAD Potassium
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	IMP dehydrogenase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 529	529	Inosine-5'-monophosphate dehydrogenase		PRO_0000093701
Regions
	Domain	129 – 185	57	CBS 1
	Domain	189 – 246	58	CBS 2
	Nucleotide binding	262 – 285	24	NAD By similarity
Sites
	Active site	341	1	Thioimidate intermediate By similarity
	Metal binding	336	1	Potassium; via carbonyl oxygen By similarity
	Metal binding	338	1	Potassium; via carbonyl oxygen By similarity
	Binding site	374	1	IMP By similarity
	Binding site	421	1	IMP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q49729 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 1565C62EC9529870	FASTA	529	54,814
	10 20 30 40 50 60 MIRGMSNLKE SSDFVASSYV RLGGLMDDPA ATGGDNPHKV AMLGLTFDDV LLLPAASDVV 70 80 90 100 110 120 PATADISSQL TKKIRLKVPL VSSAMDTVTE ARMAIAMARA GGMGVLHRNL PVGEQAGQVE 130 140 150 160 170 180 TVKRSEAGMV TDPVTCRPDN TLAQVGALCA RFRISGLPVV DDSGALAGII TNRDMRFEVD 190 200 210 220 230 240 QSKQVAEVMT KTPLITAAEG VSADAALGLL RRNKIEKLPV VDGHGRLTGL ITVKDFVKTE 250 260 270 280 290 300 QHPLATKDND GRLLVGAAVG VGGDAWVRAM MLVDAGVDVL IVDTAHAHNR LVLDMVGKLK 310 320 330 340 350 360 VEIGDRVQVI GGNVATRSAA AALVEAGADA VKVGVGPGST CTTRVVAGVG APQITAILEA 370 380 390 400 410 420 VAACGPAGVP VIADGGLQYS GDIAKALAAG ASTTMLGSLL AGTAEAPGEL IFVNGKQFKS 430 440 450 460 470 480 YRGMGSLGAM QGRGGDKSYS KDRYFADDAL SEDKLVPEGI EGRVPFRGPL SSVIHQLVGG 490 500 510 520 LRAAMGYTGS PTIEVLQQAQ FVRITPAGLK ESHPHDVAMT VEAPNYYPR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Smith D.R., Robison K. Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Massive gene decay in the leprosy bacillus." Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S. , Feltwell T., Fraser A., Hamlin N., Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S., Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R., Barrell B.G. Nature 409:1007-1011(2001) [PubMed: 11234002] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TN.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00015 Genomic DNA. Translation: AAC43232.1. AL583918 Genomic DNA. Translation: CAC29895.1.
PIR	S72823.
RefSeq	NP_301377.1. NC_002677.1.
3D structure databases
ProteinModelPortal	Q49729.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000028623; EBMYCP00000028229; EBMYCG00000028618.
GeneID	909092.
GenomeReviews	Gene locus ML0387 in contig AL450380_GR.
KEGG	mle:ML0387.
NMPDR	fig|272631.1.peg.249.
PATRIC	18051408. VBIMycLep78757_0653.
Organism-specific databases
Leproma	ML0387.
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000017422.
HOGENOM	HBG298985.
OMA	GIGIVHK.
ProtClustDB	PRK05567.
Enzyme and pathway databases
BioCyc	MLEP272631:ML0387-MONOMER.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR000644. Cysta_beta_synth_core. IPR005990. IMP_DH. IPR018529. IMP_DH-rel. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DH_GMPRt. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K00088.
PANTHER	PTHR11911:SF6. IMP_DH_GMPRtase. 1 hit.
Pfam	PF00571. CBS. 2 hits. PF00478. IMPDH. 1 hit. [Graphical view]
PIRSF	PIRSF000130. IMPDH. 1 hit.
SMART	SM00116. CBS. 2 hits. [Graphical view]
TIGRFAMs	TIGR01302. IMP_dehydrog. 1 hit.
PROSITE	PS51371. CBS. 2 hits. PS00487. IMP_DH_GMP_RED. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IMDH_MYCLE

Accession

Primary (citable) accession number: Q49729

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program