Q496M5 (PLK5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inactive serine/threonine-protein kinase PLK5

Alternative name(s):
Polo-like kinase 5
Short name=PLK-5

Gene names

Name:	PLK5
Synonyms:	PLK5P
ORF Names:	FG060302

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inactive serine/threonine-protein kinase that plays a role in cell cycle progression and neuronal differentiation. Ref.5
Subcellular location	Nucleus › nucleolus By similarity. Cytoplasm Ref.5.
Tissue specificity	Expressed in the brain, neurons and glial cells. Also expressed in highly differentiated cells, such as the serous acini in the parotid gland, distal and proximal tubules of the kidney, tubules of the seminal gland, Kupffer cells and some hepatocytes in the liver, and some cells in the germinal center of lymph nodes (at protein level). Ref.5
Induction	Down-regulated in primary brain tumors. Ref.5
Domain	The truncated protein kinase domain is predicted to be catalytically inactive; has lost the main activatory autophosphorylation site and the conserved key residues involved in phospho-substrate. The C-terminus region (containing the POLO box domain) is sufficient for inducing cell cycle arrest.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily. Contains 1 POLO box domain. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Differentiation Mitosis
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G2 DNA damage checkpoint Inferred from mutant phenotype PubMed 20100802. Source: UniProtKB cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular response to growth factor stimulus Inferred from direct assay Ref.5. Source: UniProtKB defense response to tumor cell Inferred from direct assay Ref.5. Source: UniProtKB mitosis Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of neuron projection development Inferred from direct assay Ref.5. Source: UniProtKB regulation of G1/S transition of mitotic cell cycle Inferred from mutant phenotype Ref.5. Source: UniProtKB transformed cell apoptotic process Inferred from mutant phenotype Ref.5. Source: UniProtKB
Cellular_component	cytoplasm Inferred from direct assay Ref.5. Source: UniProtKB nucleolus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: InterPro protein kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q496M5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q496M5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     239-275: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	Inactive serine/threonine-protein kinase PLK5		PRO_0000280397
Regions
	Domain	1 – 65	65	Protein kinase; truncated
	Domain	259 – 334	76	POLO box
	Compositional bias	265 – 268	4	Poly-Leu
Natural variations
	Alternative sequence	239 – 275	37	Missing in isoform 2.		VSP_023654
Experimental info
	Sequence conflict	314	1	R → Q in BAG51426. Ref.1
	Sequence conflict	314	1	R → Q in BC100791. Ref.4
	Sequence conflict	320	1	Missing in ABD83663. Ref.2
	Sequence conflict	323	1	G → R in ABD83663. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 5, 2010. Version 4. Checksum: 782A33DF18A7E445	FASTA	336	36,329
	10 20 30 40 50 60 MYTVLTGTPP FMASPLSEMY QNIREGHYPE PAHLSANARR LIVHLLAPNP AERPSLDHLL 70 80 90 100 110 120 QDDFFTQGFT PDRLPAHSCH SPPIFAIPPP LGRIFRKVGQ RLLTQCRPPC PFTPKEASGP 130 140 150 160 170 180 GEGGPDPDSM EWDGESSLSA KEVPCLEGPI HLVAQGTLQS DLAGPEGSRR PEVEAALRHL 190 200 210 220 230 240 QLCLDVGPPA TQDPLGEQQP ILWAPKWVDY SSKYGFGYQL LDGGRTGRHP HGPATPRREG 250 260 270 280 290 300 TLPTPVPPAG PGLCLLRFLA SEHALLLLFS NGMVQVSFSG VPAQLVLSGE GEGLQLTLWE 310 320 330 QGSPGTSYSL DVPRSHGCAP TTGQHLHHAL RMLQSI
	Isoform 2 [UniParc]. Checksum: 96FCA8A80BEDA446	FASTA	299	32,487
	10 20 30 40 50 60 MYTVLTGTPP FMASPLSEMY QNIREGHYPE PAHLSANARR LIVHLLAPNP AERPSLDHLL 70 80 90 100 110 120 QDDFFTQGFT PDRLPAHSCH SPPIFAIPPP LGRIFRKVGQ RLLTQCRPPC PFTPKEASGP 130 140 150 160 170 180 GEGGPDPDSM EWDGESSLSA KEVPCLEGPI HLVAQGTLQS DLAGPEGSRR PEVEAALRHL 190 200 210 220 230 240 QLCLDVGPPA TQDPLGEQQP ILWAPKWVDY SSKYGFGYQL LDGGRTGRHP HGPATPRRVS 250 260 270 280 290 FSGVPAQLVL SGEGEGLQLT LWEQGSPGTS YSLDVPRSHG CAPTTGQHLH HALRMLQSI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Cerebellum.
[2]	Li H., Nong W., Zhou G., Ke R., Shen C., Zhong G., Liang M., Tang Z., Huang B., Lin L., Yang S. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[3]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]	"Plk5, a polo box domain-only protein with specific roles in neuron differentiation and glioblastoma suppression." de Carcer G., Escobar B., Higuero A.M., Garcia L., Anson A., Perez G., Mollejo M., Manning G., Melendez B., Abad-Rodriguez J., Malumbres M. Mol. Cell. Biol. 31:1225-1239(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INDUCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK054808 mRNA. Translation: BAG51426.1. DQ424898 mRNA. Translation: ABD83663.1. AC027307 Genomic DNA. No translation available. BC100791 mRNA. No translation available.
IPI	IPI00783437. IPI00829623.
RefSeq	NP_001230008.1. NM_001243079.1.
UniGene	Hs.195352.
3D structure databases
ProteinModelPortal	Q496M5.
SMR	Q496M5. Positions 1-89, 194-336.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000334428.
PTM databases
PhosphoSite	Q496M5.
Polymorphism databases
DMDM	308153664.
Proteomic databases
PaxDb	Q496M5.
PRIDE	Q496M5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000334770; ENSP00000466248; ENSG00000185988. ENST00000454744; ENSP00000468376; ENSG00000185988.
GeneID	126520.
KEGG	hsa:126520.
UCSC	uc002ltf.3. human. uc010dsm.1. human.
Organism-specific databases
CTD	126520.
GeneCards	GC19P001525.
HGNC	HGNC:27001. PLK5.
HPA	HPA035024.
neXtProt	NX_Q496M5.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG290091.
HOVERGEN	HBG001843.
InParanoid	Q496M5.
Gene expression databases
Bgee	Q496M5.
Genevestigator	Q496M5.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS50078. POLO_BOX. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	126520.
NextBio	81836.

Entry information

Entry name

PLK5_HUMAN

Accession

Primary (citable) accession number: Q496M5
Secondary accession number(s): B3KNR4, Q1ZYM0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 2007
Last sequence update:	October 5, 2010
Last modified:	May 1, 2013
This is version 67 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.