Reviewed, UniProtKB/Swiss-Prot Q49689 (Y593_MYCLE)

Names and origin

Protein names

Recommended name:
    UPF0051 protein ML0593
Cleaved into the following chain:
    1- Recommended name:
            Mle pps1 intein

Gene names

Ordered Locus Names:	ML0593
ORF Names:	MLCL536.28c, B1496_C2_189

Organism

Mycobacterium leprae [Complete proteome] [HAMAP]

Taxonomic identifier

1769 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	869 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential.
Sequence similarities	Belongs to the UPF0051 (ycf24) family. Contains 1 DOD-type homing endonuclease domain.

Ontologies

Keywords
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro iron-sulfur cluster assembly Inferred from electronic annotation. Source: InterPro
Molecular function	endonuclease activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 201	201	UPF0051 protein ML0593, 1st part Potential		PRO_0000036187
	Chain	202 – 587	386	Mle pps1 intein Potential		PRO_0000036188
	Chain	588 – 869	282	UPF0051 protein ML0593, 2nd part Potential		PRO_0000036189
Regions
	Domain	344 – 477	134	DOD-type homing endonuclease
Experimental info
	Sequence conflict	482	1	A → R in AAA17127. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q49689-1 [UniParc]. Last modified April 27, 2001. Version 2. Checksum: DB04CF70CB50765A	FASTA	869	95,573
	10 20 30 40 50 60 MTRTSETTKS PAPELLTQQQ AIDSLGKYGY GWADSDVAGA SARRGLSEDV VRDISAKKDE 70 80 90 100 110 120 PEWMLQARLK ALRVFERKPM PRWGSNLDGI DFDNIKYFVR STEKQAASWD ELPEDIRNTY 130 140 150 160 170 180 DRLGIPDAEK QRLVAGVAAQ YESEVVYHQI RADLKDQGVV FLDTETGLRE YPDIFKQYLG 190 200 210 220 230 240 TVIPAGDNKF SALNTAVWSG GCLTADARIN VKGKGLVSIA DVQPGDEVFG VNIGCELERG 250 260 270 280 290 300 KVLAKVASGT KPVYEMHVAG RALEATGNHQ FLVARRVEEG KRTRWTAVWA PLEEIESGEP 310 320 330 340 350 360 IAVARVLPDD SGTIFFSESE LDIKNRTRQC LYFPCQNSVD LLWLLGLWLG DGHTAAPHKH 370 380 390 400 410 420 MRQVAFSVPA GDPVHHTAIR VVSEQFGANV TVVNCGFIVS SKAFETWLAE LGFSGDEKTK 430 440 450 460 470 480 RLPAWIYSLP HEHQLALIGG LVDADGWTES SGATMSIAFA SRELLEDVRQ LAIGCGLYPD 490 500 510 520 530 540 GALVERTRSA TCRDGRIVTS TSWRLRIQGS LDRVGTRTPG KRGKPVSNKG RRQRYVAAAG 550 560 570 580 590 600 LNFSSLSTDT VGFARLKSKT LVGEKPTYDI QVVGLENFVA NGIVAHNSFI YVPPGVHVDI 610 620 630 640 650 660 PLQAYFRINT ENMGQFERTL IIADTGSYVH YVEGCTAPIY KSDSLHSAVV EIIVKPHARV 670 680 690 700 710 720 RYTTIQNWSN NVYNLVTKRA RVETGATMEW IDGNIGSKVT MKYPAVWMTG EHAKGEVLSV 730 740 750 760 770 780 AFAGEGQHQD TGAKMLHLAS NTSSNIVSKS VARGGGRTSY RGLVQVNKGA HGSRSSVKCD 790 800 810 820 830 840 ALLVDTISRS DTYPYVDIRE DDVTMGHEAT VSKVSENQLF YLMSRGLAED EAMAMVVRGF 850 860 VEPIAKELPM EYALELNRLI ELQMEGAVG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Smith D.R., Robison K. Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Massive gene decay in the leprosy bacillus." Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S. , Feltwell T., Fraser A., Hamlin N., Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S., Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R., Barrell B.G. Nature 409:1007-1011(2001) [PubMed: 11234002] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TN.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U00013 Genomic DNA. Translation: AAA17127.1. Z99125 Genomic DNA. Translation: CAB16171.1. Z99125 Genomic DNA. Translation: CAB16172.1. AL583919 Genomic DNA. Translation: CAC30101.1.
PIR	A86983. S72760.
RefSeq	NP_301502.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	909370.
GenomeReviews	Gene locus ML0593 in contig AL450380_GR.
KEGG	mle:ML0593.
NMPDR	fig|272631.1.peg.374.
Organism-specific databases
Leproma	ML0593.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG129052.
Enzyme and pathway databases
BioCyc	MLEP272631:ML0593-MON.
Family and domain databases
InterPro	IPR003586. Hedgehog_hint_C. IPR003587. Hedgehog_hint_N. IPR006142. INTEIN. IPR004042. Intein_endonuc. IPR006141. Intein_splicing_site. IPR010231. SUF_FeS_clus_asmbl_SufB. IPR000825. SUF_FeS_clus_asmbl_SufBD. [Graphical view]
Pfam	PF01458. UPF0051. 1 hit. [Graphical view]
PRINTS	PR00379. INTEIN.
SMART	SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. [Graphical view]
PROSITE	PS50818. INTEIN_C_TER. 1 hit. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Y593_MYCLE

Accession

Primary (citable) accession number: Q49689
Secondary accession number(s): O33141

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	April 27, 2001
Last modified:	December 15, 2009
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)