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Protein

Tungsten-containing formylmethanofuran dehydrogenase 2 subunit G

Gene

fwdG

Organism
Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the reversible oxidation of CO2 and methanofuran (MFR) to N-formylmethanofuran (CHO-MFR). This enzyme is oxygen-labile. May function as an electron transfer protein (By similarity).By similarity

Catalytic activityi

Formylmethanofuran + H2O + acceptor = CO2 + methanofuran + reduced acceptor.

Cofactori

[4Fe-4S] clusterCuratedNote: Binds 2 [4Fe-4S] clusters.Curated

Enzyme regulationi

Not inactivated by cyanide.By similarity

Pathwayi: methanogenesis from CO(2)

This protein is involved in step 1 of the subpathway that synthesizes 5,10-methenyl-5,6,7,8-tetrahydromethanopterin from CO(2).
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Tungsten-containing formylmethanofuran dehydrogenase 2 subunit G (fwdG)
  2. Formylmethanofuran--tetrahydromethanopterin formyltransferase (ftr)
  3. Methenyltetrahydromethanopterin cyclohydrolase (mch)
This subpathway is part of the pathway methanogenesis from CO(2), which is itself part of One-carbon metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5,10-methenyl-5,6,7,8-tetrahydromethanopterin from CO(2), the pathway methanogenesis from CO(2) and in One-carbon metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi11Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi14Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi17Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi21Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi60Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi63Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi66Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi70Iron-sulfur 1 (4Fe-4S)By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Methanogenesis, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00640; UER00692.

Names & Taxonomyi

Protein namesi
Recommended name:
Tungsten-containing formylmethanofuran dehydrogenase 2 subunit G (EC:1.2.99.5)
Alternative name(s):
Tungsten-containing formylmethanofuran dehydrogenase II subunit G
Gene namesi
Name:fwdG
Synonyms:fudG
Ordered Locus Names:MK1525
OrganismiMethanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Taxonomic identifieri190192 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanopyriMethanopyralesMethanopyraceaeMethanopyrus
Proteomesi
  • UP000001826 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001592541 – 79Tungsten-containing formylmethanofuran dehydrogenase 2 subunit GAdd BLAST79

Expressioni

Inductioni

By growth on tungsten or molybdenum under anaerobic conditions.

Interactioni

Protein-protein interaction databases

STRINGi190192.MK1525.

Structurei

3D structure databases

ProteinModelPortaliQ49611.
SMRiQ49611.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 314Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd BLAST30
Domaini51 – 794Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd BLAST29

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiarCOG00292. Archaea.
ENOG4111IC0. LUCA.
HOGENOMiHOG000044611.
KOiK11260.
OMAiETWGGKG.
OrthoDBiPOG093Z03NL.

Family and domain databases

InterProiView protein in InterPro
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
PfamiView protein in Pfam
PF00037. Fer4. 2 hits.
PROSITEiView protein in PROSITE
PS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.

Sequencei

Sequence statusi: Complete.

Q49611-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKIVIHEER CHGCGNCVIA CPVNACNSPN VWGGKGPEDG EDVVIKVVNG
60 70
TVSVINEDLC EACMTCELAC PVDAIEIKT
Length:79
Mass (Da):8,345
Last modified:November 1, 1996 - v1
Checksum:i0EFD1B298A8BBA67
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98917 Genomic DNA. Translation: CAA67417.1.
AE009439 Genomic DNA. Translation: AAM02738.1.

Genome annotation databases

EnsemblBacteriaiAAM02738; AAM02738; MK1525.
KEGGimka:MK1525.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98917 Genomic DNA. Translation: CAA67417.1.
AE009439 Genomic DNA. Translation: AAM02738.1.

3D structure databases

ProteinModelPortaliQ49611.
SMRiQ49611.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi190192.MK1525.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM02738; AAM02738; MK1525.
KEGGimka:MK1525.

Phylogenomic databases

eggNOGiarCOG00292. Archaea.
ENOG4111IC0. LUCA.
HOGENOMiHOG000044611.
KOiK11260.
OMAiETWGGKG.
OrthoDBiPOG093Z03NL.

Enzyme and pathway databases

UniPathwayiUPA00640; UER00692.

Family and domain databases

InterProiView protein in InterPro
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
PfamiView protein in Pfam
PF00037. Fer4. 2 hits.
PROSITEiView protein in PROSITE
PS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiFWDG_METKA
AccessioniPrimary (citable) accession number: Q49611
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: November 1, 1996
Last modified: March 15, 2017
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.