Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Tungsten-containing formylmethanofuran dehydrogenase 2 subunit G

Gene

fwdG

Organism
Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the reversible oxidation of CO2 and methanofuran (MFR) to N-formylmethanofuran (CHO-MFR). This enzyme is oxygen-labile. May function as an electron transfer protein (By similarity).By similarity

Catalytic activityi

Formylmethanofuran + H2O + acceptor = CO2 + methanofuran + reduced acceptor.

Cofactori

[4Fe-4S] clusterCuratedNote: Binds 2 [4Fe-4S] clusters.Curated

Enzyme regulationi

Not inactivated by cyanide.By similarity

Pathwayi: methanogenesis from CO(2)

This protein is involved in step 1 of the subpathway that synthesizes 5,10-methenyl-5,6,7,8-tetrahydromethanopterin from CO(2).
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Tungsten-containing formylmethanofuran dehydrogenase 2 subunit G (fwdG)
  2. Formylmethanofuran--tetrahydromethanopterin formyltransferase (ftr)
  3. Methenyltetrahydromethanopterin cyclohydrolase (mch)
This subpathway is part of the pathway methanogenesis from CO(2), which is itself part of One-carbon metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5,10-methenyl-5,6,7,8-tetrahydromethanopterin from CO(2), the pathway methanogenesis from CO(2) and in One-carbon metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi11Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi14Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi17Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi21Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi60Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi63Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi66Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi70Iron-sulfur 1 (4Fe-4S)By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Methanogenesis, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00640; UER00692.

Names & Taxonomyi

Protein namesi
Recommended name:
Tungsten-containing formylmethanofuran dehydrogenase 2 subunit G (EC:1.2.99.5)
Alternative name(s):
Tungsten-containing formylmethanofuran dehydrogenase II subunit G
Gene namesi
Name:fwdG
Synonyms:fudG
Ordered Locus Names:MK1525
OrganismiMethanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Taxonomic identifieri190192 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanopyriMethanopyralesMethanopyraceaeMethanopyrus
Proteomesi
  • UP000001826 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001592541 – 79Tungsten-containing formylmethanofuran dehydrogenase 2 subunit GAdd BLAST79

Expressioni

Inductioni

By growth on tungsten or molybdenum under anaerobic conditions.

Interactioni

Protein-protein interaction databases

STRINGi190192.MK1525.

Structurei

3D structure databases

ProteinModelPortaliQ49611.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 314Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd BLAST30
Domaini51 – 794Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd BLAST29

Sequence similaritiesi

Contains 2 4Fe-4S ferredoxin-type domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiarCOG00292. Archaea.
ENOG4111IC0. LUCA.
HOGENOMiHOG000044611.
KOiK11260.
OMAiETWGGKG.

Family and domain databases

InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
[Graphical view]
PfamiPF00037. Fer4. 2 hits.
[Graphical view]
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q49611-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKIVIHEER CHGCGNCVIA CPVNACNSPN VWGGKGPEDG EDVVIKVVNG
60 70
TVSVINEDLC EACMTCELAC PVDAIEIKT
Length:79
Mass (Da):8,345
Last modified:November 1, 1996 - v1
Checksum:i0EFD1B298A8BBA67
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98917 Genomic DNA. Translation: CAA67417.1.
AE009439 Genomic DNA. Translation: AAM02738.1.

Genome annotation databases

EnsemblBacteriaiAAM02738; AAM02738; MK1525.
KEGGimka:MK1525.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98917 Genomic DNA. Translation: CAA67417.1.
AE009439 Genomic DNA. Translation: AAM02738.1.

3D structure databases

ProteinModelPortaliQ49611.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi190192.MK1525.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM02738; AAM02738; MK1525.
KEGGimka:MK1525.

Phylogenomic databases

eggNOGiarCOG00292. Archaea.
ENOG4111IC0. LUCA.
HOGENOMiHOG000044611.
KOiK11260.
OMAiETWGGKG.

Enzyme and pathway databases

UniPathwayiUPA00640; UER00692.

Family and domain databases

InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
[Graphical view]
PfamiPF00037. Fer4. 2 hits.
[Graphical view]
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFWDG_METKA
AccessioniPrimary (citable) accession number: Q49611
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.