Q49611 (FWDG_METKA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tungsten-containing formylmethanofuran dehydrogenase 2 subunit G
EC=1.2.99.5

Alternative name(s):
Tungsten-containing formylmethanofuran dehydrogenase II subunit G

Gene names

Name:	fwdG
Synonyms:	fudG
Ordered Locus Names:	MK1525

Organism

Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)

Taxonomic identifier

190192 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanopyri › Methanopyrales › Methanopyraceae › Methanopyrus

Protein attributes

Sequence length	79 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the reversible oxidation of CO2 and methanofuran (MFR) to N-formylmethanofuran (CHO-MFR). This enzyme is oxygen-labile. May function as an electron transfer protein By similarity.
Catalytic activity	Formylmethanofuran + H2O + acceptor = CO2 + methanofuran + reduced acceptor.
Cofactor	Binds 2 4Fe-4S clusters Potential.
Enzyme regulation	Not inactivated by cyanide By similarity.
Pathway	One-carbon metabolism; methanogenesis from CO(2); 5,10-methenyl-5,6,7,8-tetrahydromethanopterin from CO(2): step 1/3.
Induction	By growth on tungsten or molybdenum under anaerobic conditions.
Sequence similarities	Contains 2 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Biological process	Electron transport Methanogenesis Transport
Domain	Repeat
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro formylmethanofuran dehydrogenase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 79	79	Tungsten-containing formylmethanofuran dehydrogenase 2 subunit G		PRO_0000159254
Regions
	Domain	2 – 31	30	4Fe-4S ferredoxin-type 1
	Domain	51 – 79	29	4Fe-4S ferredoxin-type 2
Sites
	Metal binding	11	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	14	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	17	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	21	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	60	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	63	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	66	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	70	1	Iron-sulfur 1 (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q49611 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 0EFD1B298A8BBA67	FASTA	79	8,345
	10 20 30 40 50 60 MVKIVIHEER CHGCGNCVIA CPVNACNSPN VWGGKGPEDG EDVVIKVVNG TVSVINEDLC 70 EACMTCELAC PVDAIEIKT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A selenium-dependent and a selenium-independent formylmethanofuran dehydrogenase and their transcriptional regulation in the hyperthermophilic Methanopyrus kandleri." Vorholt J.A., Vaupel M., Thauer R.K. Mol. Microbiol. 23:1033-1042(1997) [PubMed: 9076739] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.
[2]	"The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens." Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N., Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A., Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G., Koonin E.V., Kozyavkin S.A. Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002) [PubMed: 11930014] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X98917 Genomic DNA. Translation: CAA67417.1. AE009439 Genomic DNA. Translation: AAM02738.1.
RefSeq	NP_614808.1. NC_003551.1.
3D structure databases
ProteinModelPortal	Q49611.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1478120.
GenomeReviews	Gene locus MK1525 in contig AE009439_GR.
KEGG	mka:MK1525.
NMPDR	fig|190192.1.peg.1521.
Phylogenomic databases
HOGENOM	HBG734156.
OMA	DLCGGCG.
ProtClustDB	CLSK876480.
Enzyme and pathway databases
BioCyc	MKAN190192:MK1525-MONOMER.
Family and domain databases
InterPro	IPR001450. 4Fe4S-bd_dom. IPR017896. 4Fe4S_Fe-S-bd. IPR017900. 4Fe4S_Fe_S_CS. [Graphical view]
KO	K11260.
Pfam	PF00037. Fer4. 2 hits. [Graphical view]
PROSITE	PS00198. 4FE4S_FER_1. 2 hits. PS51379. 4FE4S_FER_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FWDG_METKA

Accession

Primary (citable) accession number: Q49611

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
Last sequence update:	November 1, 1996
Last modified:	November 16, 2011
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program