Q49608 (GYRA_MYCKA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA gyrase subunit A
EC=5.99.1.3

Cleaved into the following chain:

1. Mka gyrA intein

Gene names

Name:

gyrA

Organism

Mycobacterium kansasii

Taxonomic identifier

1768 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	549 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings. HAMAP MF_01897
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP MF_01897
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Subcellular location	Cytoplasm Potential HAMAP MF_01897.
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation By similarity. HAMAP MF_01897
Sequence similarities	Belongs to the topoisomerase GyrA/ParC subunit family. Contains 1 DOD-type homing endonuclease domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
PTM	Autocatalytic cleavage Protein splicing
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC endonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 65	›65	DNA gyrase subunit A, 1st part By similarity		PRO_0000034804
	Chain	66 – 485	420	Mka gyrA intein By similarity		PRO_0000034805
	Chain	486 – ›549	›64	DNA gyrase subunit A, 2nd part By similarity		PRO_0000034806
Regions
	Domain	191 – 331	141	DOD-type homing endonuclease
Sites
	Active site	65	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	549	1

Sequences

	Sequence		Length	Mass (Da)
	Q49608 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 5560B6B771B8B43F	FASTA	549	61,051
	10 20 30 40 50 60 RPARSHAKSA RSVAETMGNY HPHGDASIYD TLVRMAQPWS LRYPLVDGQG NFGSPGNVPP 70 80 90 100 110 120 AAMRYCVTGD ALVRLPFGQS MRIADVVPGA RPNSDNAVEL KVLDRHGNPV AADRLFHSGD 130 140 150 160 170 180 HQTYMVRTAE GYEVTGTANH PLLCLVDVGG VPTLLWKLIE EIHPDDYVAL QRTPPMELGP 190 200 210 220 230 240 ADWHDTMEAL LLGAFISEGC VSETRAGFAN LDRDYFTMVA RAYDAVVGDK RDVYQQTIAS 250 260 270 280 290 300 GSLQHTLYTQ NVTALKQSRL WQILGMRSAD TYVPEWMWHS PAAVKRVFLQ ALFEGDGSCS 310 320 330 340 350 360 RRPHNTIQIS YNTVSKQLAM DVQQMLLEFG VISRRYLHAA GEYKVVITDR AQAELFPKQI 370 380 390 400 410 420 GFGGAKQTEL SKILAAMPPC AGRDSDHVPG LARFIRRHCD SRWVDKEWLH KHNIDHLSRW 430 440 450 460 470 480 RRDGAEILSH IADPDVRTIA TDLTDGRFYY ARVASVTDTG VQPVYSLRVD TDDHAFLTNG 490 500 510 520 530 540 FVSHNTEARL TPLAMEMLRE IDEETVDFIP NYDGRVQEPT VLPSRFPNLL ANGSGGIAVG MATNIPPHN

References

[1]	"Homing events in the gyrA gene of some mycobacteria." Fsihi H., Vincent V., Cole S.T. Proc. Natl. Acad. Sci. U.S.A. 93:3410-3415(1996) [PubMed: 8622949] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: KANS0 / 930908.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z68207 Genomic DNA. Translation: CAA92431.1.
3D structure databases
ProteinModelPortal	Q49608.
SMR	Q49608. Positions 4-65, 67-173, 429-549.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_01897. GyrA. [Tree]
InterPro	IPR003586. Hedgehog_hint_C. IPR003587. Hedgehog_hint_N. IPR006142. INTEIN. IPR004042. Intein_endonuc. IPR006141. Intein_splice_site. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013760. Topo_IIA_cen. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 2 hits.
Pfam	PF00521. DNA_topoisoIV. 2 hits. [Graphical view]
PRINTS	PR00379. INTEIN.
SMART	SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. SM00434. TOP4c. 1 hit. [Graphical view]
SUPFAM	SSF56719. Topo_IIA_cen. 2 hits.
TIGRFAMs	TIGR01443. Intein_Cterm. 1 hit. TIGR01445. Intein_Nterm. 1 hit.
PROSITE	PS50818. INTEIN_C_TER. 1 hit. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GYRA_MYCKA

Accession

Primary (citable) accession number: Q49608

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	September 21, 2011
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program