Reviewed, UniProtKB/Swiss-Prot Q49606 (MTRE_METKA)

Names and origin

Protein names

Recommended name:
    Tetrahydromethanopterin S-methyltransferase subunit E
    EC=2.1.1.86
Alternative name(s):
    N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit E

Gene names

Name:	mtrE
Ordered Locus Names:	MK0656

Organism

Methanopyrus kandleri [Complete proteome] [HAMAP]

Taxonomic identifier

2320 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanopyri › Methanopyrales › Methanopyraceae › Methanopyrus

Protein attributes

Sequence length	298 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Part of a complex that catalyzes the formation of methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-tetrahydromethanopterin. This is an energy-conserving, sodium-ion translocating step By similarity.
Catalytic activity	5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate = 5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate. HAMAP MF_01098
Pathway	One-carbon metabolism; methanogenesis from carbone dioxide; methyl-CoM from 5,10-methylene-H(4)MPT: step 2/2. HAMAP MF_01098
Subunit structure	The complex is composed of 8 subunits; mtrA, mtrB, mtrC, mtrD, mtrE, mtrF, mtrG and mtrH By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the mtrE family.

Ontologies

Keywords
Biological process	Methanogenesis One-carbon metabolism
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW sodium ion transport Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell vesicle membrane Inferred from electronic annotation. Source: InterPro
Molecular function	tetrahydromethanopterin S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 298	298	Tetrahydromethanopterin S-methyltransferase subunit E HAMAP MF_01098		PRO_0000147540
Regions
	Transmembrane	3 – 23	21	Potential
	Transmembrane	59 – 79	21	Potential
	Transmembrane	84 – 104	21	Potential
	Transmembrane	132 – 152	21	Potential
	Transmembrane	160 – 180	21	Potential
	Transmembrane	230 – 250	21	Potential
	Transmembrane	251 – 271	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q49606-1 [UniParc]. Last modified July 15, 1999. Version 2. Checksum: B540A568D1168C4C	FASTA	298	31,527
	10 20 30 40 50 60 MVGFTEIGLA AAMGALATIA GAFEDAESDV GSQSNPNSQV QLAPQMMNFH RYFNKAISGE 70 80 90 100 110 120 PVSYMLYGAI AGTVTWVMMT KFGLPFLAAA AVGVGVNALI HMVFATTAHL GRMASAAEFG 130 140 150 160 170 180 HPIYLDVVLS HLGPIAGFGG IATFAIVSLA YIQWALLKHP FPLPLLAALW GVTVGAIGSS 190 200 210 220 230 240 TGDVHYGAER LYQHYPFGGG VPVAAHGNIT RKAETGIRNS MDSVYFCAKF GNPLTGLCFG 250 260 270 280 290 LVVFFSTWAG LFGQWGAVIA MGLVTLGCLI VSNRVEKKAR ESYGTYEDVE MDEICDPV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of the active site histidine in the corrinoid protein MtrA of the energy-conserving methyltransferase complex from Methanobacterium thermoautotrophicum." Harms U., Thauer R.K. Eur. J. Biochem. 250:783-788(1997) [PubMed: 9461302] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens." Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N., Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A., Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G., Koonin E.V., Kozyavkin S.A. Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002) [PubMed: 11930014] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.
[3]	"Phylogeny of Methanopyrus kandleri based on methyl coenzyme M reductase operons." Noelling J., Elfner A., Palmer J.R., Steigerwald V.J., Pihl T.D., Lake J.A., Reeve J.N. Int. J. Syst. Bacteriol. 46:1170-1173(1996) [PubMed: 8863453] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-89.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y14428 Genomic DNA. Translation: CAA74774.1. AE010359 Genomic DNA. Translation: AAM01871.1. U57340 Genomic DNA. Translation: AAB02004.1.
RefSeq	NP_613941.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1476757.
GenomeReviews	Gene locus MK0656 in contig AE009439_GR.
KEGG	mka:MK0656.
NMPDR	fig|190192.1.peg.654.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q49606.
OMA	Q49606. SNPNSQV.
Enzyme and pathway databases
BioCyc	MKAN190192:MK0656-MON.
BRENDA	2.1.1.86. 7577.
Family and domain databases
HAMAP	MF_01098. [Tree]
InterPro	IPR005780. MeTrfase_E. [Graphical view]
Pfam	PF04206. MtrE. 1 hit. [Graphical view]
PIRSF	PIRSF016509. MtrE. 1 hit.
TIGRFAMs	TIGR01113. mtrE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MTRE_METKA

Accession

Primary (citable) accession number: Q49606

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 15, 1999
Last modified:	June 16, 2009
This is version 56 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)