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Protein

Methyl-coenzyme M reductase I subunit alpha

Gene

mcrA

Organism
Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Reduction of methyl-coenzyme M (2-(methylthio) ethanesulfonic acid) with 7-mercaptoheptanoylthreonine phosphate to methane and a heterodisulfide.

Catalytic activityi

Methyl-CoM + CoB = CoM-S-S-CoB + methane.

Cofactori

coenzyme F430Note: Binds 1 coenzyme F430 noncovalently per subunit. Coenzyme F430 is a yellow nickel porphinoid.

Pathwayi: methyl-coenzyme M reduction

This protein is involved in step 1 of the subpathway that synthesizes methane from methyl-coenzyme M.
Proteins known to be involved in this subpathway in this organism are:
  1. Methyl-coenzyme M reductase I subunit alpha (mcrA), Methyl-coenzyme M reductase subunit beta (mcrB), Methyl-coenzyme M reductase subunit gamma (mcrG)
This subpathway is part of the pathway methyl-coenzyme M reduction, which is itself part of One-carbon metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes methane from methyl-coenzyme M, the pathway methyl-coenzyme M reduction and in One-carbon metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi150Nickel1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processMethanogenesis
LigandMetal-binding, Nickel

Enzyme and pathway databases

BRENDAi2.8.4.1 3274
UniPathwayiUPA00646; UER00699

Names & Taxonomyi

Protein namesi
Recommended name:
Methyl-coenzyme M reductase I subunit alpha (EC:2.8.4.1)
Short name:
MCR I alpha
Alternative name(s):
Coenzyme-B sulfoethylthiotransferase alpha
Gene namesi
Name:mcrA
Ordered Locus Names:MK0655
OrganismiMethanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Taxonomic identifieri190192 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanopyriMethanopyralesMethanopyraceaeMethanopyrus
Proteomesi
  • UP000001826 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001474541 – 553Methyl-coenzyme M reductase I subunit alphaAdd BLAST553

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei260Pros-methylhistidineBy similarity1
Modified residuei2745-methylarginineBy similarity1

Keywords - PTMi

Methylation

Proteomic databases

PRIDEiQ49605

Interactioni

Subunit structurei

Hexamer of two alpha, two beta, and two gamma chains.

Protein-protein interaction databases

STRINGi190192.MK0655

Structurei

Secondary structure

1553
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi10 – 16Combined sources7
Helixi33 – 36Combined sources4
Helixi38 – 54Combined sources17
Beta strandi74 – 77Combined sources4
Beta strandi83 – 85Combined sources3
Helixi86 – 88Combined sources3
Helixi91 – 93Combined sources3
Helixi95 – 105Combined sources11
Beta strandi107 – 111Combined sources5
Helixi113 – 121Combined sources9
Helixi129 – 142Combined sources14
Turni143 – 145Combined sources3
Turni158 – 160Combined sources3
Beta strandi165 – 171Combined sources7
Helixi173 – 176Combined sources4
Helixi181 – 183Combined sources3
Helixi187 – 190Combined sources4
Helixi193 – 203Combined sources11
Beta strandi207 – 212Combined sources6
Helixi215 – 220Combined sources6
Helixi223 – 242Combined sources20
Helixi251 – 259Combined sources9
Beta strandi262 – 266Combined sources5
Helixi272 – 274Combined sources3
Helixi281 – 283Combined sources3
Helixi286 – 292Combined sources7
Helixi295 – 298Combined sources4
Helixi303 – 319Combined sources17
Helixi320 – 326Combined sources7
Helixi334 – 338Combined sources5
Helixi345 – 360Combined sources16
Turni363 – 365Combined sources3
Helixi370 – 390Combined sources21
Helixi392 – 397Combined sources6
Helixi401 – 420Combined sources20
Helixi423 – 441Combined sources19
Turni447 – 450Combined sources4
Helixi451 – 461Combined sources11
Turni465 – 467Combined sources3
Helixi471 – 473Combined sources3
Helixi479 – 481Combined sources3
Beta strandi485 – 487Combined sources3
Helixi488 – 502Combined sources15
Helixi510 – 515Combined sources6
Beta strandi521 – 523Combined sources3
Helixi528 – 536Combined sources9
Helixi547 – 549Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E6VX-ray2.70A/D1-553[»]
ProteinModelPortaliQ49605
SMRiQ49605
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ49605

Family & Domainsi

Phylogenomic databases

eggNOGiarCOG04857 Archaea
COG4058 LUCA
HOGENOMiHOG000225809
KOiK00399
OMAiGRVCDGG
OrthoDBiPOG093Z00ZI

Family and domain databases

Gene3Di1.20.840.10, 1 hit
3.30.70.470, 1 hit
3.90.390.10, 1 hit
InterProiView protein in InterPro
IPR016212 Me_CoM_Rdtase_asu
IPR008924 Me_CoM_Rdtase_asu/bsu_C
IPR009047 Me_CoM_Rdtase_asu_C
IPR003183 Me_CoM_Rdtase_asu_N
IPR015811 Me_CoM_Rdtase_asu_N_sub1
IPR015823 Me_CoM_Rdtase_asu_N_sub2
IPR009024 Me_CoM_Rdtase_Fd-like_fold
PfamiView protein in Pfam
PF02249 MCR_alpha, 1 hit
PF02745 MCR_alpha_N, 1 hit
PIRSFiPIRSF000262 MCR_alpha, 1 hit
SUPFAMiSSF48081 SSF48081, 1 hit
SSF55088 SSF55088, 1 hit
TIGRFAMsiTIGR03256 met_CoM_red_alp, 1 hit

Sequencei

Sequence statusi: Complete.

Q49605-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSAEEKLFM KALKEKFEES PEEKYTKFYI FGGWKQSERK KEFKEWADKI
60 70 80 90 100
VEERGVPHYN PDIGVPLGQR KLMSYQVSGT DVFVEGDDLH FVNNAAMQQM
110 120 130 140 150
WDDIRRTVIV GMDTAHRVLE RRLGKEVTPE TINEYMETLN HALPGGAVVQ
160 170 180 190 200
EHMVEIHPGL TWDCYAKIIT GDLELADEID DKFLIDIEKL FPEEQAEQLI
210 220 230 240 250
KAIGNRTYQV CRMPTIVGHV CDGATMYRWA AMQIAMSFIC AYKIAAGEAA
260 270 280 290 300
VSDFAFASKH AEVINMGEML PARRARGENE PGGVPFGVLA DCVQTMRKYP
310 320 330 340 350
DDPAKVALEV IAAGAMLYDQ IWLGSYMSGG VGFTQYATAV YTDNILDDYV
360 370 380 390 400
YYGLEYVEDK YGIAEAEPSM DVVKDVATEV TLYGLEQYER YPAAMETHFG
410 420 430 440 450
GSQRAAVCAA AAGCSTAFAT GHAQAGLNGW YLSQILHKEG HGRLGFYGYA
460 470 480 490 500
LQDQCGAANS LSVRSDEGLP LELRGPNYPN YAMNVGHLGE YAGIVQAAHA
510 520 530 540 550
ARGDAFCVHP VIKVAFADEN LVFDFTEPRK EFAKGALREF EPAGERDLIV

PAE
Length:553
Mass (Da):61,384
Last modified:April 16, 2002 - v2
Checksum:iF26582E0DCF2A434
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti90H → T in AAB02003 (PubMed:8863453).Curated1
Sequence conflicti342T → P in AAB02003 (PubMed:8863453).Curated1
Sequence conflicti441H → Q in AAB02003 (PubMed:8863453).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U57340 Genomic DNA Translation: AAB02003.1
AE009439 Genomic DNA Translation: AAM01870.1

Genome annotation databases

EnsemblBacteriaiAAM01870; AAM01870; MK0655
KEGGimka:MK0655
PATRICifig|190192.8.peg.694

Similar proteinsi

Entry informationi

Entry nameiMCRA_METKA
AccessioniPrimary (citable) accession number: Q49605
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: April 16, 2002
Last modified: May 23, 2018
This is version 122 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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