Q494E8 (ISPD_BLOPB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
EC=2.7.7.60

Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase
MEP cytidylyltransferase
Short name=MCT

Gene names

Name:	ispD
Ordered Locus Names:	BPEN_171

Organism

Blochmannia pennsylvanicus (strain BPEN) [Complete proteome] [HAMAP]

Taxonomic identifier

291272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › ant endosymbionts › Candidatus Blochmannia

Protein attributes

Sequence length	235 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108
Subunit structure	Homodimer By similarity. HAMAP MF_00108
Sequence similarities	Belongs to the IspD family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 235	235	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_00108		PRO_0000237775
Sites
	Site	21	1	Transition state stabilizer By similarity
	Site	28	1	Transition state stabilizer By similarity
	Site	158	1	Positions MEP for the nucleophilic attack By similarity
	Site	214	1	Positions MEP for the nucleophilic attack By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q494E8 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: BE656C9F62D8FDD8	FASTA	235	26,844
	10 20 30 40 50 60 MLKKKIPYIT AILPAAGTGK RMKSLLPKQY CTIGDKTLIE HSMNALLCQS CIRHCIVVIN 70 80 90 100 110 120 ARDHWFRQLS ISYDPRVSVV VGGHTRADSV MAGLQHVKKS VWVIVHDAVR PCLHHEDLLR 130 140 150 160 170 180 LFEITKFSQV GGILAIPICN TIKRTYCGTN FIRYTVNREN LWHALTPQLF NYDLLKYCLK 190 200 210 220 230 KALKNRVTVT DEASALEYCG YKSIVIHGRS DNIKVTHQND LKLANFYLSK LYKKT

References

[1]

"Genome sequence of Blochmannia pennsylvanicus indicates parallel evolutionary trends among bacterial mutualists of insects." Degnan P.H., Lazarus A.B., Wernegreen J.J. Genome Res. 15:1023-1033(2005) [PubMed: 16077009] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BPEN.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000016 Genomic DNA. Translation: AAZ40811.1.
RefSeq	YP_277684.1. NC_007292.1.
3D structure databases
ProteinModelPortal	Q494E8.
ModBase	Search...
Protein-protein interaction databases
STRING	Q494E8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3563117.
GenomeReviews	Gene locus BPEN_171 in contig CP000016_GR.
KEGG	bpn:BPEN_171.
PATRIC	31965235. VBICanBlo110049_0167.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1211.
HOGENOM	HBG672839.
OMA	FPQGAIL.
PhylomeDB	Q494E8.
Enzyme and pathway databases
BioCyc	CBLO291272:BPEN_171-MONOMER.
Family and domain databases
HAMAP	MF_00108. IspD. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. [Graphical view]
KO	K00991.
Pfam	PF01128. IspD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00453. IspD. 1 hit.
PROSITE	PS01295. ISPD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISPD_BLOPB

Accession

Primary (citable) accession number: Q494E8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	September 13, 2005
Last modified:	January 25, 2012
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program