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Q494C1

- GLMU_BLOPB

UniProt

Q494C1 - GLMU_BLOPB

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Protein

Bifunctional protein GlmU

Gene

glmU

Organism
Blochmannia pennsylvanicus (strain BPEN)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc). The C-terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N-acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5-triphosphate), a reaction catalyzed by the N-terminal domain.UniRule annotation

Catalytic activityi

Acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate.UniRule annotation
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation

Cofactori

Binds 1 magnesium ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei25 – 251UDP-GlcNAcUniRule annotation
Binding sitei80 – 801UDP-GlcNAcUniRule annotation
Metal bindingi109 – 1091MagnesiumUniRule annotation
Binding sitei144 – 1441UDP-GlcNAc; via amide nitrogenUniRule annotation
Binding sitei159 – 1591UDP-GlcNAcUniRule annotation
Metal bindingi233 – 2331MagnesiumUniRule annotation
Binding sitei233 – 2331UDP-GlcNAcUniRule annotation
Binding sitei339 – 3391Acetyl-CoA; amide nitrogenUniRule annotation
Binding sitei357 – 3571Acetyl-CoAUniRule annotation
Active sitei369 – 3691Proton acceptorUniRule annotation
Binding sitei372 – 3721Acetyl-CoAUniRule annotation
Binding sitei383 – 3831Acetyl-CoAUniRule annotation
Binding sitei429 – 4291Acetyl-CoA; via amide nitrogenUniRule annotation
Binding sitei446 – 4461Acetyl-CoAUniRule annotation

GO - Molecular functioni

  1. glucosamine-1-phosphate N-acetyltransferase activity Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. UDP-N-acetylglucosamine diphosphorylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cell morphogenesis Source: UniProtKB-HAMAP
  2. cell wall organization Source: UniProtKB-KW
  3. lipid A biosynthetic process Source: UniProtKB-UniPathway
  4. lipopolysaccharide biosynthetic process Source: InterPro
  5. peptidoglycan biosynthetic process Source: UniProtKB-HAMAP
  6. regulation of cell shape Source: UniProtKB-KW
  7. UDP-N-acetylglucosamine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciBPEN291272:GJ9N-16-MONOMER.
UniPathwayiUPA00113; UER00532.
UPA00113; UER00533.
UPA00973.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein GlmUUniRule annotation
Including the following 2 domains:
UDP-N-acetylglucosamine pyrophosphorylaseUniRule annotation (EC:2.7.7.23UniRule annotation)
Alternative name(s):
N-acetylglucosamine-1-phosphate uridyltransferaseUniRule annotation
Glucosamine-1-phosphate N-acetyltransferaseUniRule annotation (EC:2.3.1.157UniRule annotation)
Gene namesi
Name:glmUUniRule annotation
Ordered Locus Names:BPEN_010
OrganismiBlochmannia pennsylvanicus (strain BPEN)
Taxonomic identifieri291272 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeant endosymbiontsCandidatus Blochmannia
ProteomesiUP000007794: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 462462Bifunctional protein GlmUPRO_0000233739Add
BLAST

Interactioni

Subunit structurei

Homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGi291272.BPEN_010.

Structurei

3D structure databases

ProteinModelPortaliQ494C1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 235235PyrophosphorylaseUniRule annotationAdd
BLAST
Regioni11 – 144UDP-GlcNAc bindingUniRule annotation
Regioni85 – 862UDP-GlcNAc bindingUniRule annotation
Regioni107 – 1093UDP-GlcNAc bindingUniRule annotation
Regioni236 – 25621LinkerUniRule annotationAdd
BLAST
Regioni257 – 462206N-acetyltransferaseUniRule annotationAdd
BLAST
Regioni392 – 3932Acetyl-CoA bindingUniRule annotation

Sequence similaritiesi

In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family.UniRule annotation
In the C-terminal section; belongs to the transferase hexapeptide repeat family.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1207.
HOGENOMiHOG000283476.
KOiK04042.
OMAiDCVTNQD.
OrthoDBiEOG6Z6FQZ.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_01631. GlmU.
InterProiIPR005882. Bifunctional_GlmU.
IPR001451. Hexapep_transf.
IPR018357. Hexapep_transf_CS.
IPR025877. MobA-like_NTP_Trfase_dom.
IPR029044. Nucleotide-diphossugar_trans.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PfamiPF00132. Hexapep. 3 hits.
PF12804. NTP_transf_3. 1 hit.
[Graphical view]
SUPFAMiSSF51161. SSF51161. 1 hit.
SSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR01173. glmU. 1 hit.
PROSITEiPS00101. HEXAPEP_TRANSFERASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q494C1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSYINFSAII LAAGRGNRML SDTPKVLYQI GGKFMLQHLI DSVMQVGVSS
60 70 80 90 100
IYVVHGYKGE MIIKEINTNQ YKIPVYWILQ HDLTGTGDAV QRVLPFISDD
110 120 130 140 150
EEVLVLYGDV PFVSYKTLQR LHVIKSQCDI SMLTATLPNP KGYGRIVRNQ
160 170 180 190 200
EGSVVSIIEH DDIINDDQKK IKEVSTGIFI AISNHLKCWL STLTTHKSKN
210 220 230 240 250
EFYLTDIIQI AHQSGYSIHT MCPDDTFEIM GVNSKSDFVD LDKQYQQRKV
260 270 280 290 300
QCLLSSGLMI IDPNRFDLRG TLVHGKDVYI DINVIIEGHV SLGNRVKIGA
310 320 330 340 350
SCILKDTIVA DDVEIYPFSI IENTTIGFQS KVGPFVRLRP GTELKEKSHV
360 370 380 390 400
GNFVEIKNTR LGEQSKVKHL SYLGDAEIGN QVNIGAGTII CNYDGMMKHQ
410 420 430 440 450
TIIGDDVFIG ADSQLVAPIT IGKNVTIGAG TTVTRDVAAN ETIISRIRQF
460
SILNWKRLKN KK
Length:462
Mass (Da):51,550
Last modified:September 13, 2005 - v1
Checksum:i9E857B19A26FCFD2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000016 Genomic DNA. Translation: AAZ40660.1.
RefSeqiWP_011282566.1. NC_007292.1.
YP_277532.1. NC_007292.1.

Genome annotation databases

EnsemblBacteriaiAAZ40660; AAZ40660; BPEN_010.
GeneIDi3562625.
KEGGibpn:BPEN_010.
PATRICi31964906. VBICanBlo110049_0010.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000016 Genomic DNA. Translation: AAZ40660.1 .
RefSeqi WP_011282566.1. NC_007292.1.
YP_277532.1. NC_007292.1.

3D structure databases

ProteinModelPortali Q494C1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 291272.BPEN_010.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAZ40660 ; AAZ40660 ; BPEN_010 .
GeneIDi 3562625.
KEGGi bpn:BPEN_010.
PATRICi 31964906. VBICanBlo110049_0010.

Phylogenomic databases

eggNOGi COG1207.
HOGENOMi HOG000283476.
KOi K04042.
OMAi DCVTNQD.
OrthoDBi EOG6Z6FQZ.

Enzyme and pathway databases

UniPathwayi UPA00113 ; UER00532 .
UPA00113 ; UER00533 .
UPA00973 .
BioCyci BPEN291272:GJ9N-16-MONOMER.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
HAMAPi MF_01631. GlmU.
InterProi IPR005882. Bifunctional_GlmU.
IPR001451. Hexapep_transf.
IPR018357. Hexapep_transf_CS.
IPR025877. MobA-like_NTP_Trfase_dom.
IPR029044. Nucleotide-diphossugar_trans.
IPR011004. Trimer_LpxA-like.
[Graphical view ]
Pfami PF00132. Hexapep. 3 hits.
PF12804. NTP_transf_3. 1 hit.
[Graphical view ]
SUPFAMi SSF51161. SSF51161. 1 hit.
SSF53448. SSF53448. 1 hit.
TIGRFAMsi TIGR01173. glmU. 1 hit.
PROSITEi PS00101. HEXAPEP_TRANSFERASES. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of Blochmannia pennsylvanicus indicates parallel evolutionary trends among bacterial mutualists of insects."
    Degnan P.H., Lazarus A.B., Wernegreen J.J.
    Genome Res. 15:1023-1033(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BPEN.

Entry informationi

Entry nameiGLMU_BLOPB
AccessioniPrimary (citable) accession number: Q494C1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: September 13, 2005
Last modified: October 29, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3