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Protein

Bifunctional protein GlmU

Gene

glmU

Organism
Blochmannia pennsylvanicus (strain BPEN)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc). The C-terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N-acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5-triphosphate), a reaction catalyzed by the N-terminal domain.UniRule annotation

Catalytic activityi

Acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate.UniRule annotation
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: UDP-N-acetyl-alpha-D-glucosamine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route II).UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Bifunctional protein GlmU (glmU)
This subpathway is part of the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route II), the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis and in Nucleotide-sugar biosynthesis.

Pathwayi: UDP-N-acetyl-alpha-D-glucosamine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Bifunctional protein GlmU (glmU)
This subpathway is part of the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate, the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis and in Nucleotide-sugar biosynthesis.

Pathwayi: LPS lipid A biosynthesis

This protein is involved in the pathway LPS lipid A biosynthesis, which is part of Bacterial outer membrane biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway LPS lipid A biosynthesis and in Bacterial outer membrane biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei25UDP-GlcNAcUniRule annotation1
Binding sitei80UDP-GlcNAcUniRule annotation1
Metal bindingi109MagnesiumUniRule annotation1
Binding sitei144UDP-GlcNAc; via amide nitrogenUniRule annotation1
Binding sitei159UDP-GlcNAcUniRule annotation1
Metal bindingi233MagnesiumUniRule annotation1
Binding sitei233UDP-GlcNAcUniRule annotation1
Binding sitei339UDP-GlcNAcUniRule annotation1
Binding sitei357UDP-GlcNAcUniRule annotation1
Active sitei369Proton acceptorUniRule annotation1
Binding sitei372UDP-GlcNAcUniRule annotation1
Binding sitei383UDP-GlcNAcUniRule annotation1
Binding sitei386Acetyl-CoA; via amide nitrogenUniRule annotation1
Binding sitei429Acetyl-CoA; via amide nitrogenUniRule annotation1
Binding sitei446Acetyl-CoAUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Multifunctional enzyme, Nucleotidyltransferase, Transferase
Biological processCell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciCBLO291272:G1G3Y-10-MONOMER
UniPathwayiUPA00113; UER00532
UPA00113; UER00533
UPA00973

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein GlmUUniRule annotation
Including the following 2 domains:
UDP-N-acetylglucosamine pyrophosphorylaseUniRule annotation (EC:2.7.7.23UniRule annotation)
Alternative name(s):
N-acetylglucosamine-1-phosphate uridyltransferaseUniRule annotation
Glucosamine-1-phosphate N-acetyltransferaseUniRule annotation (EC:2.3.1.157UniRule annotation)
Gene namesi
Name:glmUUniRule annotation
Ordered Locus Names:BPEN_010
OrganismiBlochmannia pennsylvanicus (strain BPEN)
Taxonomic identifieri291272 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeant endosymbiontsCandidatus Blochmannia
Proteomesi
  • UP000007794 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002337391 – 462Bifunctional protein GlmUAdd BLAST462

Interactioni

Subunit structurei

Homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGi291272.BPEN_010

Structurei

3D structure databases

ProteinModelPortaliQ494C1
SMRiQ494C1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 235PyrophosphorylaseUniRule annotationAdd BLAST235
Regioni11 – 14UDP-GlcNAc bindingUniRule annotation4
Regioni85 – 86UDP-GlcNAc bindingUniRule annotation2
Regioni107 – 109UDP-GlcNAc bindingUniRule annotation3
Regioni236 – 256LinkerUniRule annotationAdd BLAST21
Regioni257 – 462N-acetyltransferaseUniRule annotationAdd BLAST206
Regioni392 – 393Acetyl-CoA bindingUniRule annotation2

Sequence similaritiesi

In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family.UniRule annotation
In the C-terminal section; belongs to the transferase hexapeptide repeat family.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105CAJ Bacteria
COG1207 LUCA
HOGENOMiHOG000283476
KOiK04042
OMAiIEPQTHL
OrthoDBiPOG091H02I2

Family and domain databases

CDDicd03353 LbH_GlmU_C, 1 hit
Gene3Di3.90.550.10, 1 hit
HAMAPiMF_01631 GlmU, 1 hit
InterProiView protein in InterPro
IPR005882 Bifunctional_GlmU
IPR038009 GlmU_C_LbH
IPR001451 Hexapep
IPR018357 Hexapep_transf_CS
IPR025877 MobA-like_NTP_Trfase
IPR029044 Nucleotide-diphossugar_trans
IPR011004 Trimer_LpxA-like_sf
PfamiView protein in Pfam
PF00132 Hexapep, 2 hits
PF12804 NTP_transf_3, 1 hit
SUPFAMiSSF51161 SSF51161, 1 hit
SSF53448 SSF53448, 1 hit
TIGRFAMsiTIGR01173 glmU, 1 hit
PROSITEiView protein in PROSITE
PS00101 HEXAPEP_TRANSFERASES, 1 hit

Sequencei

Sequence statusi: Complete.

Q494C1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYINFSAII LAAGRGNRML SDTPKVLYQI GGKFMLQHLI DSVMQVGVSS
60 70 80 90 100
IYVVHGYKGE MIIKEINTNQ YKIPVYWILQ HDLTGTGDAV QRVLPFISDD
110 120 130 140 150
EEVLVLYGDV PFVSYKTLQR LHVIKSQCDI SMLTATLPNP KGYGRIVRNQ
160 170 180 190 200
EGSVVSIIEH DDIINDDQKK IKEVSTGIFI AISNHLKCWL STLTTHKSKN
210 220 230 240 250
EFYLTDIIQI AHQSGYSIHT MCPDDTFEIM GVNSKSDFVD LDKQYQQRKV
260 270 280 290 300
QCLLSSGLMI IDPNRFDLRG TLVHGKDVYI DINVIIEGHV SLGNRVKIGA
310 320 330 340 350
SCILKDTIVA DDVEIYPFSI IENTTIGFQS KVGPFVRLRP GTELKEKSHV
360 370 380 390 400
GNFVEIKNTR LGEQSKVKHL SYLGDAEIGN QVNIGAGTII CNYDGMMKHQ
410 420 430 440 450
TIIGDDVFIG ADSQLVAPIT IGKNVTIGAG TTVTRDVAAN ETIISRIRQF
460
SILNWKRLKN KK
Length:462
Mass (Da):51,550
Last modified:September 13, 2005 - v1
Checksum:i9E857B19A26FCFD2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000016 Genomic DNA Translation: AAZ40660.1
RefSeqiWP_011282566.1, NC_007292.1

Genome annotation databases

EnsemblBacteriaiAAZ40660; AAZ40660; BPEN_010
KEGGibpn:BPEN_010

Similar proteinsi

Entry informationi

Entry nameiGLMU_BLOPB
AccessioniPrimary (citable) accession number: Q494C1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: September 13, 2005
Last modified: April 25, 2018
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health