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Q494C1

- GLMU_BLOPB

UniProt

Q494C1 - GLMU_BLOPB

Protein

Bifunctional protein GlmU

Gene

glmU

Organism
Blochmannia pennsylvanicus (strain BPEN)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 1 (13 Sep 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc). The C-terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N-acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5-triphosphate), a reaction catalyzed by the N-terminal domain.UniRule annotation

    Catalytic activityi

    Acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate.UniRule annotation
    UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation

    Cofactori

    Binds 1 magnesium ion per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei25 – 251UDP-GlcNAcUniRule annotation
    Binding sitei80 – 801UDP-GlcNAcUniRule annotation
    Metal bindingi109 – 1091MagnesiumUniRule annotation
    Binding sitei144 – 1441UDP-GlcNAc; via amide nitrogenUniRule annotation
    Binding sitei159 – 1591UDP-GlcNAcUniRule annotation
    Metal bindingi233 – 2331MagnesiumUniRule annotation
    Binding sitei233 – 2331UDP-GlcNAcUniRule annotation
    Binding sitei339 – 3391Acetyl-CoA; amide nitrogenUniRule annotation
    Binding sitei357 – 3571Acetyl-CoAUniRule annotation
    Active sitei369 – 3691Proton acceptorUniRule annotation
    Binding sitei372 – 3721Acetyl-CoAUniRule annotation
    Binding sitei383 – 3831Acetyl-CoAUniRule annotation
    Binding sitei429 – 4291Acetyl-CoA; via amide nitrogenUniRule annotation
    Binding sitei446 – 4461Acetyl-CoAUniRule annotation

    GO - Molecular functioni

    1. glucosamine-1-phosphate N-acetyltransferase activity Source: UniProtKB-HAMAP
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. UDP-N-acetylglucosamine diphosphorylase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. cell morphogenesis Source: UniProtKB-HAMAP
    2. lipid A biosynthetic process Source: UniProtKB-UniPathway
    3. lipopolysaccharide biosynthetic process Source: InterPro
    4. peptidoglycan biosynthetic process Source: UniProtKB-HAMAP
    5. regulation of cell shape Source: UniProtKB-KW
    6. UDP-N-acetylglucosamine biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Acyltransferase, Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciBPEN291272:GJ9N-16-MONOMER.
    UniPathwayiUPA00113; UER00532.
    UPA00113; UER00533.
    UPA00973.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional protein GlmUUniRule annotation
    Including the following 2 domains:
    UDP-N-acetylglucosamine pyrophosphorylaseUniRule annotation (EC:2.7.7.23UniRule annotation)
    Alternative name(s):
    N-acetylglucosamine-1-phosphate uridyltransferaseUniRule annotation
    Glucosamine-1-phosphate N-acetyltransferaseUniRule annotation (EC:2.3.1.157UniRule annotation)
    Gene namesi
    Name:glmUUniRule annotation
    Ordered Locus Names:BPEN_010
    OrganismiBlochmannia pennsylvanicus (strain BPEN)
    Taxonomic identifieri291272 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeant endosymbiontsCandidatus Blochmannia
    ProteomesiUP000007794: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 462462Bifunctional protein GlmUPRO_0000233739Add
    BLAST

    Interactioni

    Subunit structurei

    Homotrimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi291272.BPEN_010.

    Structurei

    3D structure databases

    ProteinModelPortaliQ494C1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 235235PyrophosphorylaseUniRule annotationAdd
    BLAST
    Regioni11 – 144UDP-GlcNAc bindingUniRule annotation
    Regioni85 – 862UDP-GlcNAc bindingUniRule annotation
    Regioni107 – 1093UDP-GlcNAc bindingUniRule annotation
    Regioni236 – 25621LinkerUniRule annotationAdd
    BLAST
    Regioni257 – 462206N-acetyltransferaseUniRule annotationAdd
    BLAST
    Regioni392 – 3932Acetyl-CoA bindingUniRule annotation

    Sequence similaritiesi

    In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family.UniRule annotation
    In the C-terminal section; belongs to the transferase hexapeptide repeat family.UniRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG1207.
    HOGENOMiHOG000283476.
    KOiK04042.
    OMAiDCVTNQD.
    OrthoDBiEOG6Z6FQZ.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    HAMAPiMF_01631. GlmU.
    InterProiIPR005882. Bifunctional_GlmU.
    IPR001451. Hexapep_transf.
    IPR018357. Hexapep_transf_CS.
    IPR025877. MobA-like_NTP_Trfase_dom.
    IPR029044. Nucleotide-diphossugar_trans.
    IPR011004. Trimer_LpxA-like.
    [Graphical view]
    PfamiPF00132. Hexapep. 3 hits.
    PF12804. NTP_transf_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF51161. SSF51161. 1 hit.
    SSF53448. SSF53448. 1 hit.
    TIGRFAMsiTIGR01173. glmU. 1 hit.
    PROSITEiPS00101. HEXAPEP_TRANSFERASES. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q494C1-1 [UniParc]FASTAAdd to Basket

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    MSYINFSAII LAAGRGNRML SDTPKVLYQI GGKFMLQHLI DSVMQVGVSS    50
    IYVVHGYKGE MIIKEINTNQ YKIPVYWILQ HDLTGTGDAV QRVLPFISDD 100
    EEVLVLYGDV PFVSYKTLQR LHVIKSQCDI SMLTATLPNP KGYGRIVRNQ 150
    EGSVVSIIEH DDIINDDQKK IKEVSTGIFI AISNHLKCWL STLTTHKSKN 200
    EFYLTDIIQI AHQSGYSIHT MCPDDTFEIM GVNSKSDFVD LDKQYQQRKV 250
    QCLLSSGLMI IDPNRFDLRG TLVHGKDVYI DINVIIEGHV SLGNRVKIGA 300
    SCILKDTIVA DDVEIYPFSI IENTTIGFQS KVGPFVRLRP GTELKEKSHV 350
    GNFVEIKNTR LGEQSKVKHL SYLGDAEIGN QVNIGAGTII CNYDGMMKHQ 400
    TIIGDDVFIG ADSQLVAPIT IGKNVTIGAG TTVTRDVAAN ETIISRIRQF 450
    SILNWKRLKN KK 462
    Length:462
    Mass (Da):51,550
    Last modified:September 13, 2005 - v1
    Checksum:i9E857B19A26FCFD2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000016 Genomic DNA. Translation: AAZ40660.1.
    RefSeqiYP_277532.1. NC_007292.1.

    Genome annotation databases

    EnsemblBacteriaiAAZ40660; AAZ40660; BPEN_010.
    GeneIDi3562625.
    KEGGibpn:BPEN_010.
    PATRICi31964906. VBICanBlo110049_0010.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000016 Genomic DNA. Translation: AAZ40660.1 .
    RefSeqi YP_277532.1. NC_007292.1.

    3D structure databases

    ProteinModelPortali Q494C1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 291272.BPEN_010.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAZ40660 ; AAZ40660 ; BPEN_010 .
    GeneIDi 3562625.
    KEGGi bpn:BPEN_010.
    PATRICi 31964906. VBICanBlo110049_0010.

    Phylogenomic databases

    eggNOGi COG1207.
    HOGENOMi HOG000283476.
    KOi K04042.
    OMAi DCVTNQD.
    OrthoDBi EOG6Z6FQZ.

    Enzyme and pathway databases

    UniPathwayi UPA00113 ; UER00532 .
    UPA00113 ; UER00533 .
    UPA00973 .
    BioCyci BPEN291272:GJ9N-16-MONOMER.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    HAMAPi MF_01631. GlmU.
    InterProi IPR005882. Bifunctional_GlmU.
    IPR001451. Hexapep_transf.
    IPR018357. Hexapep_transf_CS.
    IPR025877. MobA-like_NTP_Trfase_dom.
    IPR029044. Nucleotide-diphossugar_trans.
    IPR011004. Trimer_LpxA-like.
    [Graphical view ]
    Pfami PF00132. Hexapep. 3 hits.
    PF12804. NTP_transf_3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51161. SSF51161. 1 hit.
    SSF53448. SSF53448. 1 hit.
    TIGRFAMsi TIGR01173. glmU. 1 hit.
    PROSITEi PS00101. HEXAPEP_TRANSFERASES. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of Blochmannia pennsylvanicus indicates parallel evolutionary trends among bacterial mutualists of insects."
      Degnan P.H., Lazarus A.B., Wernegreen J.J.
      Genome Res. 15:1023-1033(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: BPEN.

    Entry informationi

    Entry nameiGLMU_BLOPB
    AccessioniPrimary (citable) accession number: Q494C1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 2, 2006
    Last sequence update: September 13, 2005
    Last modified: October 1, 2014
    This is version 66 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3