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Protein

Bifunctional protein GlmU

Gene

glmU

Organism
Blochmannia pennsylvanicus (strain BPEN)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc). The C-terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N-acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5-triphosphate), a reaction catalyzed by the N-terminal domain.UniRule annotation

Catalytic activityi

Acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate.UniRule annotation
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathway:iUDP-N-acetyl-alpha-D-glucosamine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route II).UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Bifunctional protein GlmU (glmU)
This subpathway is part of the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route II), the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis and in Nucleotide-sugar biosynthesis.

Pathway:iUDP-N-acetyl-alpha-D-glucosamine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Bifunctional protein GlmU (glmU)
This subpathway is part of the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate, the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis and in Nucleotide-sugar biosynthesis.

Pathway:iLPS lipid A biosynthesis

This protein is involved in the pathway LPS lipid A biosynthesis, which is part of Bacterial outer membrane biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway LPS lipid A biosynthesis and in Bacterial outer membrane biogenesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei25 – 251UDP-GlcNAcUniRule annotation
Binding sitei80 – 801UDP-GlcNAcUniRule annotation
Metal bindingi109 – 1091MagnesiumUniRule annotation
Binding sitei144 – 1441UDP-GlcNAc; via amide nitrogenUniRule annotation
Binding sitei159 – 1591UDP-GlcNAcUniRule annotation
Metal bindingi233 – 2331MagnesiumUniRule annotation
Binding sitei233 – 2331UDP-GlcNAcUniRule annotation
Binding sitei339 – 3391Acetyl-CoA; amide nitrogenUniRule annotation
Binding sitei357 – 3571Acetyl-CoAUniRule annotation
Active sitei369 – 3691Proton acceptorUniRule annotation
Binding sitei372 – 3721Acetyl-CoAUniRule annotation
Binding sitei383 – 3831Acetyl-CoAUniRule annotation
Binding sitei429 – 4291Acetyl-CoA; via amide nitrogenUniRule annotation
Binding sitei446 – 4461Acetyl-CoAUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciBPEN291272:GJ9N-16-MONOMER.
UniPathwayiUPA00113; UER00532.
UPA00113; UER00533.
UPA00973.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein GlmUUniRule annotation
Including the following 2 domains:
UDP-N-acetylglucosamine pyrophosphorylaseUniRule annotation (EC:2.7.7.23UniRule annotation)
Alternative name(s):
N-acetylglucosamine-1-phosphate uridyltransferaseUniRule annotation
Glucosamine-1-phosphate N-acetyltransferaseUniRule annotation (EC:2.3.1.157UniRule annotation)
Gene namesi
Name:glmUUniRule annotation
Ordered Locus Names:BPEN_010
OrganismiBlochmannia pennsylvanicus (strain BPEN)
Taxonomic identifieri291272 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeant endosymbiontsCandidatus Blochmannia
ProteomesiUP000007794 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 462462Bifunctional protein GlmUPRO_0000233739Add
BLAST

Interactioni

Subunit structurei

Homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGi291272.BPEN_010.

Structurei

3D structure databases

ProteinModelPortaliQ494C1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 235235PyrophosphorylaseUniRule annotationAdd
BLAST
Regioni11 – 144UDP-GlcNAc bindingUniRule annotation
Regioni85 – 862UDP-GlcNAc bindingUniRule annotation
Regioni107 – 1093UDP-GlcNAc bindingUniRule annotation
Regioni236 – 25621LinkerUniRule annotationAdd
BLAST
Regioni257 – 462206N-acetyltransferaseUniRule annotationAdd
BLAST
Regioni392 – 3932Acetyl-CoA bindingUniRule annotation

Sequence similaritiesi

In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family.UniRule annotation
In the C-terminal section; belongs to the transferase hexapeptide repeat family.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1207.
HOGENOMiHOG000283476.
KOiK04042.
OMAiFAHARPK.
OrthoDBiEOG6Z6FQZ.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_01631. GlmU.
InterProiIPR005882. Bifunctional_GlmU.
IPR001451. Hexapep_transf.
IPR018357. Hexapep_transf_CS.
IPR025877. MobA-like_NTP_Trfase_dom.
IPR029044. Nucleotide-diphossugar_trans.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PfamiPF00132. Hexapep. 3 hits.
PF12804. NTP_transf_3. 1 hit.
[Graphical view]
SUPFAMiSSF51161. SSF51161. 1 hit.
SSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR01173. glmU. 1 hit.
PROSITEiPS00101. HEXAPEP_TRANSFERASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q494C1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYINFSAII LAAGRGNRML SDTPKVLYQI GGKFMLQHLI DSVMQVGVSS
60 70 80 90 100
IYVVHGYKGE MIIKEINTNQ YKIPVYWILQ HDLTGTGDAV QRVLPFISDD
110 120 130 140 150
EEVLVLYGDV PFVSYKTLQR LHVIKSQCDI SMLTATLPNP KGYGRIVRNQ
160 170 180 190 200
EGSVVSIIEH DDIINDDQKK IKEVSTGIFI AISNHLKCWL STLTTHKSKN
210 220 230 240 250
EFYLTDIIQI AHQSGYSIHT MCPDDTFEIM GVNSKSDFVD LDKQYQQRKV
260 270 280 290 300
QCLLSSGLMI IDPNRFDLRG TLVHGKDVYI DINVIIEGHV SLGNRVKIGA
310 320 330 340 350
SCILKDTIVA DDVEIYPFSI IENTTIGFQS KVGPFVRLRP GTELKEKSHV
360 370 380 390 400
GNFVEIKNTR LGEQSKVKHL SYLGDAEIGN QVNIGAGTII CNYDGMMKHQ
410 420 430 440 450
TIIGDDVFIG ADSQLVAPIT IGKNVTIGAG TTVTRDVAAN ETIISRIRQF
460
SILNWKRLKN KK
Length:462
Mass (Da):51,550
Last modified:September 13, 2005 - v1
Checksum:i9E857B19A26FCFD2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000016 Genomic DNA. Translation: AAZ40660.1.
RefSeqiWP_011282566.1. NC_007292.1.

Genome annotation databases

EnsemblBacteriaiAAZ40660; AAZ40660; BPEN_010.
KEGGibpn:BPEN_010.
PATRICi31964906. VBICanBlo110049_0010.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000016 Genomic DNA. Translation: AAZ40660.1.
RefSeqiWP_011282566.1. NC_007292.1.

3D structure databases

ProteinModelPortaliQ494C1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi291272.BPEN_010.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAZ40660; AAZ40660; BPEN_010.
KEGGibpn:BPEN_010.
PATRICi31964906. VBICanBlo110049_0010.

Phylogenomic databases

eggNOGiCOG1207.
HOGENOMiHOG000283476.
KOiK04042.
OMAiFAHARPK.
OrthoDBiEOG6Z6FQZ.

Enzyme and pathway databases

UniPathwayiUPA00113; UER00532.
UPA00113; UER00533.
UPA00973.
BioCyciBPEN291272:GJ9N-16-MONOMER.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_01631. GlmU.
InterProiIPR005882. Bifunctional_GlmU.
IPR001451. Hexapep_transf.
IPR018357. Hexapep_transf_CS.
IPR025877. MobA-like_NTP_Trfase_dom.
IPR029044. Nucleotide-diphossugar_trans.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PfamiPF00132. Hexapep. 3 hits.
PF12804. NTP_transf_3. 1 hit.
[Graphical view]
SUPFAMiSSF51161. SSF51161. 1 hit.
SSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR01173. glmU. 1 hit.
PROSITEiPS00101. HEXAPEP_TRANSFERASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Blochmannia pennsylvanicus indicates parallel evolutionary trends among bacterial mutualists of insects."
    Degnan P.H., Lazarus A.B., Wernegreen J.J.
    Genome Res. 15:1023-1033(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BPEN.

Entry informationi

Entry nameiGLMU_BLOPB
AccessioniPrimary (citable) accession number: Q494C1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: September 13, 2005
Last modified: July 22, 2015
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.