ID   NAOX_MYCGE              Reviewed;         478 AA.
AC   Q49408; Q49235;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   05-MAY-2009, entry version 54.
DE   RecName: Full=Probable NADH oxidase;
DE            Short=NOXase;
DE            EC=1.6.99.3;
GN   Name=nox; OrderedLocusNames=MG275;
OS   Mycoplasma genitalium.
OC   Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mycoplasma.
OX   NCBI_TaxID=2097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 33530 / G-37 / NCTC 10195;
RX   MEDLINE=96026346; PubMed=7569993; DOI=10.1126/science.270.5235.397;
RA   Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A.,
RA   Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M.,
RA   Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L.,
RA   Nguyen D.T., Utterback T.R., Saudek D.M., Phillips C.A., Merrick J.M.,
RA   Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S.,
RA   Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C.;
RT   "The minimal gene complement of Mycoplasma genitalium.";
RL   Science 270:397-403(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-28.
RC   STRAIN=ATCC 33530 / G-37 / NCTC 10195;
RX   MEDLINE=94075230; PubMed=8253680;
RA   Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III;
RT   "A survey of the Mycoplasma genitalium genome by using random
RT   sequencing.";
RL   J. Bacteriol. 175:7918-7930(1993).
CC   -!- FUNCTION: Catalyzes the four-electron reduction of molecular
CC       oxygen to water (By similarity).
CC   -!- CATALYTIC ACTIVITY: NADH + acceptor = NAD(+) + reduced acceptor.
CC   -!- COFACTOR: Binds 1 FAD per subunit (By similarity).
CC   -!- SIMILARITY: Belongs to the class-III pyridine nucleotide-disulfide
CC       oxidoreductase family.
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DR   EMBL; L43967; AAC71497.1; -; Genomic_DNA.
DR   EMBL; U01786; AAD10607.1; ALT_INIT; Genomic_DNA.
DR   PIR; D64230; D64230.
DR   RefSeq; NP_072942.2; -.
DR   HSSP; P37062; 1NHP.
DR   GeneID; 875557; -.
DR   GenomeReviews; L43967_GR; MG275.
DR   KEGG; mge:MG_275; -.
DR   TIGR; MG275; -.
DR   HOGENOM; Q49408; -.
DR   BioCyc; MGEN243273:MG_275-MON; -.
DR   BRENDA; 1.6.99.3; 110.
DR   GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR   GO; GO:0050660; F:FAD binding; IEA:InterPro.
DR   GO; GO:0003954; F:NADH dehydrogenase activity; IEA:EC.
DR   GO; GO:0045454; P:cell redox homeostasis; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR013027; FAD_pyr_nucl-diS_OxRdtase.
DR   InterPro; IPR004099; Pyr_nucl-diS_OxRdtase_dimer.
DR   InterPro; IPR001327; Pyr_OxRdtase_NAD_bd.
DR   Gene3D; G3DSA:3.30.390.30; Pyr_redox_dim; 1.
DR   Pfam; PF00070; Pyr_redox; 1.
DR   Pfam; PF07992; Pyr_redox_2; 1.
DR   Pfam; PF02852; Pyr_redox_dim; 1.
DR   PRINTS; PR00368; FADPNR.
DR   ProDom; PD000139; FAD_pyr_redox; 1.
PE   3: Inferred from homology;
KW   Complete proteome; FAD; Flavoprotein; NAD; Oxidation; Oxidoreductase;
KW   Redox-active center.
FT   CHAIN         1    478       Probable NADH oxidase.
FT                                /FTId=PRO_0000184702.
FT   NP_BIND       8     12       FAD (By similarity).
FT   NP_BIND     111    114       FAD (By similarity).
FT   NP_BIND     170    185       NAD (By similarity).
FT   NP_BIND     295    305       FAD (By similarity).
FT   ACT_SITE     11     11       Proton acceptor (By similarity).
FT   ACT_SITE     43     43       Redox-active.
FT   BINDING      43     43       FAD (By similarity).
FT   BINDING     197    197       NAD (By similarity).
FT   BINDING     264    264       NAD; via amide nitrogen (By similarity).
FT   BINDING     323    323       FAD; via amide nitrogen (By similarity).
FT   MOD_RES      43     43       Cysteine sulfenic acid (-SOH) (By
FT                                similarity).
FT   CONFLICT     28     28       K -> P (in Ref. 2; AAD10607).
SQ   SEQUENCE   478 AA;  53173 MW;  A96A76F64DEBD0C8 CRC64;
     MKKVIVIGIN HAGTSFIRTL LSKSKDFKVN AYDRNTNISF LGCGIALAVS GVVKNTDDLF
     YSNPEELKQM GANIFMSHDV TNIDLIKKQV TVRDLTSNKE FTDQFDQLVI ASGAWPICMN
     VENKVTHKPL EFNYTDKYCG NVKNLISCKL YQHALTLIDS FRKDKTIKSV AIVGSGYIGL
     ELAEAAWLCK KQVTVIDLLD KPAGNNFDHE FTDELEKVMQ KDGLKLMMGC SVKGFVVDST
     NNVVKGVETD KGIVNADLVN QSIGFRPSTK FVPKDQNFEF IHNGSIKVNE FLQALNHKDV
     YVIGGCAAIY NAASEQYENI DLATNAVKSG LVAAMHIIGS NQVKLQSIVG TNALHIFGLN
     LAACGLTEQR AKKLGFDVGI SVVDDNDRPE FMGSYDKVRF KLVYDKKTLR ILGAQLLSWN
     TNHSEIIFYI ALAIQKQMLL TELGLVDVYF LPHYNKPFNF VLATVLQALG FSYYIPKK
//