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Protein

Probable NADH oxidase

Gene

nox

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the four-electron reduction of molecular oxygen to water.By similarity

Catalytic activityi

NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

FADBy similarityNote: Binds 1 FAD per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei11Proton acceptorBy similarity1
Active sitei43Redox-active1
Binding sitei43FADBy similarity1
Binding sitei197NADBy similarity1
Binding sitei264NAD; via amide nitrogenBy similarity1
Binding sitei323FAD; via amide nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi8 – 12FADBy similarity5
Nucleotide bindingi111 – 114FADBy similarity4
Nucleotide bindingi170 – 185NADBy similarityAdd BLAST16
Nucleotide bindingi295 – 305FADBy similarityAdd BLAST11

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandFAD, Flavoprotein, NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Probable NADH oxidase (EC:1.6.99.3)
Short name:
NOXase
Gene namesi
Name:nox
Ordered Locus Names:MG275
OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Taxonomic identifieri243273 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
Proteomesi
  • UP000000807 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Disruption phenotypei

Probably essential, it was not disrupted in a global transposon mutagenesis study.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001847021 – 478Probable NADH oxidaseAdd BLAST478

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei43Cysteine sulfenic acid (-SOH)By similarity1

Keywords - PTMi

Oxidation

Interactioni

Protein-protein interaction databases

STRINGi243273.MgenG_010200002659

Structurei

3D structure databases

ProteinModelPortaliQ49408
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Redox-active center

Phylogenomic databases

eggNOGiENOG4107QMW Bacteria
COG0446 LUCA
OrthoDBiPOG091H02KF

Family and domain databases

Gene3Di3.30.390.30, 1 hit
3.50.50.60, 5 hits
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR023753 FAD/NAD-binding_dom
IPR016156 FAD/NAD-linked_Rdtase_dimer_sf
IPR004099 Pyr_nucl-diS_OxRdtase_dimer
PfamiView protein in Pfam
PF07992 Pyr_redox_2, 1 hit
PF02852 Pyr_redox_dim, 1 hit
SUPFAMiSSF51905 SSF51905, 1 hit
SSF55424 SSF55424, 1 hit

Sequencei

Sequence statusi: Complete.

Q49408-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKVIVIGIN HAGTSFIRTL LSKSKDFKVN AYDRNTNISF LGCGIALAVS
60 70 80 90 100
GVVKNTDDLF YSNPEELKQM GANIFMSHDV TNIDLIKKQV TVRDLTSNKE
110 120 130 140 150
FTDQFDQLVI ASGAWPICMN VENKVTHKPL EFNYTDKYCG NVKNLISCKL
160 170 180 190 200
YQHALTLIDS FRKDKTIKSV AIVGSGYIGL ELAEAAWLCK KQVTVIDLLD
210 220 230 240 250
KPAGNNFDHE FTDELEKVMQ KDGLKLMMGC SVKGFVVDST NNVVKGVETD
260 270 280 290 300
KGIVNADLVI QSIGFRPSTK FVPKDQNFEF IHNGSIKVNE FLQALNHKDV
310 320 330 340 350
YVIGGCAAIY NAASEQYENI DLATNAVKSG LVAAMHIIGS NQVKLQSIVG
360 370 380 390 400
TNALHIFGLN LAACGLTEQR AKKLGFDVGI SVVDDNDRPE FMGSYDKVRF
410 420 430 440 450
KLVYDKKTLR ILGAQLLSWN TNHSEIIFYI ALAIQKQMLL TELGLVDVYF
460 470
LPHYNKPFNF VLATVLQALG FSYYIPKK
Length:478
Mass (Da):53,172
Last modified:January 31, 2018 - v2
Checksum:iDD5034951CA9B0BB
GO

Sequence cautioni

The sequence AAD10607 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti28K → P in AAD10607 (PubMed:8253680).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA Translation: AAC71497.2
U01786 Genomic DNA Translation: AAD10607.1 Different initiation.
PIRiD64230
RefSeqiWP_009885907.1, NZ_AAGX01000009.1

Genome annotation databases

EnsemblBacteriaiAAC71497; AAC71497; MG_275
KEGGimge:MG_275

Similar proteinsi

Entry informationi

Entry nameiNAOX_MYCGE
AccessioniPrimary (citable) accession number: Q49408
Secondary accession number(s): Q49235
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 31, 2018
Last modified: March 28, 2018
This is version 103 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health