Reviewed, UniProtKB/Swiss-Prot Q49408 (NAOX_MYCGE)

Names and origin

Protein names

Recommended name:
    Probable NADH oxidase
      Short name=NOXase
    EC=1.6.99.3

Gene names

Name:	nox
Ordered Locus Names:	MG275

Organism

Mycoplasma genitalium [Complete proteome] [HAMAP]

Taxonomic identifier

2097 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	478 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the four-electron reduction of molecular oxygen to water By similarity.
Catalytic activity	NADH + acceptor = NAD+ + reduced acceptor.
Cofactor	Binds 1 FAD per subunit By similarity.
Sequence similarities	Belongs to the class-III pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Domain	Redox-active center
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
PTM	Oxidation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 478	478	Probable NADH oxidase		PRO_0000184702
Regions
	Nucleotide binding	8 – 12	5	FAD By similarity
	Nucleotide binding	111 – 114	4	FAD By similarity
	Nucleotide binding	170 – 185	16	NAD By similarity
	Nucleotide binding	295 – 305	11	FAD By similarity
Sites
	Active site	11	1	Proton acceptor By similarity
	Active site	43	1	Redox-active
	Binding site	43	1	FAD By similarity
	Binding site	197	1	NAD By similarity
	Binding site	264	1	NAD; via amide nitrogen By similarity
	Binding site	323	1	FAD; via amide nitrogen By similarity
Amino acid modifications
	Modified residue	43	1	Cysteine sulfenic acid (-SOH) By similarity
Experimental info
	Sequence conflict	28	1	K → P in AAD10607. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q49408-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: A96A76F64DEBD0C8	FASTA	478	53,173
	10 20 30 40 50 60 MKKVIVIGIN HAGTSFIRTL LSKSKDFKVN AYDRNTNISF LGCGIALAVS GVVKNTDDLF 70 80 90 100 110 120 YSNPEELKQM GANIFMSHDV TNIDLIKKQV TVRDLTSNKE FTDQFDQLVI ASGAWPICMN 130 140 150 160 170 180 VENKVTHKPL EFNYTDKYCG NVKNLISCKL YQHALTLIDS FRKDKTIKSV AIVGSGYIGL 190 200 210 220 230 240 ELAEAAWLCK KQVTVIDLLD KPAGNNFDHE FTDELEKVMQ KDGLKLMMGC SVKGFVVDST 250 260 270 280 290 300 NNVVKGVETD KGIVNADLVN QSIGFRPSTK FVPKDQNFEF IHNGSIKVNE FLQALNHKDV 310 320 330 340 350 360 YVIGGCAAIY NAASEQYENI DLATNAVKSG LVAAMHIIGS NQVKLQSIVG TNALHIFGLN 370 380 390 400 410 420 LAACGLTEQR AKKLGFDVGI SVVDDNDRPE FMGSYDKVRF KLVYDKKTLR ILGAQLLSWN 430 440 450 460 470 TNHSEIIFYI ALAIQKQMLL TELGLVDVYF LPHYNKPFNF VLATVLQALG FSYYIPKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The minimal gene complement of Mycoplasma genitalium." Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. , Phillips C.A., Merrick J.M., Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S., Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C. Science 270:397-403(1995) [PubMed: 7569993] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.
[2]	"A survey of the Mycoplasma genitalium genome by using random sequencing." Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III J. Bacteriol. 175:7918-7930(1993) [PubMed: 8253680] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-28. Strain: ATCC 33530 / G-37 / NCTC 10195.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L43967 Genomic DNA. Translation: AAC71497.1. U01786 Genomic DNA. Translation: AAD10607.1. Different initiation.
PIR	D64230.
RefSeq	NP_072942.2.
3D structure databases
SMR	Q49408. Positions 1-464.
ModBase	Search...
Genome annotation databases
GeneID	875557.
GenomeReviews	Gene locus MG275 in contig L43967_GR.
KEGG	mge:MG_275.
TIGR	MG275.
Phylogenomic databases
HOGENOM	HBG535576.
Enzyme and pathway databases
BioCyc	MGEN243273:MG_275-MONOMER.
BRENDA	1.6.99.3. 110.
Family and domain databases
InterPro	IPR016156. FAD/NAD-linked_Rdtase_dimer. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR004099. Pyr_nucl-diS_OxRdtase_dimer. IPR001327. Pyr_OxRdtase_NAD_bd. [Graphical view]
Gene3D	G3DSA:3.30.390.30. Pyr_redox_dim. 1 hit.
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. PF02852. Pyr_redox_dim. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR.
ProtoNet	Search...

Entry information

Entry name

NAOX_MYCGE

Accession

Primary (citable) accession number: Q49408
Secondary accession number(s): Q49235

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	January 19, 2010
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)