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Protein

Probable NADH oxidase

Gene

nox

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the four-electron reduction of molecular oxygen to water.By similarity

Catalytic activityi

NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

FADBy similarityNote: Binds 1 FAD per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei11 – 111Proton acceptorBy similarity
Active sitei43 – 431Redox-active
Binding sitei43 – 431FADBy similarity
Binding sitei197 – 1971NADBy similarity
Binding sitei264 – 2641NAD; via amide nitrogenBy similarity
Binding sitei323 – 3231FAD; via amide nitrogenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi8 – 125FADBy similarity
Nucleotide bindingi111 – 1144FADBy similarity
Nucleotide bindingi170 – 18516NADBy similarityAdd
BLAST
Nucleotide bindingi295 – 30511FADBy similarityAdd
BLAST

GO - Molecular functioni

  1. flavin adenine dinucleotide binding Source: InterPro
  2. NADH dehydrogenase activity Source: UniProtKB-EC

GO - Biological processi

  1. cell redox homeostasis Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, NAD

Enzyme and pathway databases

BioCyciMGEN243273:GH2R-304-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable NADH oxidase (EC:1.6.99.3)
Short name:
NOXase
Gene namesi
Name:nox
Ordered Locus Names:MG275
OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Taxonomic identifieri243273 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000807: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 478478Probable NADH oxidasePRO_0000184702Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei43 – 431Cysteine sulfenic acid (-SOH)By similarity

Keywords - PTMi

Oxidation

Interactioni

Protein-protein interaction databases

STRINGi243273.MG_275.

Structurei

3D structure databases

ProteinModelPortaliQ49408.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Redox-active center

Phylogenomic databases

eggNOGiCOG0446.
KOiK03885.
OrthoDBiEOG6QVRCJ.

Family and domain databases

Gene3Di3.30.390.30. 1 hit.
InterProiIPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR013027. FAD_pyr_nucl-diS_OxRdtase.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
IPR001327. Pyr_OxRdtase_NAD-bd_dom.
[Graphical view]
PfamiPF00070. Pyr_redox. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
PF02852. Pyr_redox_dim. 1 hit.
[Graphical view]
PRINTSiPR00368. FADPNR.
SUPFAMiSSF55424. SSF55424. 1 hit.

Sequencei

Sequence statusi: Complete.

Q49408-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKVIVIGIN HAGTSFIRTL LSKSKDFKVN AYDRNTNISF LGCGIALAVS
60 70 80 90 100
GVVKNTDDLF YSNPEELKQM GANIFMSHDV TNIDLIKKQV TVRDLTSNKE
110 120 130 140 150
FTDQFDQLVI ASGAWPICMN VENKVTHKPL EFNYTDKYCG NVKNLISCKL
160 170 180 190 200
YQHALTLIDS FRKDKTIKSV AIVGSGYIGL ELAEAAWLCK KQVTVIDLLD
210 220 230 240 250
KPAGNNFDHE FTDELEKVMQ KDGLKLMMGC SVKGFVVDST NNVVKGVETD
260 270 280 290 300
KGIVNADLVN QSIGFRPSTK FVPKDQNFEF IHNGSIKVNE FLQALNHKDV
310 320 330 340 350
YVIGGCAAIY NAASEQYENI DLATNAVKSG LVAAMHIIGS NQVKLQSIVG
360 370 380 390 400
TNALHIFGLN LAACGLTEQR AKKLGFDVGI SVVDDNDRPE FMGSYDKVRF
410 420 430 440 450
KLVYDKKTLR ILGAQLLSWN TNHSEIIFYI ALAIQKQMLL TELGLVDVYF
460 470
LPHYNKPFNF VLATVLQALG FSYYIPKK
Length:478
Mass (Da):53,173
Last modified:November 1, 1997 - v1
Checksum:iA96A76F64DEBD0C8
GO

Sequence cautioni

The sequence AAD10607.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti28 – 281K → P in AAD10607 (PubMed:8253680).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC71497.1.
U01786 Genomic DNA. Translation: AAD10607.1. Different initiation.
PIRiD64230.
RefSeqiNP_072942.2. NC_000908.2.

Genome annotation databases

EnsemblBacteriaiAAC71497; AAC71497; MG_275.
GeneIDi875557.
KEGGimge:MG_275.
PATRICi20010050. VBIMycGen98045_0317.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC71497.1.
U01786 Genomic DNA. Translation: AAD10607.1. Different initiation.
PIRiD64230.
RefSeqiNP_072942.2. NC_000908.2.

3D structure databases

ProteinModelPortaliQ49408.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243273.MG_275.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC71497; AAC71497; MG_275.
GeneIDi875557.
KEGGimge:MG_275.
PATRICi20010050. VBIMycGen98045_0317.

Phylogenomic databases

eggNOGiCOG0446.
KOiK03885.
OrthoDBiEOG6QVRCJ.

Enzyme and pathway databases

BioCyciMGEN243273:GH2R-304-MONOMER.

Family and domain databases

Gene3Di3.30.390.30. 1 hit.
InterProiIPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR013027. FAD_pyr_nucl-diS_OxRdtase.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
IPR001327. Pyr_OxRdtase_NAD-bd_dom.
[Graphical view]
PfamiPF00070. Pyr_redox. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
PF02852. Pyr_redox_dim. 1 hit.
[Graphical view]
PRINTSiPR00368. FADPNR.
SUPFAMiSSF55424. SSF55424. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33530 / G-37 / NCTC 10195.
  2. "A survey of the Mycoplasma genitalium genome by using random sequencing."
    Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III
    J. Bacteriol. 175:7918-7930(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-28.
    Strain: ATCC 33530 / G-37 / NCTC 10195.

Entry informationi

Entry nameiNAOX_MYCGE
AccessioniPrimary (citable) accession number: Q49408
Secondary accession number(s): Q49235
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: January 7, 2015
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Mycoplasma genitalium
    Mycoplasma genitalium (strain G-37): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.