Q49405 (DPO3A_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA polymerase III subunit alpha
EC=2.7.7.7

Gene names

Name:	dnaE
Ordered Locus Names:	MG261

Organism

Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]

Taxonomic identifier

243273 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma › Mycoplasma genitalium

Protein attributes

Sequence length	874 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Subunit structure	DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the PolIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE subfamily.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 874	874	DNA polymerase III subunit alpha		PRO_0000103326

Sequences

	Sequence		Length	Mass (Da)
	Q49405 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 9B27C96A28A74909	FASTA	874	100,435
	10 20 30 40 50 60 MFVNLHTNSY YNFLNSALSP KKLVNLAIND QQKAVAITDP NLFGAVEFFI TCKQNNIKPI 70 80 90 100 110 120 IGLNLTVEYQ KNDVKLLLIA KSNKGFQTLN KIALIQQKLE INSLVDQLTD IAVIICSLTT 130 140 150 160 170 180 WKSTYKDVYQ AKGIEINQTP IAILANAVNC EKTNSDQVVL TVLKQMKQNQ TGKITTFDWD 190 200 210 220 230 240 LKQKLNQISI NENLKVKSEI QPFLDQKTAQ QLFSETELNN LNDLVNRCEL DLEHLKAASL 250 260 270 280 290 300 SLTDNDAAVL ESLCQTNLKQ FLDKNQDLNK KAYQLRLEKE LNVINKLNFA SYFLVVNDLV 310 320 330 340 350 360 NYAFKKDILI GSGRGSAVGS LVAFLLNITK IDPVQHQLIF ERFISTHRQD LPDIDIDIME 370 380 390 400 410 420 NKRAEMINYL FEKYGKENCA QIVTFQRFKT RSAVKEVAKL FNDYGISDMI LGVLPKDQTI 430 440 450 460 470 480 TFTDLKATED SALQLCLQQF GLIVELALAI VDFPRQSSIH ASGIVIASNS LIKTIPLLQL 490 500 510 520 530 540 DNNHFLTQVS MEWLSFFNLN KFDLLGLINL TMISDVITQI KPSNQTVNQF LNTISWTDQN 550 560 570 580 590 600 TFINLVNEDT LGIFQLESFG MKKLLVQIKP KTINQLAIVL ALYRPGAQDN INLFINRLHN 610 620 630 640 650 660 GYDQSDIDPR ILPIVKNTYG VLIFQEQIIN IVKVVANYSL EEADSFRRAI SKKDVKLIQK 670 680 690 700 710 720 NKRNFFERAV QNNFDLKTTT KIFSYIERFA NYGFNLSHAL GYALLSYWTA WLKTNYPVYF 730 740 750 760 770 780 YLWLLNHFQS SKDKQKLIIR TLEKSGIEIY PPLLNKAQPN SVIENKKIYL GLNLIKGIND 790 800 810 820 830 840 RYIQNLQKVQ HLIQTQNNLQ LTDVVSWCLD KTIGDIPLKD LLLLKTMGCF DFFEYTYDFN 850 860 870 DAKDFWIKSD HLLFTRMPLE KKDSNFWIKQ FFTN

References

[1]

"The minimal gene complement of Mycoplasma genitalium." Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. , Phillips C.A., Merrick J.M., Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S., Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C. Science 270:397-403(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L43967 Genomic DNA. Translation: AAC71482.1.
PIR	H64228.
RefSeq	NP_072927.1. NC_000908.2.
3D structure databases
ProteinModelPortal	Q49405.
ModBase	Search...
Protein-protein interaction databases
STRING	243273.MG_261.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC71482; AAC71482; MG_261.
GeneID	875381.
KEGG	mge:MG_261.
PATRIC	20010020. VBIMycGen98045_0302.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0587.
KO	K02337.
OMA	IISLFRP.
ProtClustDB	CLSK335230.
Enzyme and pathway databases
BioCyc	MGEN243273:GH2R-287-MONOMER.
Family and domain databases
InterPro	IPR011708. DNA_pol3_alpha. IPR004013. PHP_C. IPR003141. Pol/His_phosphatase_N. IPR004805. PolC_alpha. [Graphical view]
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00594. polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DPO3A_MYCGE

Accession

Primary (citable) accession number: Q49405

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	May 29, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program