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Q49405 (DPO3A_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA polymerase III subunit alpha

EC=2.7.7.7
Gene names
Name:dnaE
Ordered Locus Names:MG261
OrganismMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]
Taxonomic identifier243273 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length874 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase By similarity.

Catalytic activity

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Subunit structure

DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the PolIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the DNA polymerase type-C family. DnaE subfamily.

Ontologies

Keywords
   Biological processDNA replication
   Cellular componentCytoplasm
   Molecular functionDNA-directed DNA polymerase
Nucleotidyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function3'-5' exonuclease activity

Inferred from electronic annotation. Source: InterPro

DNA binding

Inferred from electronic annotation. Source: InterPro

DNA-directed DNA polymerase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 874874DNA polymerase III subunit alpha
PRO_0000103326

Sequences

Sequence LengthMass (Da)Tools
Q49405 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 9B27C96A28A74909

FASTA874100,435
        10         20         30         40         50         60 
MFVNLHTNSY YNFLNSALSP KKLVNLAIND QQKAVAITDP NLFGAVEFFI TCKQNNIKPI 

        70         80         90        100        110        120 
IGLNLTVEYQ KNDVKLLLIA KSNKGFQTLN KIALIQQKLE INSLVDQLTD IAVIICSLTT 

       130        140        150        160        170        180 
WKSTYKDVYQ AKGIEINQTP IAILANAVNC EKTNSDQVVL TVLKQMKQNQ TGKITTFDWD 

       190        200        210        220        230        240 
LKQKLNQISI NENLKVKSEI QPFLDQKTAQ QLFSETELNN LNDLVNRCEL DLEHLKAASL 

       250        260        270        280        290        300 
SLTDNDAAVL ESLCQTNLKQ FLDKNQDLNK KAYQLRLEKE LNVINKLNFA SYFLVVNDLV 

       310        320        330        340        350        360 
NYAFKKDILI GSGRGSAVGS LVAFLLNITK IDPVQHQLIF ERFISTHRQD LPDIDIDIME 

       370        380        390        400        410        420 
NKRAEMINYL FEKYGKENCA QIVTFQRFKT RSAVKEVAKL FNDYGISDMI LGVLPKDQTI 

       430        440        450        460        470        480 
TFTDLKATED SALQLCLQQF GLIVELALAI VDFPRQSSIH ASGIVIASNS LIKTIPLLQL 

       490        500        510        520        530        540 
DNNHFLTQVS MEWLSFFNLN KFDLLGLINL TMISDVITQI KPSNQTVNQF LNTISWTDQN 

       550        560        570        580        590        600 
TFINLVNEDT LGIFQLESFG MKKLLVQIKP KTINQLAIVL ALYRPGAQDN INLFINRLHN 

       610        620        630        640        650        660 
GYDQSDIDPR ILPIVKNTYG VLIFQEQIIN IVKVVANYSL EEADSFRRAI SKKDVKLIQK 

       670        680        690        700        710        720 
NKRNFFERAV QNNFDLKTTT KIFSYIERFA NYGFNLSHAL GYALLSYWTA WLKTNYPVYF 

       730        740        750        760        770        780 
YLWLLNHFQS SKDKQKLIIR TLEKSGIEIY PPLLNKAQPN SVIENKKIYL GLNLIKGIND 

       790        800        810        820        830        840 
RYIQNLQKVQ HLIQTQNNLQ LTDVVSWCLD KTIGDIPLKD LLLLKTMGCF DFFEYTYDFN 

       850        860        870 
DAKDFWIKSD HLLFTRMPLE KKDSNFWIKQ FFTN 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L43967 Genomic DNA. Translation: AAC71482.1.
PIRH64228.
RefSeqNP_072927.1. NC_000908.2.

3D structure databases

ProteinModelPortalQ49405.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243273.MG_261.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC71482; AAC71482; MG_261.
GeneID875381.
KEGGmge:MG_261.
PATRIC20010020. VBIMycGen98045_0302.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0587.
KOK02337.
OMAEYETICK.
OrthoDBEOG6CZQGR.
ProtClustDBCLSK335230.

Enzyme and pathway databases

BioCycMGEN243273:GH2R-287-MONOMER.

Family and domain databases

InterProIPR011708. DNA_pol3_alpha.
IPR004013. PHP_C.
IPR003141. Pol/His_phosphatase_N.
IPR016195. Pol/histidinol_Pase-like.
IPR004805. PolC_alpha.
[Graphical view]
PfamPF07733. DNA_pol3_alpha. 1 hit.
PF02811. PHP. 1 hit.
[Graphical view]
SMARTSM00481. POLIIIAc. 1 hit.
[Graphical view]
SUPFAMSSF89550. SSF89550. 1 hit.
TIGRFAMsTIGR00594. polc. 1 hit.
ProtoNetSearch...

Entry information

Entry nameDPO3A_MYCGE
AccessionPrimary (citable) accession number: Q49405
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Mycoplasma genitalium

Mycoplasma genitalium (strain G-37): entries and gene names