Reviewed, UniProtKB/Swiss-Prot Q493Z9 (SYV_BLOPB)

Names and origin

Protein names

Recommended name:
    Valyl-tRNA synthetase
    EC=6.1.1.9
Alternative name(s):
    Valine--tRNA ligase
      Short name=ValRS

Gene names

Name:	valS
Ordered Locus Names:	BPEN_033

Organism

Blochmannia pennsylvanicus (strain BPEN) [Complete proteome] [HAMAP]

Taxonomic identifier

291272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › ant endosymbionts › Candidatus Blochmannia

Protein attributes

Sequence length	957 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a "posttransfer" editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner By similarity.
Catalytic activity	ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val). HAMAP MF_02004
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site By similarity. The C-terminal coiled-coil domain is crucial for aminoacylation activity By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Domain	Coiled coil
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	valyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP valine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 957	957	Valyl-tRNA synthetase HAMAP MF_02004		PRO_0000224444
Regions
	Coiled coil	889 – 918	30	Potential
	Motif	47 – 57	11	"HIGH" region HAMAP MF_02004
	Motif	558 – 562	5	"KMSKS" region HAMAP MF_02004
Sites
	Binding site	561	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q493Z9-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 80ADDA20B51C1C70	FASTA	957	112,037
	10 20 30 40 50 60 MSNYIVEKIY NPKNIEEPIY KFWEYGDYFS PHGNTSQESY CIMMPPPNIT GQLHLGHAFQ 70 80 90 100 110 120 QTIMDVLIRY QRMQGKNTLW QTGTDHAGIA TQMLVEHKIY NNTGKTRHDY TRDELIKNIW 130 140 150 160 170 180 AWKSQSEQFI TYQMKRLGNS VDWKRQRFTM DTEMSYAVTE AFIRLYRKNL IYRGKRLVNW 190 200 210 220 230 240 DCKLQTAISD LEVINKKTKG SIWYIYYKLD NATISSNSHH LIVATTRPET MLGDTAVAVH 250 260 270 280 290 300 PEDTRYKNYI GQYVIAPITN RRIPIISDKN VDMFKGTGCL KITPAHDFND YIIGKRHGLP 310 320 330 340 350 360 MINIFSLNGK ILKKLEVFNS SGQLTDQLYC KIPQIFHNLD SDNARKKIIS ECNALKLLHN 370 380 390 400 410 420 IEPHDLTIPY SDRTGTIIEP MLTDQWYIRV KHLTQHAIDA VNLNIINFVP KQYKNMYFSW 430 440 450 460 470 480 MNNLQDWCIS RQIWWGHKIP AWYDDNNTIY VGYCEKDIRI KNKLNNNVIL HREKDVLDTW 490 500 510 520 530 540 FSSSLWTFAA LGWPKNTNLL NVFHPTNIII SGFDIIFFWI ARMIMLTMHF IKNDNGSAQI 550 560 570 580 590 600 PFKTVYITGL IRDELGQKMS KSKGNIIDPI DIIDGISIEN LLKKRTKNML QPQLSKHIIN 610 620 630 640 650 660 NTIKQFPNGI KPHGTDALRF TLVALASSGR DIHWDMQRLT GYRNFCNKLW HASRFVLMHT 670 680 690 700 710 720 KNQDCGISIN INEKSFSLAD RWIITKFHQT VQIFHKKLEI YRFDEIANIL HEFIWHQFCD 730 740 750 760 770 780 WYLELTKPIL YHGNALELRG TRYTLITLLE SLLRLTHPII PFITEKIWQE VKTVTGNNGT 790 800 810 820 830 840 TIMLQPFPKY DESVIDMKSV IDIEWIKNAV LAIRTARVNM NISYNIPLQI VFRDTSSEVK 850 860 870 880 890 900 KRITENSKIL CHIAQLKSIH FISKGTIYPK SMTMPLDSSE LLIRIPDTFN KENEINRLKK 910 920 930 940 950 ESELINRKIE TIQKLLDDNN FINQAPKSVI KDKQALLNYY ELIQNKLIDQ CAIMKKL

References

[1]	"Genome sequence of Blochmannia pennsylvanicus indicates parallel evolutionary trends among bacterial mutualists of insects." Degnan P.H., Lazarus A.B., Wernegreen J.J. Genome Res. 15:1023-1033(2005) [PubMed: 16077009] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000016 Genomic DNA. Translation: AAZ40682.1.
RefSeq	YP_277554.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3562918.
GenomeReviews	Gene locus BPEN_033 in contig CP000016_GR.
KEGG	bpn:BPEN_033.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q493Z9.
OMA	Q493Z9. WPDETPE.
Enzyme and pathway databases
BioCyc	CBLO291272:BPEN_033-MON.
Family and domain databases
HAMAP	MF_02004. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR014729. Rossmann-like_a/b/a_fold. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR002303. Val-tRNA_synth_Ia. IPR019754. Val-tRNA_synth_Ia_N. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11946:SF5. tRNA-synt_val. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. [Graphical view]
PRINTS	PR00986. TRNASYNTHVAL.
TIGRFAMs	TIGR00422. valS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYV_BLOPB

Accession

Primary (citable) accession number: Q493Z9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	September 13, 2005
Last modified:	June 16, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)