Reviewed, UniProtKB/Swiss-Prot Q493T3 (PNP_BLOPB)

Names and origin

Protein names

Recommended name:
    Polyribonucleotide nucleotidyltransferase
    EC=2.7.7.8
Alternative name(s):
    Polynucleotide phosphorylase
      Short name=PNPase

Gene names

Name:	pnp
Ordered Locus Names:	BPEN_112

Organism

Blochmannia pennsylvanicus (strain BPEN) [Complete proteome] [HAMAP]

Taxonomic identifier

291272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › ant endosymbionts › Candidatus Blochmannia

Protein attributes

Sequence length	699 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity.
Catalytic activity	RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate. HAMAP MF_01595
Subunit structure	Homotrimer. Organized into a structure (processome or RNA degradosome) containing a number of RNA-processing enzymes By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the polyribonucleotide nucleotidyltransferase family. Contains 1 KH domain. Contains 1 S1 motif domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	RNA-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro mRNA catabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5'-exoribonuclease activity Inferred from electronic annotation. Source: InterPro RNA binding Inferred from electronic annotation. Source: UniProtKB-KW polyribonucleotide nucleotidyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 699	699	Polyribonucleotide nucleotidyltransferase HAMAP MF_01595		PRO_0000329534
Regions
	Domain	555 – 614	60	KH
	Domain	624 – 692	69	S1 motif

Sequences

	Sequence		Length	Mass (Da)
	Q493T3-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 4914F2EB0F17C4B9	FASTA	699	77,476
	10 20 30 40 50 60 MLDPIIHKFK YGQHTVILDI GTIARQANAA VMVSMSDTTV LVTVVSSNQV RLEQDFFPLV 70 80 90 100 110 120 VNYQERTYAA GKFPGGFFRR EGRPSENETL TSRLIDRPIR PLFPKGFIND VQVVATVVSV 130 140 150 160 170 180 NPQVNPDIVA IIGVSAALSL SDIPFFGPIG AARVGYIKNQ YVLNPTTTEL SNSKLDLVIS 190 200 210 220 230 240 GTESNILMVE SESCLLSEKE ILDAIIFGHE QQQIVIHNIN KLVKKVGKTK YSWNEKEVNI 250 260 270 280 290 300 SLKTYLSELY ESRAQDIYCF FDKKERHAQV DSIKKDIVKT VLNSYQHAAV DEKEIMYLLN 310 320 330 340 350 360 YLEAQSIRYR ILTNKLRIDG RSQDTIRKID IKTGILPRTH GSALFTRGDT QALVAVTLGT 370 380 390 400 410 420 ERDAQNIDGL TGIRVDRFLL HYNFPPYCVG EIGVIGAPKR REIGHGRLAK RGMLAVMPNA 430 440 450 460 470 480 NEFPYTIRVV SEITESNGSS SMASICGTSL ALMDAGVPIK AAVAGVAMGL IKEGNDFVVL 490 500 510 520 530 540 SDIVSDEDHI GDMDFKVSGS RQGITALQMD IKTDGITYDI INIALKKAKN ARLQILDVME 550 560 570 580 590 600 QIIKFPNQEI SKFAPRIYSI KVNPDKIKDV IGKGGSVIRS LTEETNTIID IEDNGIIKIV 610 620 630 640 650 660 ALDYDKAKQA IRRINDITAN VEIGAVYTGK VSHIVEFGAF VTILSGKEGL VHISQISERR 670 680 690 INKVTDYLKL GQKVLVKVLE IDRQGRIRLS MKGVHQVSA

References

[1]	"Genome sequence of Blochmannia pennsylvanicus indicates parallel evolutionary trends among bacterial mutualists of insects." Degnan P.H., Lazarus A.B., Wernegreen J.J. Genome Res. 15:1023-1033(2005) [PubMed: 16077009] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000016 Genomic DNA. Translation: AAZ40752.1.
RefSeq	YP_277625.1.
3D structure databases
SMR	Q493T3. Positions 619-692.
ModBase	Search...
Genome annotation databases
GeneID	3563170.
GenomeReviews	Gene locus BPEN_112 in contig CP000016_GR.
KEGG	bpn:BPEN_112.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q493T3.
OMA	Q493T3. GHGNLAK.
Enzyme and pathway databases
BioCyc	CBLO291272:BPEN_112-MON.
Family and domain databases
HAMAP	MF_01595. [Tree]
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR004087. KH. IPR004088. KH_type_1. IPR018111. KH_type_1_subgr. IPR012340. NA-bd_OB-fold. IPR012162. PNPase. IPR015848. PNPase_PH_RNA-bd_bac/org-type. IPR003029. Rbsml_prot_S1_RNA-bd_dom. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:1.10.10.400. PNPase_PH_RNA-bd_bac/org-type. 1 hit.
PANTHER	PTHR11252. PNPase. 1 hit.
Pfam	PF00013. KH_1. 1 hit. PF03726. PNPase. 1 hit. PF01138. RNase_PH. 2 hits. PF03725. RNase_PH_C. 2 hits. PF00575. S1. 1 hit. [Graphical view]
PIRSF	PIRSF005499. PNPase. 1 hit.
SMART	SM00322. KH. 1 hit. [Graphical view]
TIGRFAMs	TIGR03591. Polynuc_phos. 1 hit.
PROSITE	PS50084. KH_TYPE_1. 1 hit. PS50126. S1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PNP_BLOPB

Accession

Primary (citable) accession number: Q493T3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	September 13, 2005
Last modified:	June 16, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)