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Q493S3

- SYI_BLOPB

UniProt

Q493S3 - SYI_BLOPB

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Protein

Isoleucine--tRNA ligase

Gene
ileS, BPEN_122
Organism
Blochmannia pennsylvanicus (strain BPEN)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei564 – 5641Aminoacyl-adenylate By similarity
Binding sitei608 – 6081ATP By similarity
Metal bindingi905 – 9051Zinc By similarity
Metal bindingi908 – 9081Zinc By similarity
Metal bindingi925 – 9251Zinc By similarity
Metal bindingi928 – 9281Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:BPEN_122
OrganismiBlochmannia pennsylvanicus (strain BPEN)
Taxonomic identifieri291272 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeant endosymbiontsCandidatus Blochmannia
ProteomesiUP000007794: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 942942Isoleucine--tRNA ligaseUniRule annotationPRO_0000098356Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi291272.BPEN_122.

Structurei

3D structure databases

ProteinModelPortaliQ493S3.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotationAdd
BLAST
Motifi605 – 6095"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q493S3-1 [UniParc]FASTAAdd to Basket

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MINYKNTLNL PYTLFPMRAN LPVCEPTILE RWYKDNLYEA IRHKKSKKKL    50
FILHDGPPYV NGSIHLGHAV NKVLKDIILK YKTLTGYNAP YIPGWDCHGL 100
PIELQVERLI GQLGVNVDPN EFRSICRNYV LDQIEIQKKD FVRLGVLGDW 150
SRPYLTMDFK TEANIIRTLS KVISNGYFYK GTKPVHWCFQ CCSVLAESEV 200
EYNNHCSPAI DVAFFAVDNI RISKIFNIDL CQRVIELVIW TTTPWTLPSN 250
QAISVHPDYS YQLIAINNSK YIIIAANLVN TVMQRIQCFS WRILGETSGN 300
SLESLKFRHP FMSFDVPVVL SGHVALDSGT GVVHIAPNHG VDDYIIARKY 350
NFKIVNIINE TGHYVSNVHP MLDGVQVFQS NEIIIKLLNQ SGSLLHVNHN 400
YKHNYPYCWR HAVPVIFKAT AQWFLSMDKY NLRNKLLQTI HQVDWIPNKG 450
EYSIETMIVN RPDWCLSRQR VWGVPIPLFI HKKTSILHPR TIALMEVIAQ 500
HVEQNGIQAW WDLKTEDIID NEEADQYEKV LDTLDVWFDS GSTHYSVIPE 550
RLEYRKQSPD LYLEGSDQYR GWFMSSLIIS TVITGQAPYR EVLTHGFTVD 600
AQGRKMSKSL GNVISPQEII KDFGADILRL WIASSDYSKE MAISNAILKH 650
TIDVYRRIRN TARFCLANIS DFDPEKDSVH PENMIELDCW AIDRALSVQL 700
EVISDYNKYN FHNVIQRIMQ FCSVEMGSFY LDVIKDRQYT TKKNSKERRS 750
CQTALYHIIE SMVRWIAPVL SFTADEIWRH IPGNRSKYVF TEEWYNGLFS 800
ISSKQIMNSH DWNTFLNVRS EVNKIVEQTR LNGIIGGSLD ASVVLYATPE 850
LANTLRILGN ELSFGLITSS AIVSDYCNGF NVTQYTQEGI PGLKIFLEKA 900
KGKKCLRCWH YRLDIGQYKN YSNICARCVN NIVGPGEKRR FF 942
Length:942
Mass (Da):108,694
Last modified:September 13, 2005 - v1
Checksum:iD9724149300EDD20
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000016 Genomic DNA. Translation: AAZ40762.1.
RefSeqiYP_277635.1. NC_007292.1.

Genome annotation databases

EnsemblBacteriaiAAZ40762; AAZ40762; BPEN_122.
GeneIDi3563125.
KEGGibpn:BPEN_122.
PATRICi31965135. VBICanBlo110049_0117.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000016 Genomic DNA. Translation: AAZ40762.1 .
RefSeqi YP_277635.1. NC_007292.1.

3D structure databases

ProteinModelPortali Q493S3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 291272.BPEN_122.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAZ40762 ; AAZ40762 ; BPEN_122 .
GeneIDi 3563125.
KEGGi bpn:BPEN_122.
PATRICi 31965135. VBICanBlo110049_0117.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of Blochmannia pennsylvanicus indicates parallel evolutionary trends among bacterial mutualists of insects."
    Degnan P.H., Lazarus A.B., Wernegreen J.J.
    Genome Res. 15:1023-1033(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BPEN.

Entry informationi

Entry nameiSYI_BLOPB
AccessioniPrimary (citable) accession number: Q493S3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: September 13, 2005
Last modified: May 14, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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