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Q493S3

- SYI_BLOPB

UniProt

Q493S3 - SYI_BLOPB

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Blochmannia pennsylvanicus (strain BPEN)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 68 (01 Oct 2014)
      Sequence version 1 (13 Sep 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei564 – 5641Aminoacyl-adenylateUniRule annotation
    Binding sitei608 – 6081ATPUniRule annotation
    Metal bindingi905 – 9051ZincUniRule annotation
    Metal bindingi908 – 9081ZincUniRule annotation
    Metal bindingi925 – 9251ZincUniRule annotation
    Metal bindingi928 – 9281ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:BPEN_122
    OrganismiBlochmannia pennsylvanicus (strain BPEN)
    Taxonomic identifieri291272 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeant endosymbiontsCandidatus Blochmannia
    ProteomesiUP000007794: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 942942Isoleucine--tRNA ligasePRO_0000098356Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi291272.BPEN_122.

    Structurei

    3D structure databases

    ProteinModelPortaliQ493S3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi605 – 6095"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q493S3-1 [UniParc]FASTAAdd to Basket

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    MINYKNTLNL PYTLFPMRAN LPVCEPTILE RWYKDNLYEA IRHKKSKKKL    50
    FILHDGPPYV NGSIHLGHAV NKVLKDIILK YKTLTGYNAP YIPGWDCHGL 100
    PIELQVERLI GQLGVNVDPN EFRSICRNYV LDQIEIQKKD FVRLGVLGDW 150
    SRPYLTMDFK TEANIIRTLS KVISNGYFYK GTKPVHWCFQ CCSVLAESEV 200
    EYNNHCSPAI DVAFFAVDNI RISKIFNIDL CQRVIELVIW TTTPWTLPSN 250
    QAISVHPDYS YQLIAINNSK YIIIAANLVN TVMQRIQCFS WRILGETSGN 300
    SLESLKFRHP FMSFDVPVVL SGHVALDSGT GVVHIAPNHG VDDYIIARKY 350
    NFKIVNIINE TGHYVSNVHP MLDGVQVFQS NEIIIKLLNQ SGSLLHVNHN 400
    YKHNYPYCWR HAVPVIFKAT AQWFLSMDKY NLRNKLLQTI HQVDWIPNKG 450
    EYSIETMIVN RPDWCLSRQR VWGVPIPLFI HKKTSILHPR TIALMEVIAQ 500
    HVEQNGIQAW WDLKTEDIID NEEADQYEKV LDTLDVWFDS GSTHYSVIPE 550
    RLEYRKQSPD LYLEGSDQYR GWFMSSLIIS TVITGQAPYR EVLTHGFTVD 600
    AQGRKMSKSL GNVISPQEII KDFGADILRL WIASSDYSKE MAISNAILKH 650
    TIDVYRRIRN TARFCLANIS DFDPEKDSVH PENMIELDCW AIDRALSVQL 700
    EVISDYNKYN FHNVIQRIMQ FCSVEMGSFY LDVIKDRQYT TKKNSKERRS 750
    CQTALYHIIE SMVRWIAPVL SFTADEIWRH IPGNRSKYVF TEEWYNGLFS 800
    ISSKQIMNSH DWNTFLNVRS EVNKIVEQTR LNGIIGGSLD ASVVLYATPE 850
    LANTLRILGN ELSFGLITSS AIVSDYCNGF NVTQYTQEGI PGLKIFLEKA 900
    KGKKCLRCWH YRLDIGQYKN YSNICARCVN NIVGPGEKRR FF 942
    Length:942
    Mass (Da):108,694
    Last modified:September 13, 2005 - v1
    Checksum:iD9724149300EDD20
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000016 Genomic DNA. Translation: AAZ40762.1.
    RefSeqiYP_277635.1. NC_007292.1.

    Genome annotation databases

    EnsemblBacteriaiAAZ40762; AAZ40762; BPEN_122.
    GeneIDi3563125.
    KEGGibpn:BPEN_122.
    PATRICi31965135. VBICanBlo110049_0117.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000016 Genomic DNA. Translation: AAZ40762.1 .
    RefSeqi YP_277635.1. NC_007292.1.

    3D structure databases

    ProteinModelPortali Q493S3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 291272.BPEN_122.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAZ40762 ; AAZ40762 ; BPEN_122 .
    GeneIDi 3563125.
    KEGGi bpn:BPEN_122.
    PATRICi 31965135. VBICanBlo110049_0117.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of Blochmannia pennsylvanicus indicates parallel evolutionary trends among bacterial mutualists of insects."
      Degnan P.H., Lazarus A.B., Wernegreen J.J.
      Genome Res. 15:1023-1033(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: BPEN.

    Entry informationi

    Entry nameiSYI_BLOPB
    AccessioniPrimary (citable) accession number: Q493S3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: September 13, 2005
    Last modified: October 1, 2014
    This is version 68 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3