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Q493S3

- SYI_BLOPB

UniProt

Q493S3 - SYI_BLOPB

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Blochmannia pennsylvanicus (strain BPEN)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei564 – 5641Aminoacyl-adenylateUniRule annotation
Binding sitei608 – 6081ATPUniRule annotation
Metal bindingi905 – 9051ZincUniRule annotation
Metal bindingi908 – 9081ZincUniRule annotation
Metal bindingi925 – 9251ZincUniRule annotation
Metal bindingi928 – 9281ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:BPEN_122
OrganismiBlochmannia pennsylvanicus (strain BPEN)
Taxonomic identifieri291272 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeant endosymbiontsCandidatus Blochmannia
ProteomesiUP000007794: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 942942Isoleucine--tRNA ligasePRO_0000098356Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi291272.BPEN_122.

Structurei

3D structure databases

ProteinModelPortaliQ493S3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi605 – 6095"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q493S3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MINYKNTLNL PYTLFPMRAN LPVCEPTILE RWYKDNLYEA IRHKKSKKKL
60 70 80 90 100
FILHDGPPYV NGSIHLGHAV NKVLKDIILK YKTLTGYNAP YIPGWDCHGL
110 120 130 140 150
PIELQVERLI GQLGVNVDPN EFRSICRNYV LDQIEIQKKD FVRLGVLGDW
160 170 180 190 200
SRPYLTMDFK TEANIIRTLS KVISNGYFYK GTKPVHWCFQ CCSVLAESEV
210 220 230 240 250
EYNNHCSPAI DVAFFAVDNI RISKIFNIDL CQRVIELVIW TTTPWTLPSN
260 270 280 290 300
QAISVHPDYS YQLIAINNSK YIIIAANLVN TVMQRIQCFS WRILGETSGN
310 320 330 340 350
SLESLKFRHP FMSFDVPVVL SGHVALDSGT GVVHIAPNHG VDDYIIARKY
360 370 380 390 400
NFKIVNIINE TGHYVSNVHP MLDGVQVFQS NEIIIKLLNQ SGSLLHVNHN
410 420 430 440 450
YKHNYPYCWR HAVPVIFKAT AQWFLSMDKY NLRNKLLQTI HQVDWIPNKG
460 470 480 490 500
EYSIETMIVN RPDWCLSRQR VWGVPIPLFI HKKTSILHPR TIALMEVIAQ
510 520 530 540 550
HVEQNGIQAW WDLKTEDIID NEEADQYEKV LDTLDVWFDS GSTHYSVIPE
560 570 580 590 600
RLEYRKQSPD LYLEGSDQYR GWFMSSLIIS TVITGQAPYR EVLTHGFTVD
610 620 630 640 650
AQGRKMSKSL GNVISPQEII KDFGADILRL WIASSDYSKE MAISNAILKH
660 670 680 690 700
TIDVYRRIRN TARFCLANIS DFDPEKDSVH PENMIELDCW AIDRALSVQL
710 720 730 740 750
EVISDYNKYN FHNVIQRIMQ FCSVEMGSFY LDVIKDRQYT TKKNSKERRS
760 770 780 790 800
CQTALYHIIE SMVRWIAPVL SFTADEIWRH IPGNRSKYVF TEEWYNGLFS
810 820 830 840 850
ISSKQIMNSH DWNTFLNVRS EVNKIVEQTR LNGIIGGSLD ASVVLYATPE
860 870 880 890 900
LANTLRILGN ELSFGLITSS AIVSDYCNGF NVTQYTQEGI PGLKIFLEKA
910 920 930 940
KGKKCLRCWH YRLDIGQYKN YSNICARCVN NIVGPGEKRR FF
Length:942
Mass (Da):108,694
Last modified:September 13, 2005 - v1
Checksum:iD9724149300EDD20
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000016 Genomic DNA. Translation: AAZ40762.1.
RefSeqiYP_277635.1. NC_007292.1.

Genome annotation databases

EnsemblBacteriaiAAZ40762; AAZ40762; BPEN_122.
GeneIDi3563125.
KEGGibpn:BPEN_122.
PATRICi31965135. VBICanBlo110049_0117.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000016 Genomic DNA. Translation: AAZ40762.1 .
RefSeqi YP_277635.1. NC_007292.1.

3D structure databases

ProteinModelPortali Q493S3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 291272.BPEN_122.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAZ40762 ; AAZ40762 ; BPEN_122 .
GeneIDi 3563125.
KEGGi bpn:BPEN_122.
PATRICi 31965135. VBICanBlo110049_0117.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of Blochmannia pennsylvanicus indicates parallel evolutionary trends among bacterial mutualists of insects."
    Degnan P.H., Lazarus A.B., Wernegreen J.J.
    Genome Res. 15:1023-1033(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BPEN.

Entry informationi

Entry nameiSYI_BLOPB
AccessioniPrimary (citable) accession number: Q493S3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: September 13, 2005
Last modified: November 26, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3