Reviewed, UniProtKB/Swiss-Prot Q493S2 (LSPA_BLOPB)

Names and origin

Protein names

Recommended name:
    Lipoprotein signal peptidase
    EC=3.4.23.36
Alternative name(s):
    Prolipoprotein signal peptidase
    Signal peptidase II
      Short name=SPase II

Gene names

Name:	lspA
Ordered Locus Names:	BPEN_123

Organism

Blochmannia pennsylvanicus (strain BPEN) [Complete proteome] [HAMAP]

Taxonomic identifier

291272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › ant endosymbionts › Candidatus Blochmannia

Protein attributes

Sequence length	165 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This protein specifically catalyzes the removal of signal peptides from prolipoproteins By similarity.
Catalytic activity	Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains. HAMAP MF_00161
Pathway	Protein modification; lipoprotein biosynthesis (signal peptide cleavage). HAMAP MF_00161
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the peptidase A8 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Molecular function	Aspartyl protease Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 165	165	Lipoprotein signal peptidase HAMAP MF_00161		PRO_1000076917
Regions
	Transmembrane	10 – 30	21	Potential
	Transmembrane	42 – 62	21	Potential
	Transmembrane	71 – 91	21	Potential
	Transmembrane	105 – 125	21	Potential
	Transmembrane	133 – 153	21	Potential
Sites
	Active site	114	1	By similarity
	Active site	141	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q493S2-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 0738296C201A6AAA	FASTA	165	19,343
	10 20 30 40 50 60 MNNKYNYKNL KWLWLSILIM LLDIGTKYWV KTHFWIGEVL SVLPGINCYY VCNPGLAFGL 70 80 90 100 110 120 FTNASLYYRW LFVWIITLVI VAFIIALYKL IERPKCYSIS YSMVIGGALG NLLDRILYGA 130 140 150 160 VVDFIDVHIK NWHWPTFNVA DIAICIGITI VTIRFYYDFI KNNLY

References

[1]	"Genome sequence of Blochmannia pennsylvanicus indicates parallel evolutionary trends among bacterial mutualists of insects." Degnan P.H., Lazarus A.B., Wernegreen J.J. Genome Res. 15:1023-1033(2005) [PubMed: 16077009] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000016 Genomic DNA. Translation: AAZ40763.1.
RefSeq	YP_277636.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q493S2.
Protein family/group databases
MEROPS	A08.001.
Genome annotation databases
GeneID	3563126.
GenomeReviews	Gene locus BPEN_123 in contig CP000016_GR.
KEGG	bpn:BPEN_123.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q493S2.
OMA	IAVDHYL.
Enzyme and pathway databases
BioCyc	CBLO291272:BPEN_123-MON.
Family and domain databases
HAMAP	MF_00161. [Tree]
InterPro	IPR001872. Peptidase_A8. [Graphical view]
Pfam	PF01252. Peptidase_A8. 1 hit. [Graphical view]
PRINTS	PR00781. LIPOSIGPTASE.
ProDom	PD004304. Peptidase_A8. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00077. lspA. 1 hit.
PROSITE	PS00855. SPASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LSPA_BLOPB

Accession

Primary (citable) accession number: Q493S2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	September 13, 2005
Last modified:	November 3, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)