Reviewed, UniProtKB/Swiss-Prot Q493R1 (LEU3_BLOPB)

Names and origin

Protein names

Recommended name:
    3-isopropylmalate dehydrogenase
    EC=1.1.1.85
Alternative name(s):
    Beta-IPM dehydrogenase
      Short name=IMDH
    3-IPM-DH

Gene names

Name:	leuB
Ordered Locus Names:	BPEN_136

Organism

Blochmannia pennsylvanicus (strain BPEN) [Complete proteome] [HAMAP]

Taxonomic identifier

291272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › ant endosymbionts › Candidatus Blochmannia

Protein attributes

Sequence length	366 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate. HAMAP MF_01033
Catalytic activity	(2R,3S)-3-isopropylmalate + NAD(+) = 4-methyl-2-oxopentanoate + CO(2) + NADH. HAMAP MF_01033
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4. HAMAP MF_01033
Subunit structure	Homodimer By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Leucine biosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium Manganese Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	leucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	3-isopropylmalate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 366	366	3-isopropylmalate dehydrogenase HAMAP MF_01033		PRO_0000083648
Regions
	Nucleotide binding	78 – 91	14	NAD By similarity
	Nucleotide binding	285 – 297	13	NAD By similarity
Sites
	Metal binding	227	1	Magnesium or manganese By similarity
	Metal binding	251	1	Magnesium or manganese By similarity
	Metal binding	255	1	Magnesium or manganese By similarity
	Binding site	99	1	Substrate By similarity
	Binding site	109	1	Substrate By similarity
	Binding site	138	1	Substrate By similarity
	Binding site	227	1	Substrate By similarity
	Site	145	1	Important for catalysis By similarity
	Site	195	1	Important for catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q493R1-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: F93A9E60886990C9	FASTA	366	40,664
	10 20 30 40 50 60 MNNNYHIAVL PGDGIGPETT RQVYKILNVI KKQFQINIVT TEHKIGGDAI NSEGTPFPKS 70 80 90 100 110 120 TLKYCEQSNA ILFGAVGGPQ WTHLKGSESP EQGALLALRK HFNLFANLRP IYLADELKEL 130 140 150 160 170 180 SPLNINMIPN GIDIIFVREL TGGIYFGQPK GRSGTGLNEY AFDTAVYHRF EIERIANIAF 190 200 210 220 230 240 KLAQKRRKRV SSIDKANVLH TSMLWREVVS HLAKNYPDVE LEHLYIDNAS MQLINNPSKF 250 260 270 280 290 300 DIILCPNLFG DILSDECAMI SGSIGMLPSA SINEHNFGLY EPAGGSAPDI AEKNIANPIA 310 320 330 340 350 360 HILSIALLFR YSLKLDHIAI KIEKAVSQAL MLGYRTIDIA KKHEKSISTN EMGDIIAALI KNEREK

References

[1]	"Genome sequence of Blochmannia pennsylvanicus indicates parallel evolutionary trends among bacterial mutualists of insects." Degnan P.H., Lazarus A.B., Wernegreen J.J. Genome Res. 15:1023-1033(2005) [PubMed: 16077009] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000016 Genomic DNA. Translation: AAZ40776.1.
RefSeq	YP_277649.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3563104.
GenomeReviews	Gene locus BPEN_136 in contig CP000016_GR.
KEGG	bpn:BPEN_136.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q493R1.
Enzyme and pathway databases
BioCyc	CBLO291272:BPEN_136-MON.
Family and domain databases
HAMAP	MF_01033. [Tree]
InterPro	IPR004429. 3-isopropylmalate_DHase. IPR001804. IsoCit_IM_DHase. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11835. IDH_IMDH_dimeric. 1 hit. PTHR11835:SF13. IPMDH. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR00169. leuB. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEU3_BLOPB

Accession

Primary (citable) accession number: Q493R1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	September 13, 2005
Last modified:	November 4, 2008
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)