Reviewed, UniProtKB/Swiss-Prot Q493Q3 (MURD_BLOPB)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoylalanine--D-glutamate ligase
    EC=6.3.2.9
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
    D-glutamic acid-adding enzyme

Gene names

Name:	murD
Ordered Locus Names:	BPEN_144

Organism

Blochmannia pennsylvanicus (strain BPEN) [Complete proteome] [HAMAP]

Taxonomic identifier

291272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › ant endosymbionts › Candidatus Blochmannia

Protein attributes

Sequence length	440 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 440	440	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_0000257167
Regions
	Nucleotide binding	112 – 118	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q493Q3-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: A45C39BDCEFCDA1C	FASTA	440	49,313
	10 20 30 40 50 60 MRNYRGSKVV IIGLGITGLS CVNFFLDRGV IPKVIDTRIY PPGIKKIPHV VQCYLGAFND 70 80 90 100 110 120 IWLLSATLIV VSPGVRLDHP VLIEALKLGI EIIGDIELFT REATAPIIAI TGSNGKSTVT 130 140 150 160 170 180 QLVSRMARIA GWHVGVAGNI GVPVLSLLNK SYQLYILEIS SFQLDTTYSL RAIAAAILNV 190 200 210 220 230 240 SEDHMDHYPG GLKQYWFSKQ RIYKNAKICV MNALDFLTIP IYHEYDYCIS FGENEDSADY 250 260 270 280 290 300 YLKYYKGHTW IVAYNEYVLN CSEMRINNRI NYINALSALA LSDIIKIPRS VSLKVLCQFS 310 320 330 340 350 360 GLAHRCQLIY KNHGVSWIND SKATNVSATK EAINNLKLCG TLHLILGGDG KLADFSSLKH 370 380 390 400 410 420 LIKQHEIHLY CFGKDGLLLT TLGFSDVILT NTMIQAMRII NRRIKAKDIV LLSPACSSLD 430 440 QFKSFEMRGL IFTCFAREFR

References

[1]	"Genome sequence of Blochmannia pennsylvanicus indicates parallel evolutionary trends among bacterial mutualists of insects." Degnan P.H., Lazarus A.B., Wernegreen J.J. Genome Res. 15:1023-1033(2005) [PubMed: 16077009] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000016 Genomic DNA. Translation: AAZ40784.1.
RefSeq	YP_277657.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3562866.
GenomeReviews	Gene locus BPEN_144 in contig CP000016_GR.
KEGG	bpn:BPEN_144.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q493Q3.
OMA	Q493Q3. LWVNDTK.
Enzyme and pathway databases
BioCyc	CBLO291272:BPEN_144-MON.
Family and domain databases
HAMAP	MF_00639. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR016040. NAD(P)-bd_dom. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01087. murD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_BLOPB

Accession

Primary (citable) accession number: Q493Q3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	September 13, 2005
Last modified:	June 16, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)