Reviewed, UniProtKB/Swiss-Prot Q493N6 (PYRG_BLOPB)

Names and origin

Protein names

Recommended name:
    CTP synthase
    EC=6.3.4.2
Alternative name(s):
    UTP--ammonia ligase
    CTP synthetase

Gene names

Name:	pyrG
Ordered Locus Names:	BPEN_161

Organism

Blochmannia pennsylvanicus (strain BPEN) [Complete proteome] [HAMAP]

Taxonomic identifier

291272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › ant endosymbionts › Candidatus Blochmannia

Protein attributes

Sequence length	548 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen By similarity.
Catalytic activity	ATP + UTP + NH3 = ADP + phosphate + CTP. HAMAP MF_01227
Enzyme regulation	Allosterically activated by GTP, when glutamine is the substrate. Inhibited by CTP By similarity.
Pathway	Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2. HAMAP MF_01227
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the CTP synthase family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW CTP synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 548	548	CTP synthase HAMAP MF_01227		PRO_0000266071
Regions
	Domain	291 – 543	253	Glutamine amidotransferase type-1
	Region	1 – 253	253	Aminator domain HAMAP MF_01227
Sites
	Active site	380	1	Nucleophile By similarity
	Active site	516	1	By similarity
	Active site	518	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q493N6-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 57D35AFC0F2C9037	FASTA	548	61,301
	10 20 30 40 50 60 MRVNYIFVTG GVVSSLGKGI ATASLAAVLE ARGLSVTIIK LDPYINMDPG TISPVQHGEV 70 80 90 100 110 120 FITEDGAETD LDLGHYERFI RTKMRHHNNF TAGKIYADVL RKERRGDYLG ATIQIIPHVT 130 140 150 160 170 180 DTIKKWLIAG AFGHDVLLVE IGGTVGDIES LPFLEAIRQM VMEVNREQTL YIHLTLVPFI 190 200 210 220 230 240 AVSGELKTKP TQHSVKELLS IGIQPDILIC RSDRVISNSE RKKISLFCNV PKQAIIALQD 250 260 270 280 290 300 VDSIYKIPAL LKDQGLDNYI CKRFNLNCPE ANLSDWEEVI YYQEHPIGEV TVGMVGKYIE 310 320 330 340 350 360 LVDAYKSVTE ALKHAGIKNR FIVNIRLINS QDVEKLGIEK TLKGLDAILV PGGFGYRGVE 370 380 390 400 410 420 GKILSAQYAR ENNIPYFGIC LGMQVALIEF ARHVAGMPEA NSTEFVTNCK CPVIALITEC 430 440 450 460 470 480 KDENGIFITH NDNTNLGGTM RLGNQACYLI KGSLTHQIYG KSTILERHRH RYEVNNMLLK 490 500 510 520 530 540 HITHAGLSCV GFSKKNNLVE VIEYPNHPWF IGSQFHPEFN STPREGHPLF IGFIKAAIEY QHRHNKLI

References

[1]	"Genome sequence of Blochmannia pennsylvanicus indicates parallel evolutionary trends among bacterial mutualists of insects." Degnan P.H., Lazarus A.B., Wernegreen J.J. Genome Res. 15:1023-1033(2005) [PubMed: 16077009] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000016 Genomic DNA. Translation: AAZ40801.1.
RefSeq	YP_277674.1.
3D structure databases
SMR	Q493N6. Positions 1-546.
ModBase	Search...
Genome annotation databases
GeneID	3563096.
GenomeReviews	Gene locus BPEN_161 in contig CP000016_GR.
KEGG	bpn:BPEN_161.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q493N6.
OMA	Q493N6. EFNNAYR.
Enzyme and pathway databases
BioCyc	CBLO291272:BPEN_161-MON.
Family and domain databases
HAMAP	MF_01227. [Tree]
InterPro	IPR004468. CTP_synthase. IPR017456. CTP_synthase_N. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11550. PyrG_synth. 1 hit.
Pfam	PF06418. CTP_synth_N. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00337. PyrG. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRG_BLOPB

Accession

Primary (citable) accession number: Q493N6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	September 13, 2005
Last modified:	June 16, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)