ID   CYSI_BLOPB              Reviewed;         575 AA.
AC   Q493N3;
DT   26-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   16-JUN-2009, entry version 24.
DE   RecName: Full=Sulfite reductase [NADPH] hemoprotein beta-component;
DE            Short=SIR-HP;
DE            Short=SIRHP;
DE            EC=1.8.1.2;
GN   Name=cysI; OrderedLocusNames=BPEN_164;
OS   Blochmannia pennsylvanicus (strain BPEN).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; ant endosymbionts; Candidatus Blochmannia.
OX   NCBI_TaxID=291272;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16077009; DOI=10.1101/gr.3771305;
RA   Degnan P.H., Lazarus A.B., Wernegreen J.J.;
RT   "Genome sequence of Blochmannia pennsylvanicus indicates parallel
RT   evolutionary trends among bacterial mutualists of insects.";
RL   Genome Res. 15:1023-1033(2005).
CC   -!- FUNCTION: This enzyme catalyzes the 6-electron reduction of
CC       sulfite to sulfide. This is one of several activities required for
CC       the biosynthesis of L-cysteine from sulfate (By similarity).
CC   -!- CATALYTIC ACTIVITY: H(2)S + 3 NADP(+) + 3 H(2)O = sulfite + 3
CC       NADPH.
CC   -!- COFACTOR: Binds 1 siroheme per subunit (By similarity).
CC   -!- COFACTOR: Binds 1 4Fe-4S cluster per subunit (By similarity).
CC   -!- SUBUNIT: Alpha(8)-beta(4). The alpha component is a flavoprotein,
CC       the beta component is a hemoprotein (By similarity).
CC   -!- SIMILARITY: Belongs to the nitrite and sulfite reductase 4Fe-4S
CC       domain family.
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DR   EMBL; CP000016; AAZ40804.1; -; Genomic_DNA.
DR   RefSeq; YP_277677.1; -.
DR   GeneID; 3563110; -.
DR   GenomeReviews; CP000016_GR; BPEN_164.
DR   KEGG; bpn:BPEN_164; -.
DR   HOGENOM; Q493N3; -.
DR   OMA; Q493N3; TRQAFQM.
DR   BioCyc; CBLO291272:BPEN_164-MON; -.
DR   GO; GO:0009337; C:sulfite reductase complex (NADPH); IEA:InterPro.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0050661; F:NADP or NADPH binding; IEA:InterPro.
DR   GO; GO:0004783; F:sulfite reductase (NADPH) activity; IEA:HAMAP.
DR   GO; GO:0019344; P:cysteine biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0000103; P:sulfate assimilation; IEA:HAMAP.
DR   HAMAP; MF_01540; -; 1.
DR   InterPro; IPR011786; CysI.
DR   InterPro; IPR006066; Nir_Si_BS.
DR   InterPro; IPR006067; Nir_Sir_4Fe4S.
DR   InterPro; IPR005117; NiRdtase/SiRdtase_haem-b_fer.
DR   Pfam; PF01077; NIR_SIR; 1.
DR   Pfam; PF03460; NIR_SIR_ferr; 2.
DR   PRINTS; PR00397; SIROHAEM.
DR   TIGRFAMs; TIGR02041; CysI; 1.
DR   PROSITE; PS00365; NIR_SIR; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; Amino-acid biosynthesis; Complete proteome;
KW   Cysteine biosynthesis; Heme; Iron; Iron-sulfur; Metal-binding; NADP;
KW   Oxidoreductase.
FT   CHAIN         1    575       Sulfite reductase [NADPH] hemoprotein
FT                                beta-component.
FT                                /FTId=PRO_0000292959.
FT   METAL       439    439       Iron-sulfur (4Fe-4S) (By similarity).
FT   METAL       445    445       Iron-sulfur (4Fe-4S) (By similarity).
FT   METAL       484    484       Iron-sulfur (4Fe-4S) (By similarity).
FT   METAL       488    488       Iron (siroheme axial ligand) (By
FT                                similarity).
FT   METAL       488    488       Iron-sulfur (4Fe-4S) (By similarity).
SQ   SEQUENCE   575 AA;  65260 MW;  CAF03E18C47DDE31 CRC64;
     MNRKYDNNND KNIPLLSDNE RIKKESNFLR GTIAQNLDNN LTGGFNTEDA QLIRFHGMYQ
     QDDRDVRVER ANQKLEPLIN MMLRCRLPGG VIMPQQWLAI DDFSEKYTLY GTIRLTTRQT
     FQLHGLLKPN LKNVHRLLNK LGLDSIATAG DVNRNVICTA NPMESTLHYQ VWELAKSISS
     YLLPKSNAYA EIWLDSKKTE STDSEPILSA TYLPRKFKIA IAIPPINDVD VHANDLSFIA
     IKSENTDQII GFNVLVGGGL AMTYGDITTY PRKASAFGYI STKDVLKIAE TVVTVQRDWG
     NRSDRRHAKT KYTLTRVGVT TFKSEVERRS GVQFHPIRPY VFTDRGDRFG WVQGIDDYWH
     LTLFIENGRI SNNDPHKLLK RGIAEVAQIH SGSFRLTANQ NLIISGVSKD NKLMIESTLR
     KYGVINDDIT PQRKASMACV AFPTCPLAMA EAERFLPKFV TKIEHIMSKY RLEKDAIILR
     VTGCPNSCAR AMLSEIGLTG RSIGRYNLYL GGNNIGTRIP RLYKENITEN DILNILDTTI
     SRWAQERNSQ ESYGDYVVRS GIVNAVINSE KDFYE
//