Reviewed, UniProtKB/Swiss-Prot Q493M6 (SYA_BLOPB)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	BPEN_174

Organism

Blochmannia pennsylvanicus (strain BPEN) [Complete proteome] [HAMAP]

Taxonomic identifier

291272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › ant endosymbionts › Candidatus Blochmannia

Protein attributes

Sequence length	879 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036
Subunit structure	Homotetramer By similarity. HAMAP MF_00036
Subcellular location	Cytoplasm HAMAP MF_00036.
Domain	The C-terminal C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 879	879	Alanyl-tRNA synthetase HAMAP MF_00036		PRO_0000075068

Sequences

	Sequence		Length	Mass (Da)
	Q493M6-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: AB11E160B35B1A04	FASTA	879	99,895
	10 20 30 40 50 60 MYKNIDDIRQ EFLNFFKKKG HKIISSSTLI PNSDQTLLFT NSGMNQFKNI FLGIDKPLFK 70 80 90 100 110 120 RVATAQRCVR AGGKHNDLNN VGYTERHLTF FEMLGNFSFG DYFKNEAIQF AWELLTDVNW 130 140 150 160 170 180 FNLSKNKLWV TVHNCDGESY DIWSNQIDIP NEHIVKIGSN TNDFCDSDNF WQMGNIGPCG 190 200 210 220 230 240 PCSEIFYDRG DRFQGGPPGS LEEFGERYVE IWNLVFIQFD RQENGRLLSL PMLSVDTGMG 250 260 270 280 290 300 LERITSILQG VNSNFSIDVF KKLISSISRI MKINDIYINR SVYVIADHIR ACSFLIQDGV 310 320 330 340 350 360 LPANEGRGYV LRRIIRRAIL HGKKLGVNDI FFYKLVSPLI KYMQHIADIL HEKKDFIAEI 370 380 390 400 410 420 LLSEEKLFQD TLKRGLELLE KQLIRLKGNC LNGEIAFNLY DTYGFPFELT QDVCLKRGIH 430 440 450 460 470 480 VDQEGFDQAM LTQKKYTKEL NKFDISYEKL SLCNVSSIFV GYDKLICKSR VMALFQNNKS 490 500 510 520 530 540 INKIYTKEEG IVILNNTPFY GESGGQIGDC GQLNTISGFF QVSNTKKYGQ TIGHLGIVNS 550 560 570 580 590 600 GTISVGEEIV AQVDQHKRKC ISLNHSATHL LRAALSQVLG SHVTQKGSLV NDRYLRFDFY 610 620 630 640 650 660 HHATMTTTQM NTVENIVNEQ IWKNAPITVD VMPIELARNS GAVILINKQY DKKVRVLKIG 670 680 690 700 710 720 NFSTELCGGT HAEHTGEIGV FMITKECSIA AGIRRVEAVT NNVALSAIHQ YKMLLKNIFQ 730 740 750 760 770 780 STQSDDKNIL SKIHEFKCRY QKLEKEIDTL RNQQIIQQSL SFIKDVYYIK DVRILVKQVV 790 800 810 820 830 840 NMTSKTLFNM TDCIKHQLKS GVIVLINLNT NKKINLVVAV TKDLIERNRI NALSLVHYII 850 860 870 KILGGKGGGR PDFAQAGINN MATTSKISIE VNSLLHNIL

References

[1]	"Genome sequence of Blochmannia pennsylvanicus indicates parallel evolutionary trends among bacterial mutualists of insects." Degnan P.H., Lazarus A.B., Wernegreen J.J. Genome Res. 15:1023-1033(2005) [PubMed: 16077009] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000016 Genomic DNA. Translation: AAZ40814.1.
RefSeq	YP_277687.1.
3D structure databases
SMR	Q493M6. Positions 4-465, 488-699.
ModBase	Search...
Protein-protein interaction databases
STRING	Q493M6.
Genome annotation databases
GeneID	3562669.
GenomeReviews	Gene locus BPEN_174 in contig CP000016_GR.
KEGG	bpn:BPEN_174.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0013.
HOGENOM	HBG354397.
OMA	VILEMES.
Enzyme and pathway databases
BioCyc	CBLO291272:BPEN_174-MONOMER.
Family and domain databases
HAMAP	MF_00036_B. Ala_tRNA_synth_B. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR003156. Pesterase_DHHA1. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_BLOPB

Accession

Primary (citable) accession number: Q493M6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	September 13, 2005
Last modified:	February 9, 2010
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)