Reviewed, UniProtKB/Swiss-Prot Q493M1 (TRMD_BLOPB)

Names and origin

Protein names

Recommended name:
    tRNA (guanine-N(1)-)-methyltransferase
    EC=2.1.1.31
Alternative name(s):
    M1G-methyltransferase
    tRNA [GM37] methyltransferase

Gene names

Name:	trmD
Ordered Locus Names:	BPEN_181

Organism

Blochmannia pennsylvanicus (strain BPEN) [Complete proteome] [HAMAP]

Taxonomic identifier

291272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › ant endosymbionts › Candidatus Blochmannia

Protein attributes

Sequence length	255 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP MF_00605
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(1)-methylguanine. HAMAP MF_00605
Subunit structure	Homodimer By similarity. HAMAP MF_00605
Subcellular location	Cytoplasm Potential HAMAP MF_00605.
Sequence similarities	Belongs to the RNA methyltransferase trmD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 255	255	tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605		PRO_0000257392
Regions
	Region	134 – 139	6	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	114	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q493M1-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: CE8090DD979E3E68	FASTA	255	29,421
	10 20 30 40 50 60 MLLGVITLFP DMFQSIVRYG IVGRAIRRGI LSIKLWNPRL FTYDRHHSVD ARPYGGGPGM 70 80 90 100 110 120 LMMIEPLRNA INQAKDELGN NIKVIYLSPQ GRKLKQKYVY KLAYDHQKLI LVCGRYQGID 130 140 150 160 170 180 ERLIQTEIDE EWSIGDYILS GGELAAMVLI DTISRVLPGV LGNQDSKESD SFSKERLDCP 190 200 210 220 230 240 HYTRPETFDG MKVPSVLLSG NHDEIHRWKQ KQALGRTWIK RPDLLNYIQL TNEEKNLLSE 250 FKNEYLLSLN KKTRK

References

[1]	"Genome sequence of Blochmannia pennsylvanicus indicates parallel evolutionary trends among bacterial mutualists of insects." Degnan P.H., Lazarus A.B., Wernegreen J.J. Genome Res. 15:1023-1033(2005) [PubMed: 16077009] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000016 Genomic DNA. Translation: AAZ40819.1.
RefSeq	YP_277692.1.
3D structure databases
SMR	Q493M1. Positions 1-245.
ModBase	Search...
Protein-protein interaction databases
STRING	Q493M1.
Genome annotation databases
GeneID	3562676.
GenomeReviews	Gene locus BPEN_181 in contig CP000016_GR.
KEGG	bpn:BPEN_181.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0336.
HOGENOM	HBG285805.
OMA	HRSVDDT.
Enzyme and pathway databases
BioCyc	CBLO291272:BPEN_181-MONOMER.
Family and domain databases
HAMAP	MF_00605. TrmD. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. [Graphical view]
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
TIGRFAMs	TIGR00088. trmD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMD_BLOPB

Accession

Primary (citable) accession number: Q493M1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	September 13, 2005
Last modified:	February 9, 2010
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)