Reviewed, UniProtKB/Swiss-Prot Q493L3 (MURB_BLOPB)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylenolpyruvoylglucosamine reductase
    EC=1.1.1.158
Alternative name(s):
    UDP-N-acetylmuramate dehydrogenase

Gene names

Name:	murB
Ordered Locus Names:	BPEN_189

Organism

Blochmannia pennsylvanicus (strain BPEN) [Complete proteome] [HAMAP]

Taxonomic identifier

291272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › ant endosymbionts › Candidatus Blochmannia

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00037
Catalytic activity	UDP-N-acetylmuramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH. HAMAP MF_00037
Cofactor	FAD By similarity. HAMAP MF_00037
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00037
Subcellular location	Cytoplasm Probable HAMAP MF_00037.
Sequence similarities	Belongs to the murB family. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro UDP-N-acetylmuramate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 345	345	UDP-N-acetylenolpyruvoylglucosamine reductase HAMAP MF_00037		PRO_0000224667
Regions
	Domain	16 – 185	170	FAD-binding PCMH-type
Sites
	Active site	162	1	By similarity
	Active site	231	1	Proton donor By similarity
	Active site	328	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q493L3-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: FDD5BE20F6E58CA2	FASTA	345	39,501
	10 20 30 40 50 60 MYEHPFQLKS LNTFSVNAFA KSVVTAHHEH TLLKFWRKAY RKGKPVLILG GGSNILFLEN 70 80 90 100 110 120 YSGTILLNRI KGIFITENET AWQLHVGAGE KWDELVVHTI KKNIPGLENL ACIPGYVGAA 130 140 150 160 170 180 PIQNIGAYGV ELSQICEYVD AIDLYSGKKI RFTCSECDFK YRDSIFRNCL EKYAIVSVGL 190 200 210 220 230 240 RLCKKWKPIL DYHELAHLEK FHITPRQIFN FIYIIRHKKL PDPVLVGNAG SFFKNPIIDI 250 260 270 280 290 300 KTARCLFQIY PNMPYFYQKD GRIKLSAGWL IEYCQLKGYI FGEAAIYPKQ ALVLINSKKI 310 320 330 340 ATGTEIAALA LYIYNKVADQ FNIYLQPEVR LIGNYGEINP KKLFM

References

[1]	"Genome sequence of Blochmannia pennsylvanicus indicates parallel evolutionary trends among bacterial mutualists of insects." Degnan P.H., Lazarus A.B., Wernegreen J.J. Genome Res. 15:1023-1033(2005) [PubMed: 16077009] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000016 Genomic DNA. Translation: AAZ40827.1.
RefSeq	YP_277700.1.
3D structure databases
SMR	Q493L3. Positions 7-342.
ModBase	Search...
Protein-protein interaction databases
STRING	Q493L3.
Genome annotation databases
GeneID	3562908.
GenomeReviews	Gene locus BPEN_189 in contig CP000016_GR.
KEGG	bpn:BPEN_189.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0812.
HOGENOM	HBG686573.
OMA	WLIDQCG.
Enzyme and pathway databases
BioCyc	CBLO291272:BPEN_189-MONOMER.
Family and domain databases
HAMAP	MF_00037. MurB. [Tree]
InterPro	IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR016166. FAD-bd_2. IPR016167. FAD-bd_2_sub1. IPR003170. MurB. IPR011601. MurB_C. IPR006094. Oxid_FAD_bind_N. [Graphical view]
Gene3D	G3DSA:3.30.465.10. CO_DH_flavoprot_FAD-bd_sub2. 1 hit. G3DSA:3.30.43.10. FAD-binding_2_sub1. 1 hit. G3DSA:3.90.78.10. MurB_C. 1 hit.
PANTHER	PTHR21071. MurB. 1 hit.
Pfam	PF01565. FAD_binding_4. 1 hit. PF02873. MurB_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00179. murB. 1 hit.
PROSITE	PS51387. FAD_PCMH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MURB_BLOPB

Accession

Primary (citable) accession number: Q493L3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	September 13, 2005
Last modified:	February 9, 2010
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)