Reviewed, UniProtKB/Swiss-Prot Q493G7 (DXS_BLOPB)

Names and origin

Protein names

Recommended name:
    1-deoxy-D-xylulose-5-phosphate synthase
    EC=2.2.1.7
Alternative name(s):
    1-deoxyxylulose-5-phosphate synthase
      Short name=DXP synthase
      Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	BPEN_244

Organism

Blochmannia pennsylvanicus (strain BPEN) [Complete proteome] [HAMAP]

Taxonomic identifier

291272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › ant endosymbionts › Candidatus Blochmannia

Protein attributes

Sequence length	624 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity. HAMAP MF_00315
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP thiamin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 624	624	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_0000256383

Sequences

	Sequence		Length	Mass (Da)
	Q493G7-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: ECC8A6D77BE10E66	FASTA	624	68,959
	10 20 30 40 50 60 MNCNSNKYPI LGLINTPKEL RQLSEDHLAK LCNELRQFLL TSVSKSSGHF ASGLGTIELT 70 80 90 100 110 120 VALHYVYNTP FDYLIWDVGH QAYPHKILTG RRERIFSIRR KNGLHPFPCR DESEYDVLSV 130 140 150 160 170 180 GHSSTSISAG LGLAIAAERE MLGRRTVCVI GDGAITAGMA FEAMNHAGFT KSDLLIILND 190 200 210 220 230 240 NEMSISENVG ALNNHHTHIL SKKVYSNLKV DNNKILSDVL STNTLIKHIG NQIKNFNGTS 250 260 270 280 290 300 SSLFSQLGFN YIGPVNGHDV LELVYILRNI RDMKGPQFLH IITKKGCGYE PAEKDPIKWH 310 320 330 340 350 360 AVPKFNPESG SLPVEHSKNI TYSAIFGDWL CQVAARDNKI IGITPAMREG SGMSIFSQKY 370 380 390 400 410 420 PKQYFDVAIA EQHAVTFAAG LAIAGYKPIV AIYSTFLQRA YDQVIHDVAI QNLPVLFAVD 430 440 450 460 470 480 RGGIVGADGQ THQGAFDLSY LRCIPNMIIM TPSDACECKL MLYTGYRYRY GPSVVRYPKG 490 500 510 520 530 540 YAVPGNLADT TKLYTLPLSK GVIRRQGNCI AILNFGTLLQ SAYNVASKLN ATLVDMRFVK 550 560 570 580 590 600 PLDENLIKTL AKNHQVLITL EENTVMGGAG SGVNEFIMQN KLSIPVLNIG LPDFFISQGS 610 620 QSEILSELGL DSIGIYKKII KWIH

References

[1]	"Genome sequence of Blochmannia pennsylvanicus indicates parallel evolutionary trends among bacterial mutualists of insects." Degnan P.H., Lazarus A.B., Wernegreen J.J. Genome Res. 15:1023-1033(2005) [PubMed: 16077009] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000016 Genomic DNA. Translation: AAZ40875.1.
RefSeq	YP_277748.1.
3D structure databases
SMR	Q493G7. Positions 7-623.
ModBase	Search...
Protein-protein interaction databases
STRING	Q493G7.
Genome annotation databases
GeneID	3562778.
GenomeReviews	Gene locus BPEN_244 in contig CP000016_GR.
KEGG	bpn:BPEN_244.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1154.
HOGENOM	HBG571647.
OMA	QRFPDRY.
Enzyme and pathway databases
BioCyc	CBLO291272:BPEN_244-MONOMER.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR005477. Dxylulose-5-P_synthase. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR020826. Transketolase_BS. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
Pfam	PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. PF00456. Transketolase_N. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
TIGRFAMs	TIGR00204. dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_BLOPB

Accession

Primary (citable) accession number: Q493G7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	September 13, 2005
Last modified:	February 9, 2010
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)