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Q492Z3 (SYL_BLOPB) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:BPEN_321
OrganismBlochmannia pennsylvanicus (strain BPEN) [Complete proteome] [HAMAP]
Taxonomic identifier291272 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeant endosymbiontsCandidatus Blochmannia

Protein attributes

Sequence length872 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 872872Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009297

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif631 – 6355"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6341ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q492Z3 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: DDAEEB6764D24CFC

FASTA872100,838
        10         20         30         40         50         60 
MRKSYSPSDI ESVVQKHWYK NKTFAVIEDS NKEKYYCLSM IPYPSGNLHM GHVRNYTIGD 

        70         80         90        100        110        120 
VISRYQRMLG KNVLQPIGWD AFGLPAEHAA IKNNTDPSIW TYSNINYMKS QLQSLGFAYD 

       130        140        150        160        170        180 
WNRELITCHP DYYRWEQWFF TVLYKKGLVY KKTASVNWCS YHKTVLANEQ VTNNCCWRCH 

       190        200        210        220        230        240 
TPVKYKRIPQ WFIRITNYAD QLLHGLDQLT HWPEQVKIMQ RNWIGRSKGV NVTFRIENSN 

       250        260        270        280        290        300 
DTLTIYMTRL DIFMGITYLV ISTDHPIALQ VAKTDLNVAH FIQKNDDFYT KLNKKNVFYF 

       310        320        330        340        350        360 
EKMGMPTHIY AIHPITNSKL PIWVANFVIP MECDGMGAAI SIPAHNQQDW EFAYKYNLPI 

       370        380        390        400        410        420 
KPVIKNLDAI EPNIAMQAQE TTCDGILFNS GEFDGLSSCT ASNAIVKSLI ARGVAQYKVN 

       430        440        450        460        470        480 
YRLKDWGISR QRYWGVPIPM VTLSNGVVKP VLLDRLPVIL PKNMSTIHRS NDGYVDNSLK 

       490        500        510        520        530        540 
MYPNWIQTTY KGQAAIRDTD TFDTFMESSW YYARYTCPRY NDAMLNVHAA NYWLPVDQYI 

       550        560        570        580        590        600 
GGIEHAIMHL LYFRFYHKLM RDEGLVYSDE PAIRLLCQGM VLADSFYYVS PNGQHIWVDP 

       610        620        630        640        650        660 
THVTIKRDRI GGIVKAIDKD GRDLIYDGMC KMSKSKNNGI DPNIIIEKYG ADAVRFFIMF 

       670        680        690        700        710        720 
AAPIEAPLEW KESGIEGAQR FLKRIWNLIY HHIQDGPVDA LSVVILNHAQ KFIRYNVHKT 

       730        740        750        760        770        780 
IEKVTDDIDR RQSFNTALSA IMKLVKKLYN APKTSMQDRA VLQEALLVIV RLLYPFTPHI 

       790        800        810        820        830        840 
SFILWKALGG SEDIDNATWP IVDTQAIQND RTLVLVQING KMRHKVFAPL NSDKNVVYKL 

       850        860        870 
LETEGVLNKY LIGKKINNII YVPNRVINII AK 

« Hide

References

[1]"Genome sequence of Blochmannia pennsylvanicus indicates parallel evolutionary trends among bacterial mutualists of insects."
Degnan P.H., Lazarus A.B., Wernegreen J.J.
Genome Res. 15:1023-1033(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: BPEN.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000016 Genomic DNA. Translation: AAZ40951.1.
RefSeqYP_277824.1. NC_007292.1.

3D structure databases

ProteinModelPortalQ492Z3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING291272.BPEN_321.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAZ40951; AAZ40951; BPEN_321.
GeneID3562833.
KEGGbpn:BPEN_321.
PATRIC31965543. VBICanBlo110049_0312.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_BLOPB
AccessionPrimary (citable) accession number: Q492Z3
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 13, 2005
Last modified: April 16, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries