Q492Z3 (SYL_BLOPB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Leucine--tRNA ligase
EC=6.1.1.4

Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS

Gene names

Name:	leuS
Ordered Locus Names:	BPEN_321

Organism

Blochmannia pennsylvanicus (strain BPEN) [Complete proteome] [HAMAP]

Taxonomic identifier

291272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › ant endosymbionts › Candidatus Blochmannia

Protein attributes

Sequence length	872 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP MF_00049_B
Subcellular location	Cytoplasm HAMAP MF_00049_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	leucyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW leucine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 872	872	Leucine--tRNA ligase HAMAP MF_00049_B		PRO_1000009297
Regions
	Motif	42 – 52	11	"HIGH" region HAMAP MF_00049_B
	Motif	631 – 635	5	"KMSKS" region HAMAP MF_00049_B
Sites
	Binding site	634	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q492Z3 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: DDAEEB6764D24CFC	FASTA	872	100,838
	10 20 30 40 50 60 MRKSYSPSDI ESVVQKHWYK NKTFAVIEDS NKEKYYCLSM IPYPSGNLHM GHVRNYTIGD 70 80 90 100 110 120 VISRYQRMLG KNVLQPIGWD AFGLPAEHAA IKNNTDPSIW TYSNINYMKS QLQSLGFAYD 130 140 150 160 170 180 WNRELITCHP DYYRWEQWFF TVLYKKGLVY KKTASVNWCS YHKTVLANEQ VTNNCCWRCH 190 200 210 220 230 240 TPVKYKRIPQ WFIRITNYAD QLLHGLDQLT HWPEQVKIMQ RNWIGRSKGV NVTFRIENSN 250 260 270 280 290 300 DTLTIYMTRL DIFMGITYLV ISTDHPIALQ VAKTDLNVAH FIQKNDDFYT KLNKKNVFYF 310 320 330 340 350 360 EKMGMPTHIY AIHPITNSKL PIWVANFVIP MECDGMGAAI SIPAHNQQDW EFAYKYNLPI 370 380 390 400 410 420 KPVIKNLDAI EPNIAMQAQE TTCDGILFNS GEFDGLSSCT ASNAIVKSLI ARGVAQYKVN 430 440 450 460 470 480 YRLKDWGISR QRYWGVPIPM VTLSNGVVKP VLLDRLPVIL PKNMSTIHRS NDGYVDNSLK 490 500 510 520 530 540 MYPNWIQTTY KGQAAIRDTD TFDTFMESSW YYARYTCPRY NDAMLNVHAA NYWLPVDQYI 550 560 570 580 590 600 GGIEHAIMHL LYFRFYHKLM RDEGLVYSDE PAIRLLCQGM VLADSFYYVS PNGQHIWVDP 610 620 630 640 650 660 THVTIKRDRI GGIVKAIDKD GRDLIYDGMC KMSKSKNNGI DPNIIIEKYG ADAVRFFIMF 670 680 690 700 710 720 AAPIEAPLEW KESGIEGAQR FLKRIWNLIY HHIQDGPVDA LSVVILNHAQ KFIRYNVHKT 730 740 750 760 770 780 IEKVTDDIDR RQSFNTALSA IMKLVKKLYN APKTSMQDRA VLQEALLVIV RLLYPFTPHI 790 800 810 820 830 840 SFILWKALGG SEDIDNATWP IVDTQAIQND RTLVLVQING KMRHKVFAPL NSDKNVVYKL 850 860 870 LETEGVLNKY LIGKKINNII YVPNRVINII AK

References

[1]

"Genome sequence of Blochmannia pennsylvanicus indicates parallel evolutionary trends among bacterial mutualists of insects." Degnan P.H., Lazarus A.B., Wernegreen J.J. Genome Res. 15:1023-1033(2005) [PubMed: 16077009] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BPEN.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000016 Genomic DNA. Translation: AAZ40951.1.
RefSeq	YP_277824.1. NC_007292.1.
3D structure databases
HSSP	HSSP built from PDB template 2AJH based on UniProtKB P07813.
ProteinModelPortal	Q492Z3.
ModBase	Search...
Protein-protein interaction databases
STRING	Q492Z3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3562833.
GenomeReviews	Gene locus BPEN_321 in contig CP000016_GR.
KEGG	bpn:BPEN_321.
PATRIC	31965543. VBICanBlo110049_0312.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0495.
HOGENOM	HBG693845.
OMA	YARYCSP.
PhylomeDB	Q492Z3.
ProtClustDB	PRK00390.
Enzyme and pathway databases
BioCyc	CBLO291272:BPEN_321-MONOMER.
Family and domain databases
HAMAP	MF_00049_B. Leu_tRNA_synth_B. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002302. Leu-tRNA-synth_Ia_bac/mito. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. [Graphical view]
Gene3D	G3DSA:3.90.740.10. G3DSA:3.90.740.10. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 3 hits.
KO	K01869.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 4 hits. [Graphical view]
PRINTS	PR00985. TRNASYNTHLEU.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit. SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMs	TIGR00396. LeuS_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYL_BLOPB

Accession

Primary (citable) accession number: Q492Z3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 13, 2005
Last modified:	January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program