Reviewed, UniProtKB/Swiss-Prot Q492W0 (ISPE_BLOPB)

Names and origin

Protein names

Recommended name:
    4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
      Short name=CMK
    EC=2.7.1.148
Alternative name(s):
    4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	BPEN_357

Organism

Blochmannia pennsylvanicus (strain BPEN) [Complete proteome] [HAMAP]

Taxonomic identifier

291272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › ant endosymbionts › Candidatus Blochmannia

Protein attributes

Sequence length	295 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity.
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphorylation Inferred from electronic annotation. Source: InterPro terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 295	295	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_0000235070
Regions
	Nucleotide binding	97 – 107	11	ATP Potential
Sites
	Active site	10	1	By similarity
	Active site	139	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q492W0-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: B8562306D1D4C54A	FASTA	295	33,750
	10 20 30 40 50 60 MNYQWPSPGK LNLFLYVTGC RADGYHYLQT LFQLIEYGDT IKIFVTNNGR IRLFTIMNDL 70 80 90 100 110 120 VCRDNLIIRA AKLLQYYCWP NKKPVFGADI FLDKILPIGS GLGGASSNAA TVLMVLNQQW 130 140 150 160 170 180 RCYLNKHVLM HLGLMLGADV PFFLYGRSAF AEGIGNILIP VFVPKKWYLI LIPPVRISTF 190 200 210 220 230 240 WGFQMYELEN HCYSPFRSMR ELLSVSFHND FEEIIKKISP EIKIFFACLS QFALARLTGT 250 260 270 280 290 GSCIFAEFKT EQLAYQVQSY LPSWVSSIIT RGINLSPLHQ KLLKCTAYSS IIFLS

References

[1]	"Genome sequence of Blochmannia pennsylvanicus indicates parallel evolutionary trends among bacterial mutualists of insects." Degnan P.H., Lazarus A.B., Wernegreen J.J. Genome Res. 15:1023-1033(2005) [PubMed: 16077009] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000016 Genomic DNA. Translation: AAZ40982.1.
RefSeq	YP_277857.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q492W0.
Genome annotation databases
GeneID	3562814.
GenomeReviews	Gene locus BPEN_357 in contig CP000016_GR.
KEGG	bpn:BPEN_357.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q492W0.
OMA	PMGGGIG.
Enzyme and pathway databases
BioCyc	CBLO291272:BPEN_357-MON.
Family and domain databases
HAMAP	MF_00061. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. [Graphical view]
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
TIGRFAMs	TIGR00154. ispE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_BLOPB

Accession

Primary (citable) accession number: Q492W0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	September 13, 2005
Last modified:	November 3, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)