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Reviewed, UniProtKB/Swiss-Prot Q492J8 (SYM_BLOPB)

Last modified February 9, 2010. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Methionyl-tRNA synthetase
    EC=6.1.1.10
Alternative name(s):
    Methionine--tRNA ligase
      Short name=MetRS
Gene names
Name: metG
Ordered Locus Names: BPEN_486
OrganismBlochmannia pennsylvanicus (strain BPEN) [Complete proteome] [HAMAP]
Taxonomic identifier291272 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeant endosymbiontsCandidatus Blochmannia

Protein attributes

Sequence length555 AA.
Sequence statusComplete.
Protein existenceInferred from homology.

General annotation (Comments)

Function

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP MF_00098

Catalytic activity

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_00098

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP MF_00098

Subunit structure

Monomer By similarity. HAMAP MF_00098

Subcellular location

Cytoplasm By similarity HAMAP MF_00098.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processmethionyl-tRNA aminoacylation

Inferred from electronic annotation. Source: HAMAP

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: HAMAP

methionine-tRNA ligase activity

Inferred from electronic annotation. Source: HAMAP

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 555555Methionyl-tRNA synthetase HAMAP MF_00098
PRO_0000331784

Regions

Motif13 – 2311"HIGH" region HAMAP MF_00098
Motif330 – 3345"KMSKS" region HAMAP MF_00098

Sites

Metal binding1441Zinc By similarity
Metal binding1471Zinc By similarity
Metal binding1571Zinc By similarity
Metal binding1601Zinc By similarity
Binding site3331ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q492J8-1 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: 6A7A4215AAF8BB56

FASTA55564,541
        10         20         30         40         50         60 
MTTKKMLVTC ALPYANGSLH IGHMLEHIQA DIWVRYQRMQ GNCVYFICAD DAHGTAIMLK 

        70         80         90        100        110        120 
SQQLNIAPEQ MIAQIRQEHQ RDCYKFGISY DNYYSTHSDE TRELLHDIYS RLNTRGFIKS 

       130        140        150        160        170        180 
KFISQLYDSK KNMFLPDRFV KGICPKCKKD DQYGDNCAAC GTIYTSLELI NPKSVISGTS 

       190        200        210        220        230        240 
PIIRKSEHLF FDLPAFTDTL RTWIRSGSIQ KEVANKVEEW FKLGLKKWDI SRDAPYFGFK 

       250        260        270        280        290        300 
IPNSSEKYFY VWMDAPVGYM GTFKNLCKKN KNISFNDFWG SNSKTDLYHF IGKDIIYFHC 

       310        320        330        340        350        360 
LFWPAVLSGS QFRKPTNIFV HGHVTLNGSK ISKSKGTCIN VSTYLSCLNP DYLRYYYATK 

       370        380        390        400        410        420 
LSSHANDIDL NLSDFITRVN SDIINKILNL ASRNSGFIHQ YYNGHLSNTL THPLIYNMFI 

       430        440        450        460        470        480 
ESRHYIGKLF QKREFNYAMR EIMKLADEAN RYIDKHAPWH IAKKIDRRQE ALSIYSMGIQ 

       490        500        510        520        530        540 
LFRVLMIYLK PVLPKLANYS ECFLNTRLTW GSLSAPLSNH RINKFKIIFS RIHPDQIASM 

       550 
TNKSQLHERL TDNNV 

« Hide

References

[1]"Genome sequence of Blochmannia pennsylvanicus indicates parallel evolutionary trends among bacterial mutualists of insects."
Degnan P.H., Lazarus A.B., Wernegreen J.J.
Genome Res. 15:1023-1033(2005) [PubMed: 16077009] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000016 Genomic DNA. Translation: AAZ41101.1.
RefSeqYP_277978.1.

3D structure databases

SMRQ492J8. Positions 4-545.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ492J8.

Genome annotation databases

GeneID3562993.
GenomeReviewsGene locus BPEN_486 in contig CP000016_GR.
KEGGbpn:BPEN_486.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0143.
HOGENOMHBG721667.
OMADFSWREF.

Enzyme and pathway databases

BioCycCBLO291272:BPEN_486-MONOMER.

Family and domain databases

HAMAPMF_00098. Met_tRNA_synth_type1.
[Tree]
InterProIPR015413. aa-tRNA-synt_I.
IPR001412. aa-tRNA-synth_I_CS.
IPR002304. Met-tRNA-synth_Ia.
IPR014729. Rossmann-like_a/b/a_fold.
IPR014758. tRNA-synt_met_N.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
Gene3DG3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PfamPF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR01041. TRNASYNTHMET.
TIGRFAMsTIGR00398. metG. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYM_BLOPB
AccessionPrimary (citable) accession number: Q492J8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: September 13, 2005
Last modified: February 9, 2010
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents