Reviewed, UniProtKB/Swiss-Prot Q492J8 (SYM_BLOPB)

Names and origin

Protein names

Recommended name:
    Methionyl-tRNA synthetase
    EC=6.1.1.10
Alternative name(s):
    Methionine--tRNA ligase
      Short name=MetRS

Gene names

Name:	metG
Ordered Locus Names:	BPEN_486

Organism

Blochmannia pennsylvanicus (strain BPEN) [Complete proteome] [HAMAP]

Taxonomic identifier

291272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › ant endosymbionts › Candidatus Blochmannia

Protein attributes

Sequence length	555 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP MF_00098
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_00098
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00098
Subunit structure	Monomer By similarity. HAMAP MF_00098
Subcellular location	Cytoplasm By similarity HAMAP MF_00098.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP methionine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 555	555	Methionyl-tRNA synthetase HAMAP MF_00098		PRO_0000331784
Regions
	Motif	13 – 23	11	"HIGH" region HAMAP MF_00098
	Motif	330 – 334	5	"KMSKS" region HAMAP MF_00098
Sites
	Metal binding	144	1	Zinc By similarity
	Metal binding	147	1	Zinc By similarity
	Metal binding	157	1	Zinc By similarity
	Metal binding	160	1	Zinc By similarity
	Binding site	333	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q492J8-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 6A7A4215AAF8BB56	FASTA	555	64,541
	10 20 30 40 50 60 MTTKKMLVTC ALPYANGSLH IGHMLEHIQA DIWVRYQRMQ GNCVYFICAD DAHGTAIMLK 70 80 90 100 110 120 SQQLNIAPEQ MIAQIRQEHQ RDCYKFGISY DNYYSTHSDE TRELLHDIYS RLNTRGFIKS 130 140 150 160 170 180 KFISQLYDSK KNMFLPDRFV KGICPKCKKD DQYGDNCAAC GTIYTSLELI NPKSVISGTS 190 200 210 220 230 240 PIIRKSEHLF FDLPAFTDTL RTWIRSGSIQ KEVANKVEEW FKLGLKKWDI SRDAPYFGFK 250 260 270 280 290 300 IPNSSEKYFY VWMDAPVGYM GTFKNLCKKN KNISFNDFWG SNSKTDLYHF IGKDIIYFHC 310 320 330 340 350 360 LFWPAVLSGS QFRKPTNIFV HGHVTLNGSK ISKSKGTCIN VSTYLSCLNP DYLRYYYATK 370 380 390 400 410 420 LSSHANDIDL NLSDFITRVN SDIINKILNL ASRNSGFIHQ YYNGHLSNTL THPLIYNMFI 430 440 450 460 470 480 ESRHYIGKLF QKREFNYAMR EIMKLADEAN RYIDKHAPWH IAKKIDRRQE ALSIYSMGIQ 490 500 510 520 530 540 LFRVLMIYLK PVLPKLANYS ECFLNTRLTW GSLSAPLSNH RINKFKIIFS RIHPDQIASM 550 TNKSQLHERL TDNNV

References

[1]	"Genome sequence of Blochmannia pennsylvanicus indicates parallel evolutionary trends among bacterial mutualists of insects." Degnan P.H., Lazarus A.B., Wernegreen J.J. Genome Res. 15:1023-1033(2005) [PubMed: 16077009] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000016 Genomic DNA. Translation: AAZ41101.1.
RefSeq	YP_277978.1.
3D structure databases
SMR	Q492J8. Positions 4-545.
ModBase	Search...
Protein-protein interaction databases
STRING	Q492J8.
Genome annotation databases
GeneID	3562993.
GenomeReviews	Gene locus BPEN_486 in contig CP000016_GR.
KEGG	bpn:BPEN_486.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0143.
HOGENOM	HBG721667.
OMA	DFSWREF.
Enzyme and pathway databases
BioCyc	CBLO291272:BPEN_486-MONOMER.
Family and domain databases
HAMAP	MF_00098. Met_tRNA_synth_type1. [Tree]
InterPro	IPR015413. aa-tRNA-synt_I. IPR001412. aa-tRNA-synth_I_CS. IPR002304. Met-tRNA-synth_Ia. IPR014729. Rossmann-like_a/b/a_fold. IPR014758. tRNA-synt_met_N. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF09334. tRNA-synt_1g. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
TIGRFAMs	TIGR00398. metG. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_BLOPB

Accession

Primary (citable) accession number: Q492J8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	September 13, 2005
Last modified:	February 9, 2010
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)