Reviewed, UniProtKB/Swiss-Prot Q492J5 (DDL_BLOPB)

Names and origin

Protein names

Recommended name:
    D-alanine--D-alanine ligase
    EC=6.3.2.4
Alternative name(s):
    D-alanylalanine synthetase
    D-Ala-D-Ala ligase

Gene names

Name:	ddl
Ordered Locus Names:	BPEN_490

Organism

Blochmannia pennsylvanicus (strain BPEN) [Complete proteome] [HAMAP]

Taxonomic identifier

291272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › ant endosymbionts › Candidatus Blochmannia

Protein attributes

Sequence length	368 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP MF_00047
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00047
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the D-alanine--D-alanine ligase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell wall Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro D-alanine-D-alanine ligase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 368	368	D-alanine--D-alanine ligase HAMAP MF_00047		PRO_0000341060
Regions
	Domain	137 – 340	204	ATP-grasp
	Nucleotide binding	167 – 222	56	ATP By similarity
Sites
	Metal binding	294	1	Magnesium or manganese 1 By similarity
	Metal binding	307	1	Magnesium or manganese 1 By similarity
	Metal binding	307	1	Magnesium or manganese 2 By similarity
	Metal binding	309	1	Magnesium or manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q492J5-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: F4BD5CA22F70A2E4	FASTA	368	41,585
	10 20 30 40 50 60 MSKLCIGIIC GGRSLEHEIS LKSAMCIAQF IDKFRFEAMI LWIDKKGCWH LKDIDFNDLS 70 80 90 100 110 120 YHKDEKISIL LQNCPYPFKF HTKNINILLK FDVIFPVVHG TLGEDGALQG LLCMMNLPFV 130 140 150 160 170 180 GSNVLSSSVG MDKDVSKCLL RDAGLSVVPF RTVLAQDQHN IDFDYFVSIF GLPLFVKPSN 190 200 210 220 230 240 QGSSIGVSKV TKCKDFNLAL IKAFSFSNKI LIEPAIIGRE LECAVLGNDN PKISVCGEVI 250 260 270 280 290 300 LSDNNFYTYH DKYVKRKAQI MIPALINDAI SDDIRHIALR AFQVLNCSGM ARVDVFLNLD 310 320 330 340 350 360 NKIFINEVNT LPGFTFDSMY PKLWEVSGLN LQELITKLVE LALDLHDKKN CFYHVDGAVL KYFRHERI

References

[1]	"Genome sequence of Blochmannia pennsylvanicus indicates parallel evolutionary trends among bacterial mutualists of insects." Degnan P.H., Lazarus A.B., Wernegreen J.J. Genome Res. 15:1023-1033(2005) [PubMed: 16077009] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000016 Genomic DNA. Translation: AAZ41104.1.
RefSeq	YP_277981.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3562997.
GenomeReviews	Gene locus BPEN_490 in contig CP000016_GR.
KEGG	bpn:BPEN_490.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q492J5.
Enzyme and pathway databases
BioCyc	CBLO291272:BPEN_490-MON.
Family and domain databases
HAMAP	MF_00047. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013816. ATP_grasp_subdomain_2. IPR000291. D-Ala_lig_Van_CS. IPR005905. D_ala_D_ala. IPR011095. Dala_Dala_lig_C. IPR011127. Dala_Dala_lig_N. IPR013817. Pre-ATP_grasp. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF07478. Dala_Dala_lig_C. 1 hit. PF01820. Dala_Dala_lig_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00843. DALA_DALA_LIGASE_1. 1 hit. PS00844. DALA_DALA_LIGASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DDL_BLOPB

Accession

Primary (citable) accession number: Q492J5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	September 13, 2005
Last modified:	November 25, 2008
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)