Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q492J5 (DDL_BLOPB) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:BPEN_490
OrganismBlochmannia pennsylvanicus (strain BPEN) [Complete proteome] [HAMAP]
Taxonomic identifier291272 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeant endosymbiontsCandidatus Blochmannia

Protein attributes

Sequence length368 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 368368D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000341060

Regions

Domain137 – 340204ATP-grasp
Nucleotide binding167 – 22256ATP By similarity

Sites

Metal binding2941Magnesium or manganese 1 By similarity
Metal binding3071Magnesium or manganese 1 By similarity
Metal binding3071Magnesium or manganese 2 By similarity
Metal binding3091Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q492J5 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: F4BD5CA22F70A2E4

FASTA36841,585
        10         20         30         40         50         60 
MSKLCIGIIC GGRSLEHEIS LKSAMCIAQF IDKFRFEAMI LWIDKKGCWH LKDIDFNDLS 

        70         80         90        100        110        120 
YHKDEKISIL LQNCPYPFKF HTKNINILLK FDVIFPVVHG TLGEDGALQG LLCMMNLPFV 

       130        140        150        160        170        180 
GSNVLSSSVG MDKDVSKCLL RDAGLSVVPF RTVLAQDQHN IDFDYFVSIF GLPLFVKPSN 

       190        200        210        220        230        240 
QGSSIGVSKV TKCKDFNLAL IKAFSFSNKI LIEPAIIGRE LECAVLGNDN PKISVCGEVI 

       250        260        270        280        290        300 
LSDNNFYTYH DKYVKRKAQI MIPALINDAI SDDIRHIALR AFQVLNCSGM ARVDVFLNLD 

       310        320        330        340        350        360 
NKIFINEVNT LPGFTFDSMY PKLWEVSGLN LQELITKLVE LALDLHDKKN CFYHVDGAVL 


KYFRHERI 

« Hide

References

[1]"Genome sequence of Blochmannia pennsylvanicus indicates parallel evolutionary trends among bacterial mutualists of insects."
Degnan P.H., Lazarus A.B., Wernegreen J.J.
Genome Res. 15:1023-1033(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: BPEN.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000016 Genomic DNA. Translation: AAZ41104.1.
RefSeqYP_277981.1. NC_007292.1.

3D structure databases

ProteinModelPortalQ492J5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING291272.BPEN_490.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAZ41104; AAZ41104; BPEN_490.
GeneID3562997.
KEGGbpn:BPEN_490.
PATRIC31965901. VBICanBlo110049_0478.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMAIECGVLG.
OrthoDBEOG64BQ73.

Enzyme and pathway databases

UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_BLOPB
AccessionPrimary (citable) accession number: Q492J5
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: September 13, 2005
Last modified: May 14, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways