Q492G6 (SYE_BLOPB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	BPEN_521

Organism

Blochmannia pennsylvanicus (strain BPEN) [Complete proteome] [HAMAP]

Taxonomic identifier

291272 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › unclassified Enterobacteriaceae › ant, tsetse, mealybug, aphid, etc. endosymbionts › ant endosymbionts › Candidatus Blochmannia › Candidatus Blochmannia pennsylvanicus

Protein attributes

Sequence length	468 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 468	468	Glutamate--tRNA ligase HAMAP-Rule MF_00022		PRO_0000237341
Regions
	Motif	9 – 19	11	"HIGH" region HAMAP-Rule MF_00022
	Motif	239 – 243	5	"KMSKS" region HAMAP-Rule MF_00022
Sites
	Binding site	242	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q492G6 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 22F09C0C03E75B6D	FASTA	468	54,323
	10 20 30 40 50 60 MNIKTRFAPS PTGSIHIGNI RTALYSWLFA RKQGGKFLLR IEDSDLQRSI DDTVELIVAG 70 80 90 100 110 120 MRWLSLDWDE GPYFQTNRFS RYNSIISYMI QHDMAYKCYC SSERLESLRS NQIKNGEKPK 130 140 150 160 170 180 YDGYCRFKST DIYSSSISSY VVRFCNPQDG VVIFHDQIRG TITFNNKELD DLIIRRADGS 190 200 210 220 230 240 PTYNFCVVID DMDMQITHII RGEEHINNTP RQINILKALR APIPTYAHVS MILDNNLKKL 250 260 270 280 290 300 SKRCGTLGIM QYRNDGFLPE AILNYLVRLG WSHGDQEIFS IEEMIKYFDL SRISKSPSIL 310 320 330 340 350 360 NLEKLLWLNH YYINHLPIDY VASHLSWHMH QQKINIQNGP KLTDIIKLFA RRSRTLKEIV 370 380 390 400 410 420 NNCLYFYIDF DLFDNKVAKD YLKPVAITPL KFLRKKFSNI VDWTPEIIKS IIIETVNEFN 430 440 450 460 TSIDKIGMPL RVALTGTNCS PTLSITIHAI GQSRVLERID QAIRYIST

References

[1]

"Genome sequence of Blochmannia pennsylvanicus indicates parallel evolutionary trends among bacterial mutualists of insects." Degnan P.H., Lazarus A.B., Wernegreen J.J. Genome Res. 15:1023-1033(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BPEN.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000016 Genomic DNA. Translation: AAZ41133.1.
RefSeq	YP_278010.1. NC_007292.1.
3D structure databases
ProteinModelPortal	Q492G6.
ModBase	Search...
Protein-protein interaction databases
STRING	291272.BPEN_521.
Proteomic databases
PRIDE	Q492G6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAZ41133; AAZ41133; BPEN_521.
GeneID	3562945.
KEGG	bpn:BPEN_521.
PATRIC	31965967. VBICanBlo110049_0509.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HOG000252722.
KO	K01885.
OMA	WENVRVR.
Family and domain databases
Gene3D	1.10.10.350. 1 hit. 1.10.1160.10. 1 hit. 3.40.50.620. 2 hits.
HAMAP	MF_00022_B. Glu_tRNA_synth_B.
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-ligase_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR10119. PTHR10119. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. gltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_BLOPB

Accession

Primary (citable) accession number: Q492G6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	September 13, 2005
Last modified:	May 1, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program