Reviewed, UniProtKB/Swiss-Prot Q492G5 (DNLJ_BLOPB)

Names and origin

Protein names

Recommended name:
    DNA ligase
    EC=6.5.1.2
Alternative name(s):
    Polydeoxyribonucleotide synthase [NAD+]

Gene names

Name:	ligA
Ordered Locus Names:	BPEN_524

Organism

Blochmannia pennsylvanicus (strain BPEN) [Complete proteome] [HAMAP]

Taxonomic identifier

291272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › ant endosymbionts › Candidatus Blochmannia

Protein attributes

Sequence length	595 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA By similarity. HAMAP MF_01588
Catalytic activity	NAD+ + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + nicotinamide nucleotide + (deoxyribonucleotide)(n+m). HAMAP MF_01588
Cofactor	Magnesium or manganese By similarity. HAMAP MF_01588
Sequence similarities	Belongs to the NAD-dependent DNA ligase family. LigA subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Ligand	Magnesium Manganese Metal-binding NAD Zinc
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (NAD+) activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 595	595	DNA ligase HAMAP MF_01588		PRO_0000313142
Regions
	Nucleotide binding	32 – 36	5	NAD By similarity
	Nucleotide binding	81 – 82	2	NAD By similarity
Sites
	Active site	115	1	N6-AMP-lysine intermediate By similarity
	Metal binding	414	1	Zinc By similarity
	Metal binding	417	1	Zinc By similarity
	Metal binding	432	1	Zinc By similarity
	Metal binding	438	1	Zinc By similarity
	Binding site	113	1	NAD By similarity
	Binding site	136	1	NAD By similarity
	Binding site	178	1	NAD By similarity
	Binding site	296	1	NAD By similarity
	Binding site	320	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q492G5-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: E882FA3B9410D61B	FASTA	595	67,382
	10 20 30 40 50 60 MKFVKQKIQK LRKKLRHWEY LYYTKNESAV SDEKYDAMLE KLNQLEQIYP HLIAESSPTQ 70 80 90 100 110 120 RIGGVSQYNF KKIHHKVPML SLNSIVASFQ LLSFDKRIKI KLHANHVMSY CCELKIDGVA 130 140 150 160 170 180 VSLLYKEGKL IYAATRGDGK IGEDVTENIS TIRAVPMCLK IDSNKYGKLP YLLEIRGEVF 190 200 210 220 230 240 ISKLCFLELN KITIQQGNKP FSNARNAASG SLRQLDPSVT ATRPLSFYCY GISNYCGEKE 250 260 270 280 290 300 LPDSHWERLQ LCENWGLPIN NYIRLISGVN KVLEYYSYIK TIRSNLEFNI DGIVIKVNSC 310 320 330 340 350 360 AYQSKLGCGS RAPHWALAYK FPSEVSSAKV DNVIFQVGRT GIITPIAYLE PIVISDVTIR 370 380 390 400 410 420 KVNMHNINEV KRLGLMIGDT VRIQRSGDVI PKIVEVILSE RTDHVKTIEL PRFCPVCGSR 430 440 450 460 470 480 IKTWRNQSIL RCTAGLSCLA QRKATLEHFV SKKAMNIYGM GNKIIDQLVN QGLIFTSSDV 490 500 510 520 530 540 FRLNKNKLLC LEGFGLENIE RLLRSIEDSK KITLARFIYA LGIYGVGETV ASNLAIVYKT 550 560 570 580 590 IENLAAADLQ SLSNLKYVGP IIANNIYHFF RNPDNLKNVQ DLIDPAIGIQ LYVIT

References

[1]	"Genome sequence of Blochmannia pennsylvanicus indicates parallel evolutionary trends among bacterial mutualists of insects." Degnan P.H., Lazarus A.B., Wernegreen J.J. Genome Res. 15:1023-1033(2005) [PubMed: 16077009] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000016 Genomic DNA. Translation: AAZ41134.1.
RefSeq	YP_278011.1.
3D structure databases
SMR	Q492G5. Positions 1-590.
ModBase	Search...
Protein-protein interaction databases
STRING	Q492G5.
Genome annotation databases
GeneID	3562951.
GenomeReviews	Gene locus BPEN_524 in contig CP000016_GR.
KEGG	bpn:BPEN_524.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0272.
HOGENOM	HBG620317.
OMA	SHELLCE.
Enzyme and pathway databases
BioCyc	CBLO291272:BPEN_524-MONOMER.
Family and domain databases
HAMAP	MF_01588. DNA_ligase_A. [Tree]
InterPro	IPR018239. DNA_ligase_AS. IPR004150. DNA_ligase_OB. IPR001679. DNAligase. IPR013839. DNAligase_adenylation. IPR013840. DNAligase_N. IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR010994. RuvA_2-like. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF01653. DNA_ligase_aden. 1 hit. PF03120. DNA_ligase_OB. 1 hit. [Graphical view]
PIRSF	PIRSF001604. LigA. 1 hit.
SMART	SM00278. HhH1. 4 hits. SM00532. LIGANc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00575. dnlj. 1 hit.
PROSITE	PS50172. BRCT. False negative. PS01055. DNA_LIGASE_N1. 1 hit. PS01056. DNA_LIGASE_N2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLJ_BLOPB

Accession

Primary (citable) accession number: Q492G5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 13, 2005
Last modified:	February 9, 2010
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)