Reviewed, UniProtKB/Swiss-Prot Q492F5 (DAPA_BLOPB)

Names and origin

Protein names

Recommended name:
    Dihydrodipicolinate synthase
      Short name=DHDPS
    EC=4.2.1.52

Gene names

Name:	dapA
Ordered Locus Names:	BPEN_536

Organism

Blochmannia pennsylvanicus (strain BPEN) [Complete proteome] [HAMAP]

Taxonomic identifier

291272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › ant endosymbionts › Candidatus Blochmannia

Protein attributes

Sequence length	299 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418
Subunit structure	Homotetramer By similarity. HAMAP MF_00418
Subcellular location	Cytoplasm By similarity HAMAP MF_00418.
Sequence similarities	Belongs to the DHDPS family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydrodipicolinate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 299	299	Dihydrodipicolinate synthase HAMAP MF_00418		PRO_1000050168
Regions
	Region	48 – 49	2	Pyruvate binding By similarity
Sites
	Active site	161	1	Schiff-base intermediate with substrate By similarity
	Binding site	106	1	Pyruvate By similarity
	Site	133	1	Involved in proton transfer during cleavage By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q492F5-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: A0CB7A7FBE0AD0E9	FASTA	299	32,701
	10 20 30 40 50 60 MFMGSIVALI TPMDLKGTVD RISLKKLVDH HVVSGTSAIV SVGTTGEMSG LTHEEHVDVV 70 80 90 100 110 120 MRTLEFSDGR LPVIAGTGAN STAEAVALTN KFNDSDIAAC LSVTPYYNRP NQEGLFQHFK 130 140 150 160 170 180 AISESTDLPQ ILYNVPIRTG CDMLPITVSR LAKIKNIVGI KEATGNLNRV NQLKQLVHED 190 200 210 220 230 240 FILLSGDDLS ALDFMKLGGV GVISVTANIA AKEMAELCKL ANENDFSTAQ HINQRLMPVH 250 260 270 280 290 QALFIDSNPI PVKWACKELG LISHYVLRLP MTVLSEVHRD VLKQALIDSG LFYNQSNYK

References

[1]	"Genome sequence of Blochmannia pennsylvanicus indicates parallel evolutionary trends among bacterial mutualists of insects." Degnan P.H., Lazarus A.B., Wernegreen J.J. Genome Res. 15:1023-1033(2005) [PubMed: 16077009] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000016 Genomic DNA. Translation: AAZ41146.1.
RefSeq	YP_278023.1.
3D structure databases
HSSP	HSSP built from PDB template 2A6N based on UniProtKB P0A6L2.
SMR	Q492F5. Positions 1-292.
ModBase	Search...
Protein-protein interaction databases
STRING	Q492F5.
Genome annotation databases
GeneID	3562873.
GenomeReviews	Gene locus BPEN_536 in contig CP000016_GR.
KEGG	bpn:BPEN_536.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0329.
HOGENOM	HBG358848.
OMA	TVEFAKG.
Enzyme and pathway databases
BioCyc	CBLO291272:BPEN_536-MONOMER.
Family and domain databases
HAMAP	MF_00418. DapA. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005263. DapA_synth. IPR002220. Dihydrodipicolinate_synth. IPR020625. Dihydrodipicolinate_synth_AS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR12128. DHDPS. 1 hit.
Pfam	PF00701. DHDPS. 1 hit. [Graphical view]
PRINTS	PR00146. DHPICSNTHASE.
TIGRFAMs	TIGR00674. dapA. 1 hit.
PROSITE	PS00665. DHDPS_1. False negative. PS00666. DHDPS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPA_BLOPB

Accession

Primary (citable) accession number: Q492F5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 13, 2005
Last modified:	February 9, 2010
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)