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Protein

Diaminopimelate epimerase

Gene

dapF

Organism
Blochmannia pennsylvanicus (strain BPEN)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.UniRule annotation

Catalytic activityi

LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

Pathwayi: L-lysine biosynthesis via DAP pathway

This protein is involved in step 1 of the subpathway that synthesizes DL-2,6-diaminopimelate from LL-2,6-diaminopimelate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Diaminopimelate epimerase (dapF)
This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes DL-2,6-diaminopimelate from LL-2,6-diaminopimelate, the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei11SubstrateUniRule annotation1
Binding sitei44SubstrateUniRule annotation1
Binding sitei64SubstrateUniRule annotation1
Active sitei73Proton donorUniRule annotation1
Binding sitei157SubstrateUniRule annotation1
Sitei159Could be important to modulate the pK values of the two catalytic cysteine residuesUniRule annotation1
Binding sitei190SubstrateUniRule annotation1
Sitei208Could be important to modulate the pK values of the two catalytic cysteine residuesUniRule annotation1
Active sitei217Proton acceptorUniRule annotation1
Sitei268Important for dimerizationUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processAmino-acid biosynthesis, Lysine biosynthesis

Enzyme and pathway databases

BioCyciCBLO291272:G1G3Y-601-MONOMER
UniPathwayiUPA00034; UER00025

Names & Taxonomyi

Protein namesi
Recommended name:
Diaminopimelate epimeraseUniRule annotation (EC:5.1.1.7UniRule annotation)
Short name:
DAP epimeraseUniRule annotation
Alternative name(s):
PLP-independent amino acid racemaseUniRule annotation
Gene namesi
Name:dapFUniRule annotation
Ordered Locus Names:BPEN_600
OrganismiBlochmannia pennsylvanicus (strain BPEN)
Taxonomic identifieri291272 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeant endosymbiontsCandidatus Blochmannia
Proteomesi
  • UP000007794 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000585401 – 274Diaminopimelate epimeraseAdd BLAST274

Proteomic databases

PRIDEiQ491Z7

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi291272.BPEN_600

Structurei

3D structure databases

ProteinModelPortaliQ491Z7
SMRiQ491Z7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni74 – 75Substrate bindingUniRule annotation2
Regioni208 – 209Substrate bindingUniRule annotation2
Regioni218 – 219Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the diaminopimelate epimerase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105E4Z Bacteria
COG0253 LUCA
HOGENOMiHOG000220466
KOiK01778
OMAiSMCGNGG
OrthoDBiPOG091H01QC

Family and domain databases

HAMAPiMF_00197 DAP_epimerase, 1 hit
InterProiView protein in InterPro
IPR018510 DAP_epimerase_AS
IPR001653 DAP_epimerase_DapF
PANTHERiPTHR31689 PTHR31689, 1 hit
PfamiView protein in Pfam
PF01678 DAP_epimerase, 2 hits
TIGRFAMsiTIGR00652 DapF, 1 hit
PROSITEiView protein in PROSITE
PS01326 DAP_EPIMERASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q491Z7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRFTKMNGLG NDFVIIDAVT QNIHLTSENI RYLSDRFYGV GFDQLLIVEP
60 70 80 90 100
PYDPAIDFHC RIYNSDGTEV NQCGNGMRCV AQFVCLKKLT NKRNIHISTR
110 120 130 140 150
ANHIILSIMN DNRVSVNMGA PIFDPKLIPF YISQYQKTYI LFLPTQIILC
160 170 180 190 200
GVVSMGNPHC IILVEKIENI QVTSLGSALE DHHCFPERVN VSFMQIINCN
210 220 230 240 250
NIRLRVYERG VGETQACGTA ACAAVAVGIQ QGLLYESVNV NLPGGTISVN
260 270
WKGASNALYM TGSTSYVYDG YINL
Length:274
Mass (Da):30,516
Last modified:September 13, 2005 - v1
Checksum:i40584B6CCB19BD22
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000016 Genomic DNA Translation: AAZ41205.1
RefSeqiWP_011283116.1, NC_007292.1

Genome annotation databases

EnsemblBacteriaiAAZ41205; AAZ41205; BPEN_600
KEGGibpn:BPEN_600

Similar proteinsi

Entry informationi

Entry nameiDAPF_BLOPB
AccessioniPrimary (citable) accession number: Q491Z7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: September 13, 2005
Last modified: May 23, 2018
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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