Q491Z0 (ILVD_BLOPB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	BPEN_611

Organism

Blochmannia pennsylvanicus (strain BPEN) [Complete proteome] [HAMAP]

Taxonomic identifier

291272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › ant endosymbionts › Candidatus Blochmannia

Protein attributes

Sequence length	616 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 616	616	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_0000225374
Sites
	Metal binding	122	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	195	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q491Z0 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 355A7ABDFC876AA6	FASTA	616	66,673
	10 20 30 40 50 60 MPKYRSITTT HGRNMTGARA LWRATGMTTE DFDKMIIAVV NSFTQFVPGH IHLRNVGALV 70 80 90 100 110 120 SEQINITGGV AKEFNTIAID DGIAMGHSGM LYSLPSRDLI ADSVEYMINA HCVDAMVCIS 130 140 150 160 170 180 NCDKITPGML MAALRLNIPA IFVSGGPMES GAITLSNQNV KLNLIDAITC SVDPNISDVD 190 200 210 220 230 240 QQRIESAACP TCGSCSGMFT ANSMNCLTEA LGLAQPGNGS LLATHSDRKK LFLNAGKYIV 250 260 270 280 290 300 HLAKSYYEEN NYSVLPRNIA NKSSFENAMM LDIAMGGSTN TVLHLLAAAQ EGEIDFTMAD 310 320 330 340 350 360 IDKLSRKVPH LCKVAPNTQR YHMEDFHRAG GVMGVLGELH RCGLLHEDTR NILNKSLLET 370 380 390 400 410 420 LLNYDIFSCD NFESKNMYAA APAGIRSTQA FIQKNRWTSL DTDRRSGCIR SREHAYSQDG 430 440 450 460 470 480 GLAVLYGNLA VDGCLVKTAG VHINLQKFSG PAKVYESQEE SVQAILTGNI HSGDVIVIRY 490 500 510 520 530 540 EGPKGGPGMQ EMLYPTSFLK SMGLDLCCAL ITDGRFSGGT SGLSIGHISP EAANKGLIGL 550 560 570 580 590 600 VHDGDIINID ISMRSITLEV SEHILKIRHA AEIARGNKAW TPTNRNRNIS ISLKAYAHLV 610 TSADKGAVRD KSKLLG

References

[1]

"Genome sequence of Blochmannia pennsylvanicus indicates parallel evolutionary trends among bacterial mutualists of insects." Degnan P.H., Lazarus A.B., Wernegreen J.J. Genome Res. 15:1023-1033(2005) [PubMed: 16077009] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BPEN.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000016 Genomic DNA. Translation: AAZ41212.1.
RefSeq	YP_278089.1. NC_007292.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q491Z0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3563084.
GenomeReviews	Gene locus BPEN_611 in contig CP000016_GR.
KEGG	bpn:BPEN_611.
PATRIC	31966159. VBICanBlo110049_0596.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0129.
HOGENOM	HBG671001.
OMA	MALVIRY.
PhylomeDB	Q491Z0.
ProtClustDB	PRK12448.
Enzyme and pathway databases
BioCyc	CBLO291272:BPEN_611-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_BLOPB

Accession

Primary (citable) accession number: Q491Z0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	September 13, 2005
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program