Reviewed, UniProtKB/Swiss-Prot Q491V4 (METE_BLOPB)

Names and origin

Protein names

Recommended name:
    5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
    EC=2.1.1.14
Alternative name(s):
    Methionine synthase, vitamin-B12 independent isozyme
    Cobalamin-independent methionine synthase

Gene names

Name:	metE
Ordered Locus Names:	BPEN_652

Organism

Blochmannia pennsylvanicus (strain BPEN) [Complete proteome] [HAMAP]

Taxonomic identifier

291272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › ant endosymbionts › Candidatus Blochmannia

Protein attributes

Sequence length	764 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity.
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 764	764	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000017226
Sites
	Metal binding	647	1	Zinc By similarity
	Metal binding	649	1	Zinc By similarity
	Metal binding	733	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q491V4-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 604C5B15EFD63ADE	FASTA	764	89,003
	10 20 30 40 50 60 MTIVGHILGF PRIGLHRELK YALEYYWDGK IKEDKLLEIG RTLRIRHWKQ QKDSGIDWIS 70 80 90 100 110 120 VGDFAWYDHV LTTSLMLNNI PDRHRIGQEN INLNTLFRIS RGYSLDKKLI PPSEMKKWFN 130 140 150 160 170 180 TNYHYIVPEF VPNQEFKLNW TQLFDEIDEA LSLKYTVKPI LLGPISYLWL GKIKGKKFDR 190 200 210 220 230 240 LSLLPNLLSV YQEILDILLS KNIDWIQIDE PILVLELPLQ WLNAYLSAYE QLHGKVKLLL 250 260 270 280 290 300 TTYFDSIYHQ LDIITRLKID GLHVDLVSNP DNIPLLHARL PKHWTLSAGV VNGRNVWKTN 310 320 330 340 350 360 LHDWFKKLYP LVGERSLWIG SSCSLLHSPI DLNTEIKLDK HIKEWFAFAL QKCYEIKMLC 370 380 390 400 410 420 AALNAENTSD ATYEDTLSEY YISNNLRSYS STIHNIQVKK RLEHIDEISY SRSSPYTTRV 430 440 450 460 470 480 TLQHKKLNLP LFPTTTIGSF PQTEEIRKLR SRFKNNQINK EDYHLNIKNY IKKIIMEQEK 490 500 510 520 530 540 LDLDILVHGE PERNDMVEYF GENLNGFIFT QNGWVQSYGS RCVKPPVIIG DISRSKPITI 550 560 570 580 590 600 EWISYAQSLT NKPIKGILTG PVTIMSWSFP REDMQRKTIA LQLALSIRDE VIDLEKSGIG 610 620 630 640 650 660 IIQIDEPALR EGLPLKRSDQ KQYLEWAINV FRITVSSVKD DTQIHTHMCY SEFSDIIYAI 670 680 690 700 710 720 LALDADVISI EAARSDEKIL KSIRHVIKNL NEIGPGIYDI HSPNKPTQND LIRRINQLLK 730 740 750 760 YIPEQRLWIN PDCGLKTRSW PEIKHSLNNM VSAAKLLRKQ FYSS

References

[1]	"Genome sequence of Blochmannia pennsylvanicus indicates parallel evolutionary trends among bacterial mutualists of insects." Degnan P.H., Lazarus A.B., Wernegreen J.J. Genome Res. 15:1023-1033(2005) [PubMed: 16077009] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000016 Genomic DNA. Translation: AAZ41250.1.
RefSeq	YP_278127.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q491V4.
Genome annotation databases
GeneID	3562729.
GenomeReviews	Gene locus BPEN_652 in contig CP000016_GR.
KEGG	bpn:BPEN_652.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q491V4.
OMA	CSLLHTP.
Enzyme and pathway databases
BioCyc	CBLO291272:BPEN_652-MON.
Family and domain databases
HAMAP	MF_00172. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR002629. Methionine_synth. IPR006276. MeTrfase_B12_ind. [Graphical view]
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
ProDom	PD004692. Methionine_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01371. met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_BLOPB

Accession

Primary (citable) accession number: Q491V4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 13, 2005
Last modified:	November 3, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)