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Q49184 (DHP1_MYCFO) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dihydropteroate synthase type-1

EC=2.5.1.15
Alternative name(s):
Dihydropteroate pyrophosphorylase type I
Dihydropteroate synthase type I
Short name=DHPS
Gene names
Name:sulI
Synonyms:suf3
OrganismMycobacterium fortuitum
Taxonomic identifier1766 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium

Protein attributes

Sequence length283 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DHPS catalyzes the formation of the immediate precursor of folic acid. The type II enzyme is stable whereas type I DHPS loses its activity rapidly By similarity. Implicated in resistance to sulfonamide.

Catalytic activity

(2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate.

Cofactor

Binds 1 magnesium ion per subunit. Magnesium is required for activity, even if it interacts primarily with the substrate By similarity.

Pathway

Cofactor biosynthesis; tetrahydrofolate biosynthesis; 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate: step 1/2.

Subunit structure

Homodimer or homotrimer By similarity.

Miscellaneous

The sulI gene is located on various large self-transmissible resistance plasmids and on transposons related to Tn21.

Sequence similarities

Belongs to the DHPS family.

Contains 1 pterin-binding domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 283283Dihydropteroate synthase type-1
PRO_0000321868

Regions

Domain6 – 262257Pterin-binding
Region53 – 542Substrate binding By similarity

Sites

Metal binding131Magnesium By similarity
Binding site211Substrate By similarity
Binding site861Substrate By similarity
Binding site1051Substrate By similarity
Binding site1771Substrate By similarity
Binding site2161Substrate By similarity
Binding site2501Substrate By similarity
Binding site2521Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q49184 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: F04DB17FB1A4951E

FASTA28330,639
        10         20         30         40         50         60 
MLRSRVTVFG ILNLTEDSFF DESRRLDPAG AVTAAIEMLR VGSDVVDVGP AASHPDARPV 

        70         80         90        100        110        120 
SPADEIRRIA PLLDALSDQM HRVSIDSFQP ETQRYALKRG VGYLNDIQGF PDPALYPDIA 

       130        140        150        160        170        180 
EADCRLVVMH SAQRDGIATR TGHLRPEDAL DEIVRFFEAR VSALRRSGVA ADRLILDPGM 

       190        200        210        220        230        240 
GFFLSPAPET SLHVLSNLQK LKSALGLPLL VSVSRKSFLG ATVGLPVKDL GPASLAAELH 

       250        260        270        280 
AIGNGADYVR THAPGDLRSA ITFSETLAKF RSRDARDRGL DHA 

« Hide

References

[1]"Transposition of an antibiotic resistance element in mycobacteria."
Martin C., Timm J., Rauzier J., Gomez-Lus R., Davies J., Gicquel B.
Nature 345:739-743(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INVOLVEMENT IN RESISTANCE TO SULFONAMIDE.
Strain: FC1.
Transposon: Tn610.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X53635 Genomic DNA. Translation: CAA37684.1.
PIRS10928.

3D structure databases

ProteinModelPortalQ49184.
ModBaseSearch...
MobiDBSearch...

Chemistry

DrugBankDB00891. Sulfapyridine.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00077; UER00156.

Family and domain databases

Gene3D3.20.20.20. 1 hit.
InterProIPR006390. DHP_synth.
IPR011005. Dihydropteroate_synth-like.
IPR000489. Pterin-binding.
[Graphical view]
PfamPF00809. Pterin_bind. 1 hit.
[Graphical view]
SUPFAMSSF51717. SSF51717. 1 hit.
TIGRFAMsTIGR01496. DHPS. 1 hit.
PROSITEPS00792. DHPS_1. 1 hit.
PS00793. DHPS_2. 1 hit.
PS50972. PTERIN_BINDING. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDHP1_MYCFO
AccessionPrimary (citable) accession number: Q49184
Entry history
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: November 1, 1996
Last modified: December 11, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways