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Protein

Dihydropteroate synthase type-1

Gene

sulI

Organism
Mycobacterium fortuitum
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

DHPS catalyzes the formation of the immediate precursor of folic acid. The type II enzyme is stable whereas type I DHPS loses its activity rapidly (By similarity). Implicated in resistance to sulfonamide.By similarity

Catalytic activityi

6-hydroxymethyl-7,8-dihydropterin diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit. Magnesium is required for activity, even if it interacts primarily with the substrate.By similarity

Pathway:itetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Dihydropteroate synthase type-1 (sulI)
  2. no protein annotated in this organism
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi13 – 131MagnesiumBy similarity
Binding sitei21 – 211SubstrateBy similarity
Binding sitei86 – 861SubstrateBy similarity
Binding sitei105 – 1051SubstrateBy similarity
Binding sitei177 – 1771SubstrateBy similarity
Binding sitei216 – 2161SubstrateBy similarity
Binding sitei250 – 2501SubstrateBy similarity
Binding sitei252 – 2521SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Antibiotic resistance, Folate biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00077; UER00156.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydropteroate synthase type-1 (EC:2.5.1.15)
Alternative name(s):
Dihydropteroate pyrophosphorylase type I
Dihydropteroate synthase type I
Short name:
DHPS
Gene namesi
Name:sulI
Synonyms:suf3
OrganismiMycobacterium fortuitum
Taxonomic identifieri1766 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium

Pathology & Biotechi

Chemistry

DrugBankiDB00891. Sulfapyridine.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 283283Dihydropteroate synthase type-1PRO_0000321868Add
BLAST

Interactioni

Subunit structurei

Homodimer or homotrimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ49184.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini6 – 262257Pterin-bindingPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni53 – 542Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the DHPS family.Curated
Contains 1 pterin-binding domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.20.20.20. 1 hit.
InterProiIPR006390. DHP_synth.
IPR011005. Dihydropteroate_synth-like.
IPR000489. Pterin-binding.
[Graphical view]
PfamiPF00809. Pterin_bind. 1 hit.
[Graphical view]
SUPFAMiSSF51717. SSF51717. 1 hit.
TIGRFAMsiTIGR01496. DHPS. 1 hit.
PROSITEiPS00792. DHPS_1. 1 hit.
PS00793. DHPS_2. 1 hit.
PS50972. PTERIN_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q49184-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRSRVTVFG ILNLTEDSFF DESRRLDPAG AVTAAIEMLR VGSDVVDVGP
60 70 80 90 100
AASHPDARPV SPADEIRRIA PLLDALSDQM HRVSIDSFQP ETQRYALKRG
110 120 130 140 150
VGYLNDIQGF PDPALYPDIA EADCRLVVMH SAQRDGIATR TGHLRPEDAL
160 170 180 190 200
DEIVRFFEAR VSALRRSGVA ADRLILDPGM GFFLSPAPET SLHVLSNLQK
210 220 230 240 250
LKSALGLPLL VSVSRKSFLG ATVGLPVKDL GPASLAAELH AIGNGADYVR
260 270 280
THAPGDLRSA ITFSETLAKF RSRDARDRGL DHA
Length:283
Mass (Da):30,639
Last modified:November 1, 1996 - v1
Checksum:iF04DB17FB1A4951E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53635 Genomic DNA. Translation: CAA37684.1.
PIRiS10928.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53635 Genomic DNA. Translation: CAA37684.1.
PIRiS10928.

3D structure databases

ProteinModelPortaliQ49184.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

DrugBankiDB00891. Sulfapyridine.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00077; UER00156.

Family and domain databases

Gene3Di3.20.20.20. 1 hit.
InterProiIPR006390. DHP_synth.
IPR011005. Dihydropteroate_synth-like.
IPR000489. Pterin-binding.
[Graphical view]
PfamiPF00809. Pterin_bind. 1 hit.
[Graphical view]
SUPFAMiSSF51717. SSF51717. 1 hit.
TIGRFAMsiTIGR01496. DHPS. 1 hit.
PROSITEiPS00792. DHPS_1. 1 hit.
PS00793. DHPS_2. 1 hit.
PS50972. PTERIN_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Transposition of an antibiotic resistance element in mycobacteria."
    Martin C., Timm J., Rauzier J., Gomez-Lus R., Davies J., Gicquel B.
    Nature 345:739-743(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INVOLVEMENT IN RESISTANCE TO SULFONAMIDE.
    Strain: FC1.
    Transposon: Tn610.

Entry informationi

Entry nameiDHP1_MYCFO
AccessioniPrimary (citable) accession number: Q49184
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: November 1, 1996
Last modified: June 24, 2015
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The sulI gene is located on various large self-transmissible resistance plasmids and on transposons related to Tn21.

Keywords - Technical termi

Transposable element

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.