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Q49174 (MCRX_METFV) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Methyl-coenzyme M reductase II subunit alpha

Short name=MCR II alpha
EC=2.8.4.1
Alternative name(s):
Coenzyme-B sulfoethylthiotransferase alpha
Gene names
Name:mrtA
Synonyms:mcrIIA
Ordered Locus Names:Mfer_0734
OrganismMethanothermus fervidus (strain ATCC 43054 / DSM 2088 / JCM 10308 / V24 S) [Complete proteome] [HAMAP]
Taxonomic identifier523846 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanothermaceaeMethanothermus

Protein attributes

Sequence length554 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Reduction of methyl-coenzyme M (2-(methylthio) ethanesulfonic acid) with 7-mercaptoheptanoylthreonine phosphate to methane and a heterodisulfide By similarity.

Catalytic activity

Methyl-CoM + CoB = CoM-S-S-CoB + methane.

Cofactor

Binds 2 coenzyme F430 noncovalently per hexamer. Coenzyme F430 is a yellow nickel porphinoid By similarity.

Pathway

One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.

Subunit structure

Hexamer of two alpha, two beta, and two gamma chains By similarity.

Ontologies

Keywords
   Biological processMethanogenesis
   LigandMetal-binding
Nickel
   Molecular functionTransferase
   PTMMethylation
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processmethanogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functioncoenzyme-B sulfoethylthiotransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 554554Methyl-coenzyme M reductase II subunit alpha
PRO_0000147451

Sites

Metal binding1511Nickel By similarity

Amino acid modifications

Modified residue2611Pros-methylhistidine By similarity
Modified residue27415-methylarginine By similarity

Experimental info

Sequence conflict2731A → G in CAA50044. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q49174 [UniParc].

Last modified March 8, 2011. Version 2.
Checksum: E894701807E22919

FASTA55461,326
        10         20         30         40         50         60 
MNKKNKKLFL EALEKKFKGE SPEEKKTTFY CFGGWKQSER KREFVEYAKK LAKKRGIPFY 

        70         80         90        100        110        120 
NPDIGVPLGQ RKLMAYRISG TDAYVEGDDL HFVNNAAIQQ MVDDIKRTVI VGMDTAHAVL 

       130        140        150        160        170        180 
EKRLGVEVTP ETINEYMETI NHALPGGAVV QEHMVEVHPG LVDDCYAKIF TGNDELADEL 

       190        200        210        220        230        240 
DKRVLIDINK EFPEEQAEML KKYIGNRTYQ VNRVPTIVVR CCDGGTVSRW SAMQIGMSFI 

       250        260        270        280        290        300 
SAYKLCAGEA AIADFSFAAK HADVIEMGTI LPARRARGPN EPGGIPFGVF ADIIQTSRVS 

       310        320        330        340        350        360 
DDPARISLEV IGAAATLYDQ VWLGSYMSGG VGFTQYASAT YTDDILDDFV YYGAEYVEDK 

       370        380        390        400        410        420 
YGFCGVKPSM EVVKDIATEV TLYGLEQYEE YPTLLEDHFG GSQRAAVVAA AAGCSTAFAT 

       430        440        450        460        470        480 
GNSNAGINAW YLSQILHKEG HSRLGFYGYD LQDQCGASNS LSIRSDEGLV HELRGPNYPN 

       490        500        510        520        530        540 
YAMNVGHQPE YAGIAQAPHA ARGDAFVVNP LIKVAFADND LSFDFRWPRK EIARGALREF 

       550 
MPDGERTLII PASK 

« Hide

References

« Hide 'large scale' references
[1]"Characterization and phylogeny of mcrII, a gene cluster encoding an isoenzyme of methyl coenzyme M reductase from hyperthermophilic Methanothermus fervidus."
Lehmacher A., Klenk H.-P.
Mol. Gen. Genet. 243:198-206(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 43054 / DSM 2088 / JCM 10308 / V24 S.
[2]"Complete genome sequence of Methanothermus fervidus type strain (V24S)."
Anderson I., Djao O.D., Misra M., Chertkov O., Nolan M., Lucas S., Lapidus A., Del Rio T.G., Tice H., Cheng J.F., Tapia R., Han C., Goodwin L., Pitluck S., Liolios K., Ivanova N., Mavromatis K., Mikhailova N. expand/collapse author list , Pati A., Brambilla E., Chen A., Palaniappan K., Land M., Hauser L., Chang Y.J., Jeffries C.D., Sikorski J., Spring S., Rohde M., Eichinger K., Huber H., Wirth R., Goker M., Detter J.C., Woyke T., Bristow J., Eisen J.A., Markowitz V., Hugenholtz P., Klenk H.P., Kyrpides N.C.
Stand. Genomic Sci. 3:315-324(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43054 / DSM 2088 / JCM 10308 / V24 S.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X70765 Genomic DNA. Translation: CAA50044.1.
CP002278 Genomic DNA. Translation: ADP77533.1.
PIRS43897.
RefSeqYP_004004295.1. NC_014658.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADP77533; ADP77533; Mfer_0734.
GeneID9962464.
KEGGmfv:Mfer_0734.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000225809.
KOK00399.
OMAGHQPEYA.

Enzyme and pathway databases

BioCycMFER523846:GC24-763-MONOMER.
UniPathwayUPA00646; UER00699.

Family and domain databases

Gene3D1.20.840.10. 1 hit.
3.30.70.470. 1 hit.
3.90.390.10. 1 hit.
InterProIPR022681. MCR_a/b_chain_a-bundle.
IPR016212. Me_CoM_Rdtase_asu.
IPR008924. Me_CoM_Rdtase_asu/bsu_C.
IPR009047. Me_CoM_Rdtase_asu_C.
IPR003183. Me_CoM_Rdtase_asu_N.
IPR015811. Me_CoM_Rdtase_asu_N_sub1.
IPR015823. Me_CoM_Rdtase_asu_N_sub2.
IPR009024. Me_CoM_Rdtase_Fd-like_fold.
[Graphical view]
PfamPF02249. MCR_alpha. 1 hit.
PF02745. MCR_alpha_N. 1 hit.
[Graphical view]
PIRSFPIRSF000262. MCR_alpha. 1 hit.
SUPFAMSSF48081. SSF48081. 1 hit.
SSF55088. SSF55088. 1 hit.
TIGRFAMsTIGR03256. met_CoM_red_alp. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMCRX_METFV
AccessionPrimary (citable) accession number: Q49174
Secondary accession number(s): E3GZ01
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: March 8, 2011
Last modified: October 16, 2013
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways