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Q49174

- MCRX_METFV

UniProt

Q49174 - MCRX_METFV

Protein

Methyl-coenzyme M reductase II subunit alpha

Gene

mrtA

Organism
Methanothermus fervidus (strain ATCC 43054 / DSM 2088 / JCM 10308 / V24 S)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 2 (08 Mar 2011)
      Previous versions | rss
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    Functioni

    Reduction of methyl-coenzyme M (2-(methylthio) ethanesulfonic acid) with 7-mercaptoheptanoylthreonine phosphate to methane and a heterodisulfide.By similarity

    Catalytic activityi

    Methyl-CoM + CoB = CoM-S-S-CoB + methane.

    Cofactori

    Binds 1 coenzyme F430 noncovalently per subunit. Coenzyme F430 is a yellow nickel porphinoid By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi151 – 1511NickelBy similarity

    GO - Molecular functioni

    1. coenzyme-B sulfoethylthiotransferase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. methanogenesis Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Methanogenesis

    Keywords - Ligandi

    Metal-binding, Nickel

    Enzyme and pathway databases

    BioCyciMFER523846:GC24-763-MONOMER.
    UniPathwayiUPA00646; UER00699.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methyl-coenzyme M reductase II subunit alpha (EC:2.8.4.1)
    Short name:
    MCR II alpha
    Alternative name(s):
    Coenzyme-B sulfoethylthiotransferase alpha
    Gene namesi
    Name:mrtA
    Synonyms:mcrIIA
    Ordered Locus Names:Mfer_0734
    OrganismiMethanothermus fervidus (strain ATCC 43054 / DSM 2088 / JCM 10308 / V24 S)
    Taxonomic identifieri523846 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanothermaceaeMethanothermus
    ProteomesiUP000002315: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 554554Methyl-coenzyme M reductase II subunit alphaPRO_0000147451Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei261 – 2611Pros-methylhistidineBy similarity
    Modified residuei275 – 27515-methylarginineBy similarity

    Keywords - PTMi

    Methylation

    Interactioni

    Subunit structurei

    Hexamer of two alpha, two beta, and two gamma chains.By similarity

    Family & Domainsi

    Phylogenomic databases

    HOGENOMiHOG000225809.
    KOiK00399.
    OMAiGHQPEYA.

    Family and domain databases

    Gene3Di1.20.840.10. 1 hit.
    3.30.70.470. 1 hit.
    3.90.390.10. 1 hit.
    InterProiIPR022681. MCR_a/b_chain_a-bundle.
    IPR016212. Me_CoM_Rdtase_asu.
    IPR008924. Me_CoM_Rdtase_asu/bsu_C.
    IPR009047. Me_CoM_Rdtase_asu_C.
    IPR003183. Me_CoM_Rdtase_asu_N.
    IPR015811. Me_CoM_Rdtase_asu_N_sub1.
    IPR015823. Me_CoM_Rdtase_asu_N_sub2.
    IPR009024. Me_CoM_Rdtase_Fd-like_fold.
    [Graphical view]
    PfamiPF02249. MCR_alpha. 1 hit.
    PF02745. MCR_alpha_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000262. MCR_alpha. 1 hit.
    SUPFAMiSSF48081. SSF48081. 1 hit.
    SSF55088. SSF55088. 1 hit.
    TIGRFAMsiTIGR03256. met_CoM_red_alp. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q49174-1 [UniParc]FASTAAdd to Basket

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    MNKKNKKLFL EALEKKFKGE SPEEKKTTFY CFGGWKQSER KREFVEYAKK    50
    LAKKRGIPFY NPDIGVPLGQ RKLMAYRISG TDAYVEGDDL HFVNNAAIQQ 100
    MVDDIKRTVI VGMDTAHAVL EKRLGVEVTP ETINEYMETI NHALPGGAVV 150
    QEHMVEVHPG LVDDCYAKIF TGNDELADEL DKRVLIDINK EFPEEQAEML 200
    KKYIGNRTYQ VNRVPTIVVR CCDGGTVSRW SAMQIGMSFI SAYKLCAGEA 250
    AIADFSFAAK HADVIEMGTI LPARRARGPN EPGGIPFGVF ADIIQTSRVS 300
    DDPARISLEV IGAAATLYDQ VWLGSYMSGG VGFTQYASAT YTDDILDDFV 350
    YYGAEYVEDK YGFCGVKPSM EVVKDIATEV TLYGLEQYEE YPTLLEDHFG 400
    GSQRAAVVAA AAGCSTAFAT GNSNAGINAW YLSQILHKEG HSRLGFYGYD 450
    LQDQCGASNS LSIRSDEGLV HELRGPNYPN YAMNVGHQPE YAGIAQAPHA 500
    ARGDAFVVNP LIKVAFADND LSFDFRWPRK EIARGALREF MPDGERTLII 550
    PASK 554
    Length:554
    Mass (Da):61,326
    Last modified:March 8, 2011 - v2
    Checksum:iE894701807E22919
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti273 – 2731A → G in CAA50044. (PubMed:8177216)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X70765 Genomic DNA. Translation: CAA50044.1.
    CP002278 Genomic DNA. Translation: ADP77533.1.
    PIRiS43897.
    RefSeqiWP_013413811.1. NC_014658.1.
    YP_004004295.1. NC_014658.1.

    Genome annotation databases

    EnsemblBacteriaiADP77533; ADP77533; Mfer_0734.
    GeneIDi9962464.
    KEGGimfv:Mfer_0734.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X70765 Genomic DNA. Translation: CAA50044.1 .
    CP002278 Genomic DNA. Translation: ADP77533.1 .
    PIRi S43897.
    RefSeqi WP_013413811.1. NC_014658.1.
    YP_004004295.1. NC_014658.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ADP77533 ; ADP77533 ; Mfer_0734 .
    GeneIDi 9962464.
    KEGGi mfv:Mfer_0734.

    Phylogenomic databases

    HOGENOMi HOG000225809.
    KOi K00399.
    OMAi GHQPEYA.

    Enzyme and pathway databases

    UniPathwayi UPA00646 ; UER00699 .
    BioCyci MFER523846:GC24-763-MONOMER.

    Family and domain databases

    Gene3Di 1.20.840.10. 1 hit.
    3.30.70.470. 1 hit.
    3.90.390.10. 1 hit.
    InterProi IPR022681. MCR_a/b_chain_a-bundle.
    IPR016212. Me_CoM_Rdtase_asu.
    IPR008924. Me_CoM_Rdtase_asu/bsu_C.
    IPR009047. Me_CoM_Rdtase_asu_C.
    IPR003183. Me_CoM_Rdtase_asu_N.
    IPR015811. Me_CoM_Rdtase_asu_N_sub1.
    IPR015823. Me_CoM_Rdtase_asu_N_sub2.
    IPR009024. Me_CoM_Rdtase_Fd-like_fold.
    [Graphical view ]
    Pfami PF02249. MCR_alpha. 1 hit.
    PF02745. MCR_alpha_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000262. MCR_alpha. 1 hit.
    SUPFAMi SSF48081. SSF48081. 1 hit.
    SSF55088. SSF55088. 1 hit.
    TIGRFAMsi TIGR03256. met_CoM_red_alp. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Characterization and phylogeny of mcrII, a gene cluster encoding an isoenzyme of methyl coenzyme M reductase from hyperthermophilic Methanothermus fervidus."
      Lehmacher A., Klenk H.-P.
      Mol. Gen. Genet. 243:198-206(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 43054 / DSM 2088 / JCM 10308 / V24 S.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43054 / DSM 2088 / JCM 10308 / V24 S.

    Entry informationi

    Entry nameiMCRX_METFV
    AccessioniPrimary (citable) accession number: Q49174
    Secondary accession number(s): E3GZ01
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 25, 2003
    Last sequence update: March 8, 2011
    Last modified: October 1, 2014
    This is version 85 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways

    External Data

    Dasty 3