Q49173 (MCRZ_METFV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methyl-coenzyme M reductase II subunit gamma
Short name=MCR II gamma
EC=2.8.4.1

Alternative name(s):
Coenzyme-B sulfoethylthiotransferase gamma

Gene names

Name:	mrtG
Synonyms:	mcrIIG
Ordered Locus Names:	Mfer_0733

Organism

Methanothermus fervidus (strain ATCC 43054 / DSM 2088 / JCM 10308 / V24 S) [Complete proteome] [HAMAP]

Taxonomic identifier

523846 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanothermaceae › Methanothermus

Protein attributes

Sequence length	267 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Reduction of methyl-coenzyme M (2-(methylthio) ethanesulfonic acid) with 7-mercaptoheptanoylthreonine phosphate to methane and an heterodisulfide.
Catalytic activity	Methyl-CoM + CoB = CoM-S-S-CoB + methane.
Cofactor	Binds 2 coenzyme F430 noncovalently per hexamer. Coenzyme F430 is a yellow nickel porphinoid By similarity.
Pathway	One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.
Subunit structure	Hexamer of two alpha, two beta, and two gamma chains By similarity.

Ontologies

Keywords
Biological process	Methanogenesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	coenzyme-B sulfoethylthiotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 267	267	Methyl-coenzyme M reductase II subunit gamma		PRO_0000147475

Sequences

	Sequence		Length	Mass (Da)
	Q49173 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 150D5FFAE6BC6F9D	FASTA	267	30,847
	10 20 30 40 50 60 MAYEPQFNPG ETKIAENRRK HMNPNYELKK LREIADEDIV RVLGHRSPGE SFKTVHPPLE 70 80 90 100 110 120 EMDFEEDPMK DIVEPIEGAK QGTRIRYIQF ADSMYNAPAQ PYDRARTYMW RFRGVDTGTL 130 140 150 160 170 180 SGRQVIEMRE LDLEKVSKIL LETEIFDPAR CGIRGATVHG HSLRFDENGL MFDALQRYIY 190 200 210 220 230 240 DEDSGHVVYV KDQVGRPLDQ PVDMGEPLPE DELKEITTIY RKDNIGMRED EELLEVVNKI 250 260 HEARTIGGFG MEVFKKDLNK RLGGANE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization and phylogeny of mcrII, a gene cluster encoding an isoenzyme of methyl coenzyme M reductase from hyperthermophilic Methanothermus fervidus." Lehmacher A., Klenk H.-P. Mol. Gen. Genet. 243:198-206(1994) [PubMed: 8177216] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 43054 / DSM 2088 / JCM 10308 / V24 S.
[2]	"Complete genome sequence of Methanothermus fervidus type strain (V24S)." Anderson I., Djao O.D., Misra M., Chertkov O., Nolan M., Lucas S., Lapidus A., Del Rio T.G., Tice H., Cheng J.F., Tapia R., Han C., Goodwin L., Pitluck S., Liolios K., Ivanova N., Mavromatis K., Mikhailova N. , Pati A., Brambilla E., Chen A., Palaniappan K., Land M., Hauser L., Chang Y.J., Jeffries C.D., Sikorski J., Spring S., Rohde M., Eichinger K., Huber H., Wirth R., Goker M., Detter J.C., Woyke T., Bristow J., Eisen J.A., Markowitz V., Hugenholtz P., Klenk H.P., Kyrpides N.C. Stand. Genomic Sci. 3:315-324(2010) [PubMed: 21304736] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43054 / DSM 2088 / JCM 10308 / V24 S.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X70765 Genomic DNA. Translation: CAA50043.1. CP002278 Genomic DNA. Translation: ADP77532.1.
PIR	S43900.
RefSeq	YP_004004294.1. NC_014658.1.
3D structure databases
ProteinModelPortal	Q49173.
SMR	Q49173. Positions 6-251.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	9962463.
GenomeReviews	Gene locus Mfer_0733 in contig CP002278_GR.
KEGG	mfv:Mfer_0733.
Organism-specific databases
CMR	Search...
Family and domain databases
InterPro	IPR009024. Me_CoM_Rdtase_Fd-like_fold. IPR003178. Me_CoM_Rdtase_gsu. [Graphical view]
Gene3D	G3DSA:3.90.320.20. MCR_gamma. 1 hit.
KO	K00402.
Pfam	PF02240. MCR_gamma. 1 hit. [Graphical view]
PIRSF	PIRSF000264. Meth_CoM_rd_gama. 1 hit.
ProDom	PD005845. Me_CoM_Rdtase_gsu. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF55088. MCR_fer_like. 1 hit.
TIGRFAMs	TIGR03259. Met_CoM_red_gam. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MCRZ_METFV

Accession

Primary (citable) accession number: Q49173
Secondary accession number(s): E3GZ00

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2003
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program