Reviewed, UniProtKB/Swiss-Prot Q49173 (MCRZ_METFE)

Names and origin

Protein names

Recommended name:
    Methyl-coenzyme M reductase II subunit gamma
      Short name=MCR II gamma
    EC=2.8.4.1
Alternative name(s):
    Coenzyme-B sulfoethylthiotransferase gamma

Gene names

Name:	mrtG
Synonyms:	mcrIIG

Organism

Methanothermus fervidus

Taxonomic identifier

2180 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanothermaceae › Methanothermus

Protein attributes

Sequence length	267 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduction of methyl-coenzyme M (2-(methylthio) ethanesulfonic acid) with 7-mercaptoheptanoylthreonine phosphate to methane and an heterodisulfide.
Catalytic activity	2-(methylthio)ethanesulfonate (methyl-CoM) + N-(7-mercaptoheptanoyl)threonine 3-O-phosphate (coenzyme B) = CoM-S-S-CoB + methane.
Cofactor	Binds 2 coenzyme F430 noncovalently per hexamer. Coenzyme F430 is a yellow nickel porphinoid By similarity.
Pathway	One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.
Subunit structure	Hexamer of two alpha, two beta, and two gamma chains By similarity.

Ontologies

Keywords
Biological process	Methanogenesis
Molecular function	Transferase
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	coenzyme-B sulfoethylthiotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 267	267	Methyl-coenzyme M reductase II subunit gamma		PRO_0000147475

Sequences

	Sequence		Length	Mass (Da)
	Q49173-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 150D5FFAE6BC6F9D	FASTA	267	30,847
	10 20 30 40 50 60 MAYEPQFNPG ETKIAENRRK HMNPNYELKK LREIADEDIV RVLGHRSPGE SFKTVHPPLE 70 80 90 100 110 120 EMDFEEDPMK DIVEPIEGAK QGTRIRYIQF ADSMYNAPAQ PYDRARTYMW RFRGVDTGTL 130 140 150 160 170 180 SGRQVIEMRE LDLEKVSKIL LETEIFDPAR CGIRGATVHG HSLRFDENGL MFDALQRYIY 190 200 210 220 230 240 DEDSGHVVYV KDQVGRPLDQ PVDMGEPLPE DELKEITTIY RKDNIGMRED EELLEVVNKI 250 260 HEARTIGGFG MEVFKKDLNK RLGGANE

References

[1]

"Characterization and phylogeny of mcrII, a gene cluster encoding an isoenzyme of methyl coenzyme M reductase from hyperthermophilic Methanothermus fervidus." Lehmacher A., Klenk H.-P. Mol. Gen. Genet. 243:198-206(1994) [PubMed: 8177216] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 2088 / V24S.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X70765 Genomic DNA. Translation: CAA50043.1.
PIR	S43900.
3D structure databases
HSSP	HSSP built from PDB template 1E6V based on UniProtKB Q49604.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.8.4.1. 7358.
Family and domain databases
InterPro	IPR003178. Me_CoM_Rdtase_gsu. [Graphical view]
Gene3D	G3DSA:3.90.320.20. MCR_gamma. 1 hit.
Pfam	PF02240. MCR_gamma. 1 hit. [Graphical view]
PIRSF	PIRSF000264. Meth_CoM_rd_gama. 1 hit.
ProDom	PD005845. MCR_gamma. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03259. met_CoM_red_gam. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MCRZ_METFE

Accession

Primary (citable) accession number: Q49173

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2003
Last sequence update:	November 1, 1996
Last modified:	September 22, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)