Q49171 (MCRY_METFV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methyl-coenzyme M reductase II subunit beta
Short name=MCR II beta
EC=2.8.4.1

Alternative name(s):
Coenzyme-B sulfoethylthiotransferase beta

Gene names

Name:	mrtB
Synonyms:	mcrIIB
Ordered Locus Names:	Mfer_0731

Organism

Methanothermus fervidus (strain ATCC 43054 / DSM 2088 / JCM 10308 / V24 S) [Complete proteome] [HAMAP]

Taxonomic identifier

523846 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanothermaceae › Methanothermus

Protein attributes

Sequence length	443 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Reduction of methyl-coenzyme M (2-(methylthio) ethanesulfonic acid) with 7-mercaptoheptanoylthreonine phosphate to methane and an heterodisulfide By similarity.
Catalytic activity	Methyl-CoM + CoB = CoM-S-S-CoB + methane.
Cofactor	Binds 2 coenzyme F430 noncovalently per hexamer. Coenzyme F430 is a yellow nickel porphinoid By similarity.
Pathway	One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.
Subunit structure	Hexamer of two alpha, two beta, and two gamma chains By similarity.

Ontologies

Keywords
Biological process	Methanogenesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	coenzyme-B sulfoethylthiotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 443	443	Methyl-coenzyme M reductase II subunit beta		PRO_0000147464

Sequences

	Sequence		Length	Mass (Da)
	Q49171 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: FEAA6DDD20A262E6	FASTA	443	47,447
	10 20 30 40 50 60 MAIYEDKIDL YDANGKLLDE NVPLEAISPL KNPTIGKIVN DVKRSVAVNL AGIENSLKKA 70 80 90 100 110 120 ALGGKANFIP GRELDLDIVE NAEIIAEKIK KMVQVDENDD TNVKMINNGQ QLLVQVPTIR 130 140 150 160 170 180 IERAADYTVS TLVAGAATIQ AIIDTFDVDM FDASTVKTAV LGRYPQTVDF TGANVAAMLS 190 200 210 220 230 240 PPVLLEGLGY GLRNILTNHI VATTKKNTLN AAALSSILEQ TAMFETGDAV GAFERLHLLG 250 260 270 280 290 300 LAYQGLNADN LVYDLVKENK KGTVGTVIAS LVERAIEDKV IKVSKEMPSG FRVYEPIDWA 310 320 330 340 350 360 LWNAYAAAGL LAATIVNIGA ARAAQGVAST VLYYNDILEY ETGLPGVDFG RAEGTAVGFS 370 380 390 400 410 420 FFSHSIYGGG GPGIFHGNHV VTRHSKGFAL PCVAAAMSLD AGTQMFSPER TSGLVGQVYS 430 440 EIDYFREPIK YVAEGAAKIK NKI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization and phylogeny of mcrII, a gene cluster encoding an isoenzyme of methyl coenzyme M reductase from hyperthermophilic Methanothermus fervidus." Lehmacher A., Klenk H.-P. Mol. Gen. Genet. 243:198-206(1994) [PubMed: 8177216] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 43054 / DSM 2088 / JCM 10308 / V24 S.
[2]	"Complete genome sequence of Methanothermus fervidus type strain (V24S)." Anderson I., Djao O.D., Misra M., Chertkov O., Nolan M., Lucas S., Lapidus A., Del Rio T.G., Tice H., Cheng J.F., Tapia R., Han C., Goodwin L., Pitluck S., Liolios K., Ivanova N., Mavromatis K., Mikhailova N. , Pati A., Brambilla E., Chen A., Palaniappan K., Land M., Hauser L., Chang Y.J., Jeffries C.D., Sikorski J., Spring S., Rohde M., Eichinger K., Huber H., Wirth R., Goker M., Detter J.C., Woyke T., Bristow J., Eisen J.A., Markowitz V., Hugenholtz P., Klenk H.P., Kyrpides N.C. Stand. Genomic Sci. 3:315-324(2010) [PubMed: 21304736] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43054 / DSM 2088 / JCM 10308 / V24 S.
[3]	Steigerwald V.J., Stroup D., Hennigan A.N., Pihl T.D., Reeve J.N. Submitted (AUG-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-125.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X70765 Genomic DNA. Translation: CAA50041.1. CP002278 Genomic DNA. Translation: ADP77530.1. M99219 Genomic DNA. Translation: AAA73384.1.
PIR	S43899.
RefSeq	YP_004004292.1. NC_014658.1.
3D structure databases
ProteinModelPortal	Q49171.
SMR	Q49171. Positions 2-443.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	9962461.
GenomeReviews	Gene locus Mfer_0731 in contig CP002278_GR.
KEGG	mfv:Mfer_0731.
Organism-specific databases
CMR	Search...
Family and domain databases
InterPro	IPR022681. MCR_a/b_chain_a-bundle. IPR008924. Me_CoM_Rdtase_asu/bsu_C. IPR015823. Me_CoM_Rdtase_asu_N_sub2. IPR003179. Me_CoM_Rdtase_bsu. IPR022679. Me_CoM_Rdtase_bsu_C. IPR022680. Me_CoM_Rdtase_bsu_N. IPR009024. Me_CoM_Rdtase_Fd-like_fold. [Graphical view]
Gene3D	G3DSA:1.20.840.10. MCR_a/b_chain_a-bundle. 2 hits. G3DSA:3.30.70.470. Me_CoM_Rdtase_asu_N_sub2. 1 hit.
KO	K00401.
Pfam	PF02241. MCR_beta. 1 hit. PF02783. MCR_beta_N. 1 hit. [Graphical view]
PIRSF	PIRSF000263. Meth_CoM_rd_beta. 1 hit.
SUPFAM	SSF48081. MCR_alpha_beta_C. 1 hit. SSF55088. MCR_fer_like. 1 hit.
TIGRFAMs	TIGR03257. Met_CoM_red_bet. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MCRY_METFV

Accession

Primary (citable) accession number: Q49171
Secondary accession number(s): E3GYZ8, Q49177

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2003
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program